User:Re831826

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	MOUSE:PAR6B	2012-03-26 19:01:02 CDT	GO:0001831 trophectodermal cellular morphogenesis (P)	PMID:20505164	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 displays how Pard6b knockdown suppresses formation of the blastocyte cavity, although it does not affect early blastomere cleavages in embryo (Table 1).	challenge
unacceptable	?	2012-03-26 19:08:27 CDT	GO:0007155 cell adhesion (Figure 7 shows that bacteria strains induced with the sasG gene adhered to desquamated nasal epithelial cells about three times as strongly than bacteria strains lacking the gene. SasG therefore promotes cell adhesion.)	PMID:17660408	IDA: Inferred from Direct Assay		challenge
updatedbyinstructor	STAA8:SASG	2012-03-26 19:08:28 CDT	GO:0090609 single-species submerged biofilm formation (P)	PMID:17660408	ECO:0000314 direct assay evidence used in manual assertion	Figure 8 shows that SasG-expressing SH1000 cells form a biofilm measured with crystal violet staining and read at A570. SH1000 variants carrying pALC2073 expressing SasG with eight, six and five B repeats resulted in the formation of a biofilm. Strains expressing four, two or one B repeats did not form biofilms (Fig. 8c)	challenge
updatedbyinstructor	STREE:STKP	2012-03-26 19:09:01 CDT	GO:0051302 regulation of cell division (P)	PMID:22431591	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 6 and figure 7 display unbalanced cell growth and division in the absence of StkP. Wild-type cells were able to develop one cell-division ring in the center while cells with mutated strains became over-elongated and formed multiple rings, leading to division into more than two daughter cells.	challenge
unacceptable	ECOLX:E2QMH3	2012-03-26 20:06:23 CDT	GO:0006607 NLS-bearing substrate import into nucleus (A series of N- and C- terminal deletion mutants of Tus were constructed which were fused to GFP to determine the subcellular distribution of green fluorescence in the transduced cells. Figure 2A shows that the fusion of amino acids 218 to 309 of Tus to the C-terminus of GFP restored nuclear targeting, defining the NLS sequence of Tus. Further depicted in Figure 3A, alteration of any one of the basic amino acids 218-309 resulted in pronounced perturbation of nuclear targeting of the fusion proteins. )	PMID:20126275	IMP: Inferred from Mutant Phenotype		challenge
updatedbyinstructor	ECOLI:BSSS	2012-03-26 20:51:52 CDT	GO:0090609 single-species submerged biofilm formation (P)	PMID:16597943	ECO:0000315 mutant phenotype evidence used in manual assertion	Culturing of yceP mutants and wild-type strain in LB did not lead to a variation in observed biofilm formation while twofold (P = 0.052) and threefold (P = 0.001) increases in biofilm occurred in LB glu upon deletion of yceP (Figure 1A). Lastly when biofilm formation was observed in a continuous flow system in M9C glu medium, the yceP deletion caused the cells to form a more random, scattered, and globular biofilm with far less surface coverage (Figure 2B).	challenge

acceptable:0
unacceptable:2
requires_changes:1
flagged:0

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