GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:18441062
| Citation |
Geszvain, K and Visick, KL (2008) The hybrid sensor kinase RscS integrates positive and negative signals to modulate biofilm formation in Vibrio fischeri. J. Bacteriol. 190:4437-46 |
|---|---|
| Abstract |
Overexpression of the Vibrio fischeri sensor kinase RscS induces expression of the syp (symbiosis polysaccharide) gene cluster and promotes biofilm phenotypes such as wrinkled colony morphology, pellicle formation, and surface adherence. RscS is predicted to be a hybrid sensor kinase with a histidine kinase/ATPase (HATPase) domain, a receiver (Rec) domain, and a histidine phosphotransferase (Hpt) domain. Bioinformatic analysis also revealed the following three potential signal detection domains within RscS: two transmembrane helices forming a transmembrane region (TMR), a large periplasmic (PP) domain, and a cytoplasmic PAS domain. In this work, we genetically dissected the contributions of these domains to RscS function. Substitutions within the carboxy-terminal domain supported identification of RscS as a hybrid sensor kinase; disruption of both the HATPase and Rec domains eliminated induction of syp transcription, wrinkled colony morphology, pellicle formation, and surface adherence, while disruption of Hpt resulted in decreased activity. The PAS domain was also critical for RscS activity; substitutions in PAS resulted in a loss of activity. Generation of a cytoplasmic, N-terminal deletion derivative of RscS resulted in a partial loss of activity, suggesting a role for localization to the membrane and/or sequences within the TMR and PP domain. Finally, substitutions within the first transmembrane helix of the TMR and deletions within the PP domain both resulted in increased activity. Thus, RscS integrates both inhibitory and stimulatory signals from the environment to regulate biofilm formation by V. fischeri. |
| Links |
PubMed PMC2446786 Online version:10.1128/JB.00055-08 |
| Keywords |
Aliivibrio fischeri/growth & development; Aliivibrio fischeri/metabolism; Aliivibrio fischeri/physiology; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biofilms/growth & development; Blotting, Western; Computational Biology; Models, Genetic; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphotransferases/genetics; Phosphotransferases/metabolism; Protein Structure, Secondary; Protein Structure, Tertiary; Signal Transduction/genetics; Signal Transduction/physiology; Structure-Activity Relationship; Transcription, Genetic |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0009927: histidine phosphotransfer kinase activity |
ECO:0000315: |
F |
RscS1 if found to be an important protein in colony attachment and improve competitive fitness for colonies by encoding a histidine kinase/ATPase that regulates phosphorylation. By regulating the flow of phosphate this enzyme can possibly regulate transcription of colony attachment genes or regulate the enzymes after translation by activating or inactivating them via phosphorylation. This is shown phenotypcially with an RscS mutant if Figures 3,4,5, as well as Table 3. |
complete | ||||
|
enables |
GO:0009927: histidine phosphotransfer kinase activity |
ECO:0000315: mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
See Help:References for how to manage references in GONUTS.
