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User:Ksylvester

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorYEAST:SNU662013-09-15 17:51:46 CDTGO:0005634 nucleus (C)PMID:15620657ECO:0000314 direct assay evidence used in manual assertion

Figure S4 of the supplementary data indicates nuclear localization of Snu66 in S. cerevisiae.

challenge
unacceptable?2013-09-15 17:55:05 CDTGO:0048024 regulation of mRNA splicing, via spliceosome (Figure 3A. loss of HUB1 activity reduces splicing in pus1, sec13, rpa43, and rad24 of Saccharomyces cerevisiae)PMID:15620657IMP: Inferred from Mutant Phenotype
challenge
acceptableYEAST:CTR12013-09-24 20:51:26 CDTGO:0016021 integral to membrane (C)PMID:8293472ECO:0000314 direct assay evidence used in manual assertion

Figure 3 shows an immunoelectron microscope picture of epitope tagged protein that is located in the membrane where the C-terminus side is inside the cell.

challenge
acceptableECOLI:SECY2013-11-10 17:06:31 CSTGO:0005887 integral to plasma membrane (C)PMID:2538820ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows PrlA (SecY) is enriched in the plasma membrane by immunoblotting using anti-peptide antisera

challenge

acceptable:2
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Ksylvester

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Ksylvester

What do the icons mean in the status column?

assessment icons.jpg