GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:Team PSU 4

From GONUTS
Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptable9BACT:Q1PZD8Rzb5325, Team PSU 42015-11-17 16:35:26 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Results: “In contrast, anti-NXR was very specifically observed at the tubule-like structures in the anammoxosome (Fig. 8) in both transverse (Fig. 8C) and longitudinal (Fig. 8A, B, and D) sectioned tubules.”

challenge
acceptable9BACT:Q1Q0T4Rzb5325, Team PSU 42015-11-17 16:35:16 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig 7 - “K. stuttgartiensis cells incubated with affinity-purified antibodies against HZS. Thin sections were incubated with 1:200-diluted anti-HZS (A to C) or 1:300-diluted anti-HZS (D). Gold particles were visible only inside the anammoxosome. “

challenge
acceptable9BACT:Q1PVE0Rzb5325, Team PSU 42015-11-17 16:34:55 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig 4 - “K. stuttgartiensis cells incubated with antibodies against kustc0457/58. Thin sections were incubated with 1:150-diluted anti-kustc0457/58 (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-kustc0457/58 (D). Gold particles were visible only inside the anammoxosome, preferentially located toward the inner side of the anammoxosome membrane. Incubation with preimmune serum led to the detection of a few gold particles throughout the cell.”

challenge
acceptable9BACT:Q1PZD4Rzb5325, Team PSU 42015-11-17 16:27:16 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Results: “anti-NXR was very specifically observed at the tubule-like structures in the anammoxosome (Fig. 8) in both transverse (Fig. 8C) and longitudinal (Fig. 8A, B, and D) sectioned tubules.”

challenge
acceptable9BACT:Q1Q0T3Rzb5325, Team PSU 42015-11-17 15:35:07 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig 7 -“K. stuttgartiensis cells incubated with affinity-purified antibodies against HZS. Thin sections were incubated with 1:200-diluted anti-HZS (A to C) or 1:300-diluted anti-HZS (D). Gold particles were visible only inside the anammoxosome. “

challenge
acceptable9BACT:Q1PX48Rzb5325, Team PSU 42015-11-17 15:34:45 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig 5- “ Thin sections were incubated with 1:300-diluted anti-HOX (A and B) or 1:100-diluted anti-HOX (C and D). Gold particles were visible only inside the anammoxosome. HOX appeared to be preferentially localized in the proximity of the anammoxosome membrane.”

challenge
acceptable9BACT:Q1PVE1Rzb5325, Team PSU 42015-11-17 15:34:25 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig 4 - "K. stuttgartiensis cells incubated with antibodies against kustc0457/58. Thin sections were incubated with 1:150-diluted anti-kustc0457/58 (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-kustc0457/58 (D). Gold particles were visible only inside the anammoxosome, preferentially located toward the inner side of the anammoxosome membrane. Incubation with preimmune serum led to the detection of a few gold particles throughout the cell.”

challenge
updatedbyinstructor9BACT:Q1Q0T2Rzb5325, Team PSU 42015-11-17 15:33:42 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig 7- “K. stuttgartiensis cells incubated with antibodies against HZS. Thin sections were incubated with 1:100-diluted anti-HZS (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-HZS (D). Gold particles were visible only inside the anammoxosome.”

challenge
acceptable9BACT:Q1PW30Rzb5325, Team PSU 42015-11-17 15:28:47 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Fig. 6 stuttgartiensis cells incubated with antibodies against HDH. Thin sections were incubated with 1:100-diluted anti-HDH (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-HDH (D). Gold particles were visible only inside the anammoxosome"

challenge
acceptable9BACT:Q1PZD5Rzb5325, Team PSU 42015-11-17 15:14:35 CSTGO:0044222 anammoxosome (C)PMID:25962914ECO:0000314 direct assay evidence used in manual assertion

Results: “In contrast, anti-NXR was very specifically observed at the tubule-like structures in the anammoxosome (Fig. 8) in both transverse (Fig. 8C) and longitudinal (Fig. 8A, B, and D) sectioned tubules.”

challenge
updatedbyinstructorECOLI:TONBNzm5146, Team PSU 42015-11-13 11:21:55 CSTGO:0030313 cell envelope (C)PMID:23798405ECO:0000314 direct assay evidence used in manual assertion

Figure 1B shows the localization of TonB in the E. coli cell envelope via fluorescence microscopy.

challenge
updatedbyinstructorECOLI:FEPANzm5146, Team PSU 42015-11-13 11:03:14 CSTGO:0030313 cell envelope (C)PMID:23798405ECO:0000314 direct assay evidence used in manual assertion

Figure 1A shows FepA localizing at the E. coli cell envelope via fluorescence microscopy.

challenge
acceptableECOLI:RSEANzm5146, Team PSU 42015-11-13 10:35:33 CSTGO:0005886 plasma membrane (C)PMID:12183369ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows localization of RseA fragments in the inner membrane of E. coli via Western Blot analysis.

challenge
acceptableSHIFL:ICSANzm5146, Team PSU 42015-11-12 21:01:00 CSTGO:0051286 cell tip (C)PMID:19684128ECO:0000314 direct assay evidence used in manual assertion

Figure 4A shows the localization of IcsA at the polar caps, shown by the arrows, in a wild-type strain of S. flexneri. This was detected by indirect immunofluorescence using an antibody to IcsA.

challenge
acceptableSYNY3:SEPFNzm5146, Team PSU 42015-11-11 10:22:46 CSTGO:0030428 cell septum (C)PMID:19648234ECO:0000314 direct assay evidence used in manual assertion

Figure 3b (middle image) shows localization of SepF at the cell septum using fluorescence microscopy.

challenge
acceptableHUMAN:ZN365Nzm5146, Team PSU 42015-10-28 19:09:02 CDTGO:0005813 centrosome (C)PMID:16617106ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows localization of Su48 at the centrosome. Fluorescence microscopy was used to visualize Su48 inside the cell.

challenge
acceptableBACSU:SMFNzm5146, Team PSU 42015-10-28 18:46:44 CDTGO:1990814 DNA/DNA annealing activity (F)PMID:23779106ECO:0000314 direct assay evidence used in manual assertion

In Figure 6B, DprA is shown to facilitate the annealing of DNA in the present of single-stranded binding proteins via gel electrophoresis. Both double-stranded and single-stranded DNA are present when DprA is present. Only single-stranded DNA is present in the absence of DprA.

challenge
updatedbyinstructorECOLI:FTSNNzm5146, Team PSU 42015-10-19 10:31:40 CDTGO:0030428 cell septum (C)PMID:14729705ECO:0000314 direct assay evidence used in manual assertion

In Figure 1B, wild-type cells of E. coli were fixed and the FtsN protein was visualized via immunofluorescence microscopy. FtsN is shown to be localized at the septum, indicated by the arrows.

challenge
acceptableBACSU:YCIBNzm5146, Team PSU 42015-10-14 09:52:53 CDTGO:0051286 cell tip (C)PMID:20013255ECO:0000314 direct assay evidence used in manual assertion

Figure 5A shows a flagged YciB being expressed in Bacillus subtilis and analyzed via immunofluorescence. The arrows within the figure are locating areas with a main concentration of Ycib-FLAG. These locations are near areas of division and cell poles.

challenge

What do the icons mean in the status column?

assessment icons.jpg

Pages in category "Team PSU 4"

The following 4 pages are in this category, out of 4 total.


Jump to pages starting with: A K N R