PMID:22431591

Citation	Beilharz, K, Nováková, L, Fadda, D, Branny, P, Massidda, O and Veening, JW (2012) Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP. Proc. Natl. Acad. Sci. U.S.A. 109:E905-13
Abstract	How the human pathogen Streptococcus pneumoniae coordinates cell-wall synthesis during growth and division to achieve its characteristic oval shape is poorly understood. The conserved eukaryotic-type Ser/Thr kinase of S. pneumoniae, StkP, previously was reported to phosphorylate the cell-division protein DivIVA. Consistent with a role in cell division, GFP-StkP and its cognate phosphatase, GFP-PhpP, both localize to the division site. StkP localization depends on its penicillin-binding protein and Ser/Thr-associated domains that likely sense uncross-linked peptidoglycan, because StkP and PhpP delocalize in the presence of antibiotics that target the latest stages of cell-wall biosynthesis and in cells that have stopped dividing. Time-lapse microscopy shows that StkP displays an intermediate timing of recruitment to midcell: StkP arrives shortly after FtsA but before DivIVA. Furthermore, StkP remains at midcell longer than FtsA, until division is complete. Cells mutated for stkP are perturbed in cell-wall synthesis and display elongated morphologies with multiple, often unconstricted, FtsA and DivIVA rings. The data show that StkP plays an important role in regulating cell-wall synthesis and controls correct septum progression and closure. Overall, our results indicate that StkP signals information about the cell-wall status to key cell-division proteins and in this way acts as a regulator of cell division.
Links	PubMed PMC3326482 Online version:10.1073/pnas.1119172109
Keywords	Anti-Bacterial Agents/pharmacology; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Cell Division/drug effects; Cell Proliferation/drug effects; Cell Wall/drug effects; Cell Wall/metabolism; Conserved Sequence; Enzyme Activation/drug effects; Extracellular Space/drug effects; Extracellular Space/metabolism; Green Fluorescent Proteins/metabolism; Humans; Ligands; Models, Biological; Phosphorylation/drug effects; Protein Structure, Tertiary; Protein Transport/drug effects; Protein-Serine-Threonine Kinases/chemistry; Protein-Serine-Threonine Kinases/metabolism; Recombinant Fusion Proteins/metabolism; Streptococcus pneumoniae/cytology; Streptococcus pneumoniae/drug effects; Streptococcus pneumoniae/enzymology; Time Factors
edit table

Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
STREE:STKP		GO:0051302: regulation of cell division	ECO:0000315:		P		Figure 6 and figure 7 display unbalanced cell growth and division in the absence of StkP. Wild-type cells were able to develop one cell-division ring in the center while cells with mutated strains became over-elongated and formed multiple rings, leading to division into more than two daughter cells.	complete CACAO 3980
STREE:STKP	involved_in	GO:0051302: regulation of cell division	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

See Help:References for how to manage references in GONUTS.