PMID:20013255

Citation	Bramkamp, M (2010) The putative Bacillus subtilis L,D-transpeptidase YciB is a lipoprotein that localizes to the cell poles in a divisome-dependent manner. Arch. Microbiol. 192:57-68
Abstract	Cell wall synthesis in bacteria is spatially organized by cytoskeletal structures. Common to all cell wall-bearing bacteria, the cytokinetic machinery localizes the cell wall synthesis to the site of septation. Recently, MinJ, a new component of the cytokinetic machinery, or divisome, of Bacillus subtilis has been described. MinJ is part of the division site selection system but also essential for correct assembly of the divisome. Here, I used the isolated PDZ domain of MinJ for co-elution experiments. One of the proteins that co-eluted was the so far uncharacterized, putative L,D-transpeptidase protein YciB. Evidence is shown that YciB localizes to the cell poles. YciB localization depends on the existence of a mature divisome, suggesting that L,D-transpeptidases are, like penicillin-binding proteins, part of the divisome.
Links	PubMed Online version:10.1007/s00203-009-0532-5
Keywords	Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Bacillus subtilis/cytology; Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Bacteria/cytology; Bacteria/genetics; Bacteria/metabolism; Bacterial Outer Membrane Proteins/analysis; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/metabolism; Cell Cycle Proteins/genetics; Cell Cycle Proteins/metabolism; Cell Polarity/genetics; Cell Wall/genetics; Cell Wall/metabolism; DNA, Bacterial/genetics; DNA, Bacterial/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Genes, Bacterial; Lipoproteins/analysis; Lipoproteins/genetics; Lipoproteins/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Mutation; Penicillin-Binding Proteins/genetics; Penicillin-Binding Proteins/metabolism; Peptidyl Transferases/analysis; Peptidyl Transferases/genetics; Peptidyl Transferases/metabolism; Protein Subunits/genetics; Protein Subunits/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/metabolism; Spores, Bacterial/genetics; Spores, Bacterial/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:YCIB		GO:0051286: cell tip	ECO:0000314:		C		Figure 5A shows a flagged YciB being expressed in Bacillus subtilis and analyzed via immunofluorescence. The arrows within the figure are locating areas with a main concentration of Ycib-FLAG. These locations are near areas of division and cell poles.	complete CACAO 11019

References

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