ECOLI:SECY

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	secY (synonyms: prlA)
Protein Name(s)	Protein translocase subunit SecY
External Links
UniProt	P0AGA2
EMBL	X01563 U18997 U00096 AP009048
PIR	A04473
RefSeq	NP_417759.1 YP_492132.1
PDB	2AKH 2AKI 3J01 3J45 3J46
PDBsum	2AKH 2AKI 3J01 3J45 3J46
ProteinModelPortal	P0AGA2
SMR	P0AGA2
DIP	DIP-59302N
IntAct	P0AGA2
STRING	511145.b3300
TCDB	3.A.5.1.1
PaxDb	P0AGA2
PRIDE	P0AGA2
EnsemblBacteria	AAC76325 BAE77991
GeneID	12934410 947799
KEGG	ecj:Y75_p3876 eco:b3300
PATRIC	32122032
EchoBASE	EB0759
EcoGene	EG10766
eggNOG	COG0201
HOGENOM	HOG000080585
InParanoid	P0AGA2
KO	K03076
OMA	QTYVISQ
OrthoDB	EOG651SWP
PhylomeDB	P0AGA2
BioCyc	EcoCyc:SECY ECOL316407:JW3262-MONOMER
EvolutionaryTrace	P0AGA2
PRO	PR:P0AGA2
Proteomes	UP000000318 UP000000625
Genevestigator	P0AGA2
GO	GO:0016021 GO:0005887 GO:0005622 GO:0005886 GO:0065002 GO:0006886 GO:0006605 GO:0043952
Gene3D	1.10.3370.10
HAMAP	MF_01465
InterPro	IPR026593 IPR002208 IPR023201
PANTHER	PTHR10906
Pfam	PF00344
PIRSF	PIRSF004557
SUPFAM	SSF103491
TIGRFAMs	TIGR00967
PROSITE	PS00755 PS00756

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0005887	integral to plasma membrane	PMID:2538820^[1]	ECO:0000314			C	Figure 2 shows PrlA (SecY) is enriched in the plasma membrane by immunoblotting using anti-peptide antisera	complete CACAO 9183
part_of	GO:0005887	integral component of plasma membrane	PMID:2538820^[1]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0031522	cell envelope Sec protein transport complex	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10766 PANTHER:PTN000097337	C	Seeded From UniProt	complete
enables	GO:0008320	protein transmembrane transporter activity	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10766 PANTHER:PTN000097217	F	Seeded From UniProt	complete
involved_in	GO:0006616	SRP-dependent cotranslational protein targeting to membrane, translocation	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10766 PANTHER:PTN000097217 SGD:S000004370	P	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10766 PANTHER:PTN000097337	C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10766 PANTHER:PTN000097337	C	Seeded From UniProt	complete
enables	GO:0005048	signal sequence binding	PMID:21873635^[2]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	EcoGene:EG10766 PANTHER:PTN000097337	F	Seeded From UniProt	complete
involved_in	GO:0065002	intracellular protein transmembrane transport	PMID:2254269^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0043952	protein transport by the Sec complex	PMID:2254269^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	PMID:3004955^[4]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0006886	intracellular protein transport	PMID:2254269^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:3004955^[4]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0065002	intracellular protein transmembrane transport	PMID:7650029^[5]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0065002	intracellular protein transmembrane transport	PMID:3017993^[6]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0065002	intracellular protein transmembrane transport	PMID:2139227^[7]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0043952	protein transport by the Sec complex	PMID:7650029^[5]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0043952	protein transport by the Sec complex	PMID:2167176^[8]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0043952	protein transport by the Sec complex	PMID:1531482^[9]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0032978	protein insertion into membrane from inner side	PMID:1447197^[10]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0031522	cell envelope Sec protein transport complex	PMID:2167176^[8]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0031522	cell envelope Sec protein transport complex	PMID:1531482^[9]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0008320	protein transmembrane transporter activity	PMID:7650029^[5]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008320	protein transmembrane transporter activity	PMID:3017993^[6]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008320	protein transmembrane transporter activity	PMID:2167176^[8]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008320	protein transmembrane transporter activity	PMID:2139227^[7]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0008320	protein transmembrane transporter activity	PMID:1531482^[9]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0006616	SRP-dependent cotranslational protein targeting to membrane, translocation	PMID:9564033^[11]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006616	SRP-dependent cotranslational protein targeting to membrane, translocation	PMID:10397756^[12]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:3004955^[4]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:2538820^[1]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:7650029^[5]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:17309111^[13]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	PMID:15919996^[14]	ECO:0000314	direct assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0005048	signal sequence binding	PMID:15476412^[15]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0015031	protein transport	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002208	P	Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002208	C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000054593	C	Seeded From UniProt	complete
involved_in	GO:0006605	protein targeting	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000054593	P	Seeded From UniProt	complete
involved_in	GO:0043952	protein transport by the Sec complex	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000054593	P	Seeded From UniProt	complete
involved_in	GO:0065002	intracellular protein transmembrane transport	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000054593	P	Seeded From UniProt	complete
part_of	GO:0005887	integral component of plasma membrane	PMID:2202723^[16]	ECO:0000304	author statement supported by traceable reference used in manual assertion		C	Seeded From UniProt	complete
involved_in	GO:0015031	protein transport	GO_REF:0000037 GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0811 UniProtKB-KW:KW-0653	P	Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C	Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	GO_REF:0000037 GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1003 UniProtKB-KW:KW-0997 UniProtKB-SubCell:SL-0037	C	Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 Watanabe, M & Blobel, G (1989) Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins. Proc. Natl. Acad. Sci. U.S.A. 86 1895-9 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 Baba, T et al. (1990) Characterization of cold-sensitive secY mutants of Escherichia coli. J. Bacteriol. 172 7005-10 PubMed GONUTS page
↑ ^4.0 ^4.1 ^4.2 Akiyama, Y & Ito, K (1985) The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins. EMBO J. 4 3351-6 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 ^5.3 Douville, K et al. (1995) SecYEG and SecA are the stoichiometric components of preprotein translocase. J. Biol. Chem. 270 20106-11 PubMed GONUTS page
↑ ^6.0 ^6.1 Bacallao, R et al. (1986) The secY protein can act post-translationally to promote bacterial protein export. J. Biol. Chem. 261 12907-10 PubMed GONUTS page
↑ ^7.0 ^7.1 Driessen, AJ & Wickner, W (1990) Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 87 3107-11 PubMed GONUTS page
↑ ^8.0 ^8.1 ^8.2 Brundage, L et al. (1990) The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell 62 649-57 PubMed GONUTS page
↑ ^9.0 ^9.1 ^9.2 Brundage, L et al. (1992) SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase. J. Biol. Chem. 267 4166-70 PubMed GONUTS page
↑ Werner, PK et al. (1992) Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function. J. Biol. Chem. 267 24523-32 PubMed GONUTS page
↑ Valent, QA et al. (1998) The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J. 17 2504-12 PubMed GONUTS page
↑ Koch, HG et al. (1999) In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli. Mol. Biol. Cell 10 2163-73 PubMed GONUTS page
↑ Zhang, N et al. (2007) Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS. Proteomics 7 484-93 PubMed GONUTS page
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed GONUTS page
↑ Wang, L et al. (2004) Demonstration of a specific Escherichia coli SecY-signal peptide interaction. Biochemistry 43 13185-92 PubMed GONUTS page
↑ Ito, K (1990) Structure, function, and biogenesis of SecY, an integral membrane protein involved in protein export. J. Bioenerg. Biomembr. 22 353-67 PubMed GONUTS page

[PMID:2538820-1] 1.0 ^1.1 ^1.2 Watanabe, M & Blobel, G (1989) Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins. Proc. Natl. Acad. Sci. U.S.A. 86 1895-9 PubMed GONUTS page

[PMID:21873635-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:2254269-3] 3.0 ^3.1 ^3.2 Baba, T et al. (1990) Characterization of cold-sensitive secY mutants of Escherichia coli. J. Bacteriol. 172 7005-10 PubMed GONUTS page

[PMID:3004955-4] 4.0 ^4.1 ^4.2 Akiyama, Y & Ito, K (1985) The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins. EMBO J. 4 3351-6 PubMed GONUTS page

[PMID:7650029-5] 5.0 ^5.1 ^5.2 ^5.3 Douville, K et al. (1995) SecYEG and SecA are the stoichiometric components of preprotein translocase. J. Biol. Chem. 270 20106-11 PubMed GONUTS page

[PMID:3017993-6] 6.0 ^6.1 Bacallao, R et al. (1986) The secY protein can act post-translationally to promote bacterial protein export. J. Biol. Chem. 261 12907-10 PubMed GONUTS page

[PMID:2139227-7] 7.0 ^7.1 Driessen, AJ & Wickner, W (1990) Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 87 3107-11 PubMed GONUTS page

[PMID:2167176-8] 8.0 ^8.1 ^8.2 Brundage, L et al. (1990) The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell 62 649-57 PubMed GONUTS page

[PMID:1531482-9] 9.0 ^9.1 ^9.2 Brundage, L et al. (1992) SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase. J. Biol. Chem. 267 4166-70 PubMed GONUTS page

[PMID:1447197-10] Werner, PK et al. (1992) Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function. J. Biol. Chem. 267 24523-32 PubMed GONUTS page

[PMID:9564033-11] Valent, QA et al. (1998) The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J. 17 2504-12 PubMed GONUTS page

[PMID:10397756-12] Koch, HG et al. (1999) In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli. Mol. Biol. Cell 10 2163-73 PubMed GONUTS page

[PMID:17309111-13] Zhang, N et al. (2007) Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS. Proteomics 7 484-93 PubMed GONUTS page

[PMID:15919996-14] Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed GONUTS page

[PMID:15476412-15] Wang, L et al. (2004) Demonstration of a specific Escherichia coli SecY-signal peptide interaction. Biochemistry 43 13185-92 PubMed GONUTS page

[PMID:2202723-16] Ito, K (1990) Structure, function, and biogenesis of SecY, an integral membrane protein involved in protein export. J. Bioenerg. Biomembr. 22 353-67 PubMed GONUTS page

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ECOLI:SECY

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