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BPT4:SEGB

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Species (Taxon ID) Enterobacteria phage T4 (Bacteriophage T4). (10665)
Gene Name(s) segB (synonyms: trnA.1)
Protein Name(s) Putative endonuclease segB

Endodeoxyribonuclease segB

External Links
UniProt P13325
EMBL X03016
Z69338
AF158101
RefSeq NP_049745.2
GeneID 1258659
KEGG vg:1258659
OrthoDB VOG090000QM
Proteomes UP000009087
GO GO:0004519
Gene3D 3.40.1440.10
InterPro IPR000305
IPR035901
Pfam PF01541
SMART SM00465
SUPFAM SSF82771
PROSITE PS50164

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004520

endodeoxyribonuclease activity

PMID:18281701[1]

ECO:0000314

F

The T4 SegB protein is a site-specific endonuclease that recognizes a 27-bp sequence. 666 bp segB ORF was placed under the control of T7 promoter in pET-15b vector. SegB protein was overproduced in E. coli T7 lac promoter system and purified. The purified protein was incubated with a linear plasmid DNA and products were analyzed of the reaction through gel electrophoresis (Figure 3). SegB cleaved DNA by introducing double-strand breaks in reactions requiring Mg2+, Mn2+, or Ca2+ cations. With Mg2+ and Ca2+ cation, SegB cleaved the DNA at 1 point, creating 2.2 kb and 1.6 kb fragments. In the presence of Mn2+, multiple cleavage sites were present.

complete
CACAO 13238

involved_in

GO:0090305

nucleic acid phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0004519

P

Seeded From UniProt

complete

involved_in

GO:0090305

nucleic acid phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0004518

P

Seeded From UniProt

complete

enables

GO:0004519

endonuclease activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0255

F

Seeded From UniProt

complete

enables

GO:0016787

hydrolase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0378

F

Seeded From UniProt

complete

enables

GO:0004518

nuclease activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0540

F

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Brok-Volchanskaya, VS et al. (2008) Phage T4 SegB protein is a homing endonuclease required for the preferred inheritance of T4 tRNA gene region occurring in co-infection with a related phage. Nucleic Acids Res. 36 2094-105 PubMed GONUTS page