User:HPatel

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BPSPP:TUBE	2018-04-01 08:43:41 CDT	GO:0098005 viral head-tail joining (P)	PMID:24443902	ECO:0000314 direct assay evidence used in manual assertion	An in-vitro complementation assay was used to study incorporation of gp17 during assembly of viral particles. Each extract was separately mixed with 4 different components; water, complete tails purified from SPP1sus31 with gp17, tails without gp17 purified from SPP1sus82, and purified gp17. After incubation, the titer of the progeny was calculated in Figure 7. The expected result was sus31 tails in both extracts allowed it to join to the capsids for virion assembly. When mixing SPP1sus82 tails without gp17 with SPP1sus45, virion particles assembled, suggesting endogenous gp17 was able to join head and tail. SPP1sus82 had a low titer, thus tails weren’t able to attach. Finally, purified gp17 mixed SPP1sus82 extracts was sufficient for tail to head stickage. This assay shows that gp17 is involved in binding to the tail and later attaching to the DNA-filled capsid.	challenge
acceptable	BPT4:SEGB	2018-04-12 15:47:35 CDT	GO:0004520 endodeoxyribonuclease activity (F)	PMID:18281701	ECO:0000314 direct assay evidence used in manual assertion	The T4 SegB protein is a site-specific endonuclease that recognizes a 27-bp sequence. 666 bp segB ORF was placed under the control of T7 promoter in pET-15b vector. SegB protein was overproduced in E. coli T7 lac promoter system and purified. The purified protein was incubated with a linear plasmid DNA and products were analyzed of the reaction through gel electrophoresis (Figure 3). SegB cleaved DNA by introducing double-strand breaks in reactions requiring Mg2+, Mn2+, or Ca2+ cations. With Mg2+ and Ca2+ cation, SegB cleaved the DNA at 1 point, creating 2.2 kb and 1.6 kb fragments. In the presence of Mn2+, multiple cleavage sites were present.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by HPatel

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	RPatel, Team Green B 2018	ENTFA:Q837U3	GO:0070051 - fibrinogen binding (F)	PMID:29487730	ECO:0000021 physical interaction evidence	challenge	C: 1
acceptable	HPaudel, Team Green B 2018	BPT5:TMP	GO:0061783 - peptidoglycan muralytic activity (F)	PMID:18348984	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3

0 annotations fixed by HPatel

What do the icons mean in the status column?