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PMID:18786146

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Citation

Auzat, I, Dröge, A, Weise, F, Lurz, R and Tavares, P (2008) Origin and function of the two major tail proteins of bacteriophage SPP1. Mol. Microbiol. 70:557-69

Abstract

The majority of bacteriophages have a long non-contractile tail (Siphoviridae) that serves as a conduit for viral DNA traffic from the phage capsid to the host cell at the beginning of infection. The 160-nm-long tail tube of Bacillus subtilis bacteriophage SPP1 is shown to be composed of two major tail proteins (MTPs), gp17.1 and gp17.1*, at a ratio of about 3:1. They share a common amino-terminus, but the latter species has approximately 10 kDa more than gp17.1. A CCC.UAA sequence with overlapping proline codons at the 3' end of gene 17.1 drives a programmed translational frameshift to another open reading frame. The recoding event generates gp17.1*. Phages carrying exclusively gp17.1 or gp17.1* are viable, but tails are structurally distinct. gp17.1 and the carboxyl-terminus of gp17.1* have a distinct evolutionary history correlating with different functions: the polypeptide sequence identical in the two proteins is responsible for assembly of the tail tube while the additional module of gp17.1* shields the structure exterior exposed to the environment. The carboxyl-terminal extension is an elaboration present in some tailed bacteriophages. Different extensions were found to combine in a mosaic fashion with the MTP essential module in a subset of Siphoviridae genomes.

Links

PubMed Online version:10.1111/j.1365-2958.2008.06435.x

Keywords

Bacillus Phages/genetics; Bacillus Phages/physiology; Bacillus subtilis/virology; DNA, Viral/genetics; Escherichia coli/genetics; Evolution, Molecular; Frameshifting, Ribosomal; Genes, Viral; Genome, Viral; Mutagenesis, Site-Directed; Mutation; Nucleic Acid Conformation; Open Reading Frames; Plasmids; Sequence Homology, Amino Acid; Viral Tail Proteins/genetics; Virus Replication/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPSPP:TUBE

located_in

GO:0098026: virus tail, tube

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPSPP:GP171

part_of

GO:0098026: virus tail, tube

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPSPP:TUBE

part_of

GO:0098026: virus tail, tube

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete


See also

References

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