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Cacao

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GO:0004519endonuclease activityPMID:18281701IDA: Inferred from Direct Assay F
This annotation made on page: BPT4:SEGB
By: HPatel (group Team Blue B 2018) on 2018-04-12 15:47:35 CDT.



TeamPoints
Team Blue B 20185




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment		Ivanerill2018-05-07 08:10:37 CDTNo notes given.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-05-02 11:40:47 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004520 endodeoxyribonuclease activity PMID:18281701 IDA: Inferred from Direct Assay F The T4 SegB protein is a site-specific endonuclease that recognizes a 27-bp sequence. 666 bp segB ORF was placed under the control of T7 promoter in pET-15b vector. SegB protein was overproduced in E. coli T7 lac promoter system and purified. The purified protein was incubated with a linear plasmid DNA and products were analyzed of the reaction through gel electrophoresis (Figure 3). SegB cleaved DNA by introducing double-strand breaks in reactions requiring Mg2+, Mn2+, or Ca2+ cations. With Mg2+ and Ca2+ cation, SegB cleaved the DNA at 1 point, creating 2.2 kb and 1.6 kb fragments. In the presence of Mn2+, multiple cleavage sites were present. complete
CACAO 13238
on BPT4:SEGB
Flagged
Public
Assessment		Ivanerill2018-04-30 05:13:42 CDT

The comment was "there is a more specific GO term available". This typically means looking at your GO term (a molecular activity term) and seeing if there are more specific terms (children) that apply. Not changing it to a biological process term. I don't believe the paper provides support for such an specific claim.

Requires Changes
✗ Go term
Public
Assessment		DanielRenfro2018-04-22 21:04:00 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0090305 endonuclease activity PMID:18281701 IDA: Inferred from Direct Assay F The T4 SegB protein is a site-specific endonuclease that recognizes a 27-bp sequence. 666 bp segB ORF was placed under the control of T7 promoter in pET-15b vector. SegB protein was overproduced in E. coli T7 lac promoter system and purified. The purified protein was incubated with a linear plasmid DNA and products were analyzed of the reaction through gel electrophoresis (Figure 3). SegB cleaved DNA by introducing double-strand breaks in reactions requiring Mg2+, Mn2+, or Ca2+ cations. With Mg2+ and Ca2+ cation, SegB cleaved the DNA at 1 point, creating 2.2 kb and 1.6 kb fragments. In the presence of Mn2+, multiple cleavage sites were present. complete
CACAO 13238
on BPT4:SEGB
Flagged
Public
Assessment		Ivanerill2018-04-15 15:26:48 CDT

There is a more specific GO term available.

Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original


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