User:Nzm5146

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BACSU:YCIB	2015-10-14 09:52:53 CDT	GO:0051286 cell tip (C)	PMID:20013255	ECO:0000314 direct assay evidence used in manual assertion	Figure 5A shows a flagged YciB being expressed in Bacillus subtilis and analyzed via immunofluorescence. The arrows within the figure are locating areas with a main concentration of Ycib-FLAG. These locations are near areas of division and cell poles.	challenge
updatedbyinstructor	ECOLI:FTSN	2015-10-19 10:31:40 CDT	GO:0030428 cell septum (C)	PMID:14729705	ECO:0000314 direct assay evidence used in manual assertion	In Figure 1B, wild-type cells of E. coli were fixed and the FtsN protein was visualized via immunofluorescence microscopy. FtsN is shown to be localized at the septum, indicated by the arrows.	challenge
acceptable	BACSU:SMF	2015-10-28 18:46:44 CDT	GO:1990814 DNA/DNA annealing activity (F)	PMID:23779106	ECO:0000314 direct assay evidence used in manual assertion	In Figure 6B, DprA is shown to facilitate the annealing of DNA in the present of single-stranded binding proteins via gel electrophoresis. Both double-stranded and single-stranded DNA are present when DprA is present. Only single-stranded DNA is present in the absence of DprA.	challenge
acceptable	HUMAN:ZN365	2015-10-28 19:09:02 CDT	GO:0005813 centrosome (C)	PMID:16617106	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows localization of Su48 at the centrosome. Fluorescence microscopy was used to visualize Su48 inside the cell.	challenge
acceptable	SYNY3:SEPF	2015-11-11 10:22:46 CST	GO:0030428 cell septum (C)	PMID:19648234	ECO:0000314 direct assay evidence used in manual assertion	Figure 3b (middle image) shows localization of SepF at the cell septum using fluorescence microscopy.	challenge
acceptable	SHIFL:ICSA	2015-11-12 21:01:00 CST	GO:0051286 cell tip (C)	PMID:19684128	ECO:0000314 direct assay evidence used in manual assertion	Figure 4A shows the localization of IcsA at the polar caps, shown by the arrows, in a wild-type strain of S. flexneri. This was detected by indirect immunofluorescence using an antibody to IcsA.	challenge
acceptable	ECOLI:RSEA	2015-11-13 10:35:33 CST	GO:0005886 plasma membrane (C)	PMID:12183369	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows localization of RseA fragments in the inner membrane of E. coli via Western Blot analysis.	challenge
updatedbyinstructor	ECOLI:FEPA	2015-11-13 11:03:14 CST	GO:0030313 cell envelope (C)	PMID:23798405	ECO:0000314 direct assay evidence used in manual assertion	Figure 1A shows FepA localizing at the E. coli cell envelope via fluorescence microscopy.	challenge
updatedbyinstructor	ECOLI:TONB	2015-11-13 11:21:55 CST	GO:0030313 cell envelope (C)	PMID:23798405	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B shows the localization of TonB in the E. coli cell envelope via fluorescence microscopy.	challenge

acceptable:6
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Nzm5146

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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