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BPSPP:TUBE
Contents
| Species (Taxon ID) | Bacillus phage SPP1 (Bacteriophage SPP1). (10724) | |
| Gene Name(s) | 17.1 | |
| Protein Name(s) | Tail tube protein gp17.1* (ECO:0000303 with PMID:17611601[1])
TTP (ECO:0000305) Gene product 17.1 (ECO:0000305) gp17.1 (ECO:0000305) Major tail protein (ECO:0000303 with PMID:18786146[2]) MTP (ECO:0000303 with PMID:18786146[2]) | |
| External Links | ||
| UniProt | O48449 | |
| EMBL | X97918 | |
| PIR | T42289 | |
| RefSeq | NP_690680.1 YP_710298.1 | |
| PDB | 5A20 5A21 | |
| PDBsum | 5A20 5A21 | |
| ProteinModelPortal | O48449 | |
| SMR | O48449 | |
| GeneID | 4229189 955320 | |
| KEGG | vg:4229189 vg:955320 | |
| OrthoDB | VOG090000SU | |
| Proteomes | UP000002559 | |
| GO | GO:0019012 GO:0098026 GO:0099001 | |
| CDD | cd00063 | |
| Gene3D | 2.60.40.10 | |
| InterPro | IPR003961 IPR036116 IPR013783 IPR011855 | |
| Pfam | PF00041 PF06199 | |
| SMART | SM00060 | |
| SUPFAM | SSF49265 | |
| TIGRFAMs | TIGR02126 | |
| PROSITE | PS50853 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0098026 |
virus tail, tube |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0098026 |
virus tail, tube |
ECO:0000314 |
C |
TTP, gp17.1 has been identified to be able to self assemble into long tubes in the absence of other phage proteins. In figure 6 there is a comparison done between gp17.1 and tails of SSP1 virions that show similar morphology. These samples were negatively stained and it was observed that both gp17.1 is hollow and the empty virion tails that have ejected their DNA after incubation in a SSP1 bacterial receptor. Aligned segments of gp17.1 were taken then calculation was performed for its diffraction pattern. This pattern revealed that tubes have a 6-fold symmetry which are made by stacks of hexameric rings. These rings form helical tubes that elongate. |
complete | |||||
|
part_of |
GO:0019012 |
virion |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0098005 |
viral head-tail joining |
ECO:0000314 |
P |
An in-vitro complementation assay was used to study incorporation of gp17 during assembly of viral particles. Each extract was separately mixed with 4 different components; water, complete tails purified from SPP1sus31 with gp17, tails without gp17 purified from SPP1sus82, and purified gp17. After incubation, the titer of the progeny was calculated in Figure 7. The expected result was sus31 tails in both extracts allowed it to join to the capsids for virion assembly. When mixing SPP1sus82 tails without gp17 with SPP1sus45, virion particles assembled, suggesting endogenous gp17 was able to join head and tail. SPP1sus82 had a low titer, thus tails weren’t able to attach. Finally, purified gp17 mixed SPP1sus82 extracts was sufficient for tail to head stickage. This assay shows that gp17 is involved in binding to the tail and later attaching to the DNA-filled capsid. |
complete | |||||
|
part_of |
GO:0098026 |
virus tail, tube |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0046718 |
viral entry into host cell |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0099001 |
viral genome ejection through host cell envelope, long flexible tail mechanism |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019012 |
virion |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0098015 |
virus tail |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Plisson, C et al. (2007) Structure of bacteriophage SPP1 tail reveals trigger for DNA ejection. EMBO J. 26 3720-8 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Auzat, I et al. (2008) Origin and function of the two major tail proteins of bacteriophage SPP1. Mol. Microbiol. 70 557-69 PubMed GONUTS page
- ↑ Langlois, C et al. (2015) Bacteriophage SPP1 tail tube protein self-assembles into β-structure-rich tubes. J. Biol. Chem. 290 3836-49 PubMed GONUTS page
- ↑ Vinga, I et al. (2006) The minor capsid protein gp7 of bacteriophage SPP1 is required for efficient infection of Bacillus subtilis. Mol. Microbiol. 61 1609-21 PubMed GONUTS page
- ↑ Auzat, I et al. (2014) A touch of glue to complete bacteriophage assembly: the tail-to-head joining protein (THJP) family. Mol. Microbiol. 91 1164-78 PubMed GONUTS page
