User:Devangam

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	BIFLO:Q8G545	2012-04-15 11:35:44 CDT	GO:0004360 glutamine-fructose-6-phosphate transaminase (isomerizing) activity (F)	PMID:17943273	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 is a high-performance liquid chromatography study that detects elution profiles of phosphorylated monosaccharides. When purified GlmS protein was used (the gene product of Bifidobacterium longum gene glmS), a peak for glucosamine-6-phosphate was observed with addition of fructose-6-phosphate and glutamine. This demonstrates glutamine fructose-6-phosphate amidotransferase activity.	challenge
acceptable	CAMJE:FABZ	2012-04-15 12:21:52 CDT	GO:0019171 3-hydroxyacyl-(acyl-carrier-protein) dehydratase activity (F)	PMID:19280690	ECO:0000314 direct assay evidence used in manual assertion	A spectrophotometric assay of fabZ activity is demonstrated by Figure 4. It shows the kinetics of fabZ dehydratase activity by showing the fabZ activity in response to varied levels of Crotonoyl-CoA and beta-hydroxybutyryl-CoA. Table 2 demonstrates that the Km value of beta-Hydroxybutyryl-CoA is greater than that of Crotonoyl-CoA, demonstrating that fabZ converts Beta-Hydroxybutyryl-CoA to Crotonoyl-CoA.	challenge
acceptable	SALTY:COBT	2012-04-15 12:46:28 CDT	GO:0008939 nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity (F)	PMID:8206834	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows a TLC plate's separation of the reactants and products of the S. typhimurium CobT protein reaction with 2 different cell extracts: one with chromosomally (normal) encoded levels of CobT, and another with overexpressed levels of CobT. The separation indicates that the CobT-overexpressing cells have much greater alpha-ribazole-5'-P and the normal cells have much greater DMB (5,6-dimethylbenzimidazole). The separation indicates that CobT activity catalyzes the reaction from DMB to alpha-ribazole-5'-P, thus demonstrating nicotinate-nucleotide-dimethylbenzimadazole phosphoribosyltransferase activity.	challenge
requireschanges	MYCTU:CLPX	2012-04-15 15:16:57 CDT	GO:0051302 regulation of cell division (P)	PMID:20625433	ECO:0000314 direct assay evidence used in manual assertion	Figure 2A is a light scatter assay that shows the amount of FtsZ polymerization. FtsZ polymerization is necessary for FtsZ ring formation and cell division. Figure 2A shows that for M. tuberculosis mutant without a function ClpX gene has a higher level of FtsZ polymerization than the wild type strain. This indicates that ClpX inhibits cell division.	challenge
requireschanges	BACSU:PLSY	2012-04-15 15:37:14 CDT	GO:0016747 transferase activity, transferring acyl groups other than amino-acyl groups (F)	PMID:19282621	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 is a TLC plate that demonstrates the separation of protein fractions of wild type cells and protein fractions of mutant cells without PlsY gene. There is no separation for PlsY mutant cells when palmitoyl phosphate (an acyl phosphate) is added. This demonstrates that PlsY has acyl-phosphate transferase activity.	challenge
requireschanges	BRUAO:Q9FDD0	2012-04-15 15:55:22 CDT	GO:0045333 cellular respiration (P)	PMID:11274104	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 demonstrates the survival rate of multiple strains of Brucella abortus. Strain BA582pSEK102 contains the cydB gene, and has a higher survival rate in response to respiratory inhibitors, whereas strain BA582 which lacks cydB gene, has a much lower survival rate in response to respiratory inhibitors. This indicates that cydB gene is involved in facilitating cellular respiration.	challenge
requireschanges	PROMI:C2LKP1	2012-04-15 16:04:37 CDT	GO:0042710 biofilm formation (Figure 2 is a graph showing a viable cell count biofilm assay comparing the biofilm formation and growth of Wild type P. mirabilis cells, mutant cells without pstA gene, and mutant cells without pstS gene. The pstA and pstS mutants have much less biofilm formation than the wild type, indicating that the Pst family genes are involved in biofilm formation. )	PMID:19372157	IDA: Inferred from Direct Assay		challenge

acceptable:2
unacceptable:0
requires_changes:5
flagged:0

Annotations challenged by Devangam

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Devangam

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	GEOSE:NEPU	2012-03-29 11:23:40 CDT	GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds (F)	PMID:209598	ECO:0000314 direct assay evidence used in manual assertion	Figures 1 & 2 demonstrates results from paper chromatograms that shows amount of sugars present over time when treated with neopullulanase. The increasing amounts of maltose and glucose that the enzyme degrades maltotriose, maltotriitol, isopanose, maltotetraose, 6^3-O-alpha-glucosyl-maltotriose, and 6^2-O-alpha-maltosyl-maltose.	challenge
requireschanges	GEOSE:NEPU	2012-03-29 11:36:32 CDT	GO:0005975 carbohydrate metabolic process (P)	PMID:123897	ECO:0000314 direct assay evidence used in manual assertion	Figure 1 shows results from a TLC plate that demonstrates that neopullulanase degrades amylose and small amounts of amylopectin into smaller subunits, mainly maltose and glucose. The TLC plate shows relative degradation of amylose and amylopectin, and it demonstrates that neopullulanase degrades amylose into glucose and maltose much more than it degrades amylopectin into glucose and maltose. Figure 2 is gel permeation chromatography that demonstrates that neopullulanase degrades amylose more than amylopectin over time. The degradation of starch by neopullulanase is evidence that it is involved in carbohydrate metabolism.	challenge

acceptable:0
unacceptable:0
requires_changes:2
flagged:0

Annotations challenged by Devangam

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Devangam