acceptable | 9BACT:Q1PZD8 | Rzb5325, Team PSU 4 | 2015-11-17 16:35:26 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Results: “In contrast, anti-NXR was very specifically observed at the tubule-like structures in the anammoxosome (Fig. 8) in both transverse (Fig. 8C) and longitudinal (Fig. 8A, B, and D) sectioned tubules.”
| challenge |
acceptable | 9BACT:Q1Q0T4 | Rzb5325, Team PSU 4 | 2015-11-17 16:35:16 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig 7 - “K. stuttgartiensis cells incubated with affinity-purified antibodies against HZS. Thin sections were incubated with 1:200-diluted anti-HZS (A to C) or 1:300-diluted anti-HZS (D). Gold particles were visible only inside the anammoxosome. “
| challenge |
acceptable | 9BACT:Q1PVE0 | Rzb5325, Team PSU 4 | 2015-11-17 16:34:55 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig 4 - “K. stuttgartiensis cells incubated with antibodies against kustc0457/58. Thin sections were incubated with 1:150-diluted anti-kustc0457/58 (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-kustc0457/58 (D). Gold particles were visible only inside the anammoxosome, preferentially located toward the inner side of the anammoxosome membrane. Incubation with preimmune serum led to the detection of a few gold particles throughout the cell.”
| challenge |
acceptable | 9BACT:Q1PZD4 | Rzb5325, Team PSU 4 | 2015-11-17 16:27:16 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Results: “anti-NXR was very specifically observed at the tubule-like structures in the anammoxosome (Fig. 8) in both transverse (Fig. 8C) and longitudinal (Fig. 8A, B, and D) sectioned tubules.”
| challenge |
acceptable | 9BACT:Q1Q0T3 | Rzb5325, Team PSU 4 | 2015-11-17 15:35:07 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig 7 -“K. stuttgartiensis cells incubated with affinity-purified antibodies against HZS. Thin sections were incubated with 1:200-diluted anti-HZS (A to C) or 1:300-diluted anti-HZS (D). Gold particles were visible only inside the anammoxosome. “
| challenge |
acceptable | 9BACT:Q1PX48 | Rzb5325, Team PSU 4 | 2015-11-17 15:34:45 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig 5- “ Thin sections were incubated with 1:300-diluted anti-HOX (A and B) or 1:100-diluted anti-HOX (C and D). Gold particles were visible only inside the anammoxosome. HOX appeared to be preferentially localized in the proximity of the anammoxosome membrane.”
| challenge |
acceptable | 9BACT:Q1PVE1 | Rzb5325, Team PSU 4 | 2015-11-17 15:34:25 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig 4 - "K. stuttgartiensis cells incubated with antibodies against kustc0457/58. Thin sections were incubated with 1:150-diluted anti-kustc0457/58 (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-kustc0457/58 (D). Gold particles were visible only inside the anammoxosome, preferentially located toward the inner side of the anammoxosome membrane. Incubation with preimmune serum led to the detection of a few gold particles throughout the cell.”
| challenge |
updatedbyinstructor | 9BACT:Q1Q0T2 | Rzb5325, Team PSU 4 | 2015-11-17 15:33:42 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig 7- “K. stuttgartiensis cells incubated with antibodies against HZS. Thin sections were incubated with 1:100-diluted anti-HZS (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-HZS (D). Gold particles were visible only inside the anammoxosome.”
| challenge |
acceptable | 9BACT:Q1PW30 | Rzb5325, Team PSU 4 | 2015-11-17 15:28:47 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Fig. 6 stuttgartiensis cells incubated with antibodies against HDH. Thin sections were incubated with 1:100-diluted anti-HDH (A and B), 1:50-diluted preimmune serum (C), or 1:100-diluted anti-HDH (D). Gold particles were visible only inside the anammoxosome"
| challenge |
acceptable | 9BACT:Q1PZD5 | Rzb5325, Team PSU 4 | 2015-11-17 15:14:35 CST | GO:0044222 anammoxosome (C) | PMID:25962914 | ECO:0000314 direct assay evidence used in manual assertion | Results: “In contrast, anti-NXR was very specifically observed at the tubule-like structures in the anammoxosome (Fig. 8) in both transverse (Fig. 8C) and longitudinal (Fig. 8A, B, and D) sectioned tubules.”
| challenge |
updatedbyinstructor | ECOLI:TONB | Nzm5146, Team PSU 4 | 2015-11-13 11:21:55 CST | GO:0030313 cell envelope (C) | PMID:23798405 | ECO:0000314 direct assay evidence used in manual assertion | Figure 1B shows the localization of TonB in the E. coli cell envelope via fluorescence microscopy.
| challenge |
updatedbyinstructor | ECOLI:FEPA | Nzm5146, Team PSU 4 | 2015-11-13 11:03:14 CST | GO:0030313 cell envelope (C) | PMID:23798405 | ECO:0000314 direct assay evidence used in manual assertion | Figure 1A shows FepA localizing at the E. coli cell envelope via fluorescence microscopy.
| challenge |
acceptable | ECOLI:RSEA | Nzm5146, Team PSU 4 | 2015-11-13 10:35:33 CST | GO:0005886 plasma membrane (C) | PMID:12183369 | ECO:0000314 direct assay evidence used in manual assertion | Figure 5 shows localization of RseA fragments in the inner membrane of E. coli via Western Blot analysis.
| challenge |
acceptable | SHIFL:ICSA | Nzm5146, Team PSU 4 | 2015-11-12 21:01:00 CST | GO:0051286 cell tip (C) | PMID:19684128 | ECO:0000314 direct assay evidence used in manual assertion | Figure 4A shows the localization of IcsA at the polar caps, shown by the arrows, in a wild-type strain of S. flexneri. This was detected by indirect immunofluorescence using an antibody to IcsA.
| challenge |
acceptable | SYNY3:SEPF | Nzm5146, Team PSU 4 | 2015-11-11 10:22:46 CST | GO:0030428 cell septum (C) | PMID:19648234 | ECO:0000314 direct assay evidence used in manual assertion | Figure 3b (middle image) shows localization of SepF at the cell septum using fluorescence microscopy.
| challenge |
acceptable | HUMAN:ZN365 | Nzm5146, Team PSU 4 | 2015-10-28 19:09:02 CDT | GO:0005813 centrosome (C) | PMID:16617106 | ECO:0000314 direct assay evidence used in manual assertion | Figure 2 shows localization of Su48 at the centrosome. Fluorescence microscopy was used to visualize Su48 inside the cell.
| challenge |
acceptable | BACSU:SMF | Nzm5146, Team PSU 4 | 2015-10-28 18:46:44 CDT | GO:1990814 DNA/DNA annealing activity (F) | PMID:23779106 | ECO:0000314 direct assay evidence used in manual assertion | In Figure 6B, DprA is shown to facilitate the annealing of DNA in the present of single-stranded binding proteins via gel electrophoresis. Both double-stranded and single-stranded DNA are present when DprA is present. Only single-stranded DNA is present in the absence of DprA.
| challenge |
updatedbyinstructor | ECOLI:FTSN | Nzm5146, Team PSU 4 | 2015-10-19 10:31:40 CDT | GO:0030428 cell septum (C) | PMID:14729705 | ECO:0000314 direct assay evidence used in manual assertion | In Figure 1B, wild-type cells of E. coli were fixed and the FtsN protein was visualized via immunofluorescence microscopy. FtsN is shown to be localized at the septum, indicated by the arrows.
| challenge |
acceptable | BACSU:YCIB | Nzm5146, Team PSU 4 | 2015-10-14 09:52:53 CDT | GO:0051286 cell tip (C) | PMID:20013255 | ECO:0000314 direct assay evidence used in manual assertion | Figure 5A shows a flagged YciB being expressed in Bacillus subtilis and analyzed via immunofluorescence. The arrows within the figure are locating areas with a main concentration of Ycib-FLAG. These locations are near areas of division and cell poles.
| challenge |