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PMID:8293472
| Citation |
Dancis, A, Yuan, DS, Haile, D, Askwith, C, Eide, D, Moehle, C, Kaplan, J and Klausner, RD (1994) Molecular characterization of a copper transport protein in S. cerevisiae: an unexpected role for copper in iron transport. Cell 76:393-402 |
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| Abstract |
We report the identification and characterization of CTR1, a gene in the yeast S. cerevisiae that encodes a multispanning plasma membrane protein specifically required for high affinity copper transport into the cell. The predicted protein contains a methionine- and serine-rich domain that includes 11 examples of the sequence Met-X2-Met, a motif noted in proteins involved in bacterial copper metabolism. CTR1 mutants and deletion strains have profound deficiency in ferrous iron uptake, thus revealing a requirement for copper in mediating ferrous transport into the cell. Genetic evidence suggests that the target for this requirement is the FET3 gene (detailed in a companion study), predicted to encode a copper-containing protein that acts as a cytosolic ferro-oxidase. These findings provide an unexpected mechanistic link between the uptake of copper and iron. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Biological Transport; Cation Transport Proteins; Cloning, Molecular; Copper/metabolism; Ferrous Compounds/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; Genes, Fungal; Iron/metabolism; Membrane Proteins/genetics; Membrane Proteins/metabolism; Molecular Sequence Data; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Homology, Amino Acid |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0016021: integral component of membrane |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0016021: integral to membrane |
ECO:0000314: |
C |
Figure 3 shows an immunoelectron microscope picture of epitope tagged protein that is located in the membrane where the C-terminus side is inside the cell. |
complete | ||||
See also
References
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