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PMID:19280690
| Citation |
Kirkpatrick, AS, Yokoyama, T, Choi, KJ and Yeo, HJ (2009) Campylobacter jejuni fatty acid synthase II: structural and functional analysis of beta-hydroxyacyl-ACP dehydratase (FabZ). Biochem. Biophys. Res. Commun. 380:407-12 |
|---|---|
| Abstract |
Fatty acid biosynthesis is crucial for all living cells. In contrast to higher organisms, bacteria use a type II fatty acid synthase (FAS II) composed of a series of individual proteins, making FAS II enzymes excellent targets for antibiotics discovery. The beta-hydroxyacyl-ACP dehydratase (FabZ) catalyzes an essential step in the FAS II pathway. Here, we report the structure of Campylobacter jejuni FabZ (CjFabZ), showing a hexamer both in crystals and solution, with each protomer adopting the characteristic hot dog fold. Together with biochemical analysis of CjFabZ, we define the first functional FAS II enzyme from this pathogen, and provide a framework for investigation on roles of FAS II in C. jejuni virulence. |
| Links |
PubMed PMC2914181 Online version:10.1016/j.bbrc.2009.01.115 |
| Keywords |
Amino Acid Sequence; Campylobacter jejuni/enzymology; Catalysis; Crystallography, X-Ray; Fatty Acid Synthetase Complex, Type II/chemistry; Fatty Acid Synthetase Complex, Type II/metabolism; Fatty Acids/biosynthesis; Hydro-Lyases/chemistry; Hydro-Lyases/metabolism; Molecular Sequence Data; Protein Conformation |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019171: 3-hydroxyacyl-(acyl-carrier-protein) dehydratase activity |
ECO:0000314: |
F |
A spectrophotometric assay of fabZ activity is demonstrated by Figure 4. It shows the kinetics of fabZ dehydratase activity by showing the fabZ activity in response to varied levels of Crotonoyl-CoA and beta-hydroxybutyryl-CoA. Table 2 demonstrates that the Km value of beta-Hydroxybutyryl-CoA is greater than that of Crotonoyl-CoA, demonstrating that fabZ converts Beta-Hydroxybutyryl-CoA to Crotonoyl-CoA. |
complete | ||||
|
enables |
GO:0019171: 3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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