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BPT4:SEGB
Contents
Species (Taxon ID) | Enterobacteria phage T4 (Bacteriophage T4). (10665) | |
Gene Name(s) | segB (synonyms: trnA.1) | |
Protein Name(s) | Putative endonuclease segB
Endodeoxyribonuclease segB | |
External Links | ||
UniProt | P13325 | |
EMBL | X03016 Z69338 AF158101 | |
RefSeq | NP_049745.2 | |
GeneID | 1258659 | |
KEGG | vg:1258659 | |
OrthoDB | VOG090000QM | |
Proteomes | UP000009087 | |
GO | GO:0004519 | |
Gene3D | 3.40.1440.10 | |
InterPro | IPR000305 IPR035901 | |
Pfam | PF01541 | |
SMART | SM00465 | |
SUPFAM | SSF82771 | |
PROSITE | PS50164 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0004520 |
endodeoxyribonuclease activity |
ECO:0000314 |
F |
The T4 SegB protein is a site-specific endonuclease that recognizes a 27-bp sequence. 666 bp segB ORF was placed under the control of T7 promoter in pET-15b vector. SegB protein was overproduced in E. coli T7 lac promoter system and purified. The purified protein was incubated with a linear plasmid DNA and products were analyzed of the reaction through gel electrophoresis (Figure 3). SegB cleaved DNA by introducing double-strand breaks in reactions requiring Mg2+, Mn2+, or Ca2+ cations. With Mg2+ and Ca2+ cation, SegB cleaved the DNA at 1 point, creating 2.2 kb and 1.6 kb fragments. In the presence of Mn2+, multiple cleavage sites were present. |
complete | |||||
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004519 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004518 |
P |
Seeded From UniProt |
complete | ||
enables |
GO:0004519 |
endonuclease activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0004518 |
nuclease activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Brok-Volchanskaya, VS et al. (2008) Phage T4 SegB protein is a homing endonuclease required for the preferred inheritance of T4 tRNA gene region occurring in co-infection with a related phage. Nucleic Acids Res. 36 2094-105 PubMed GONUTS page
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