GEOSE:NEPU

Species (Taxon ID)	Geobacillus stearothermophilus (Bacillus stearothermophilus). (1422)
Gene Name(s)	nplT
Protein Name(s)	Neopullulanase
External Links
UniProt	P38940
EMBL	M28138
PIR	A37008
PDB	1J0H 1J0I 1J0J 1J0K
PDBsum	1J0H 1J0I 1J0J 1J0K
ProteinModelPortal	P38940
SMR	P38940
CAZy	CBM34 GH13
EvolutionaryTrace	P38940
GO	GO:0046872 GO:0031216 GO:0005975
Gene3D	2.60.40.10 2.60.40.1180 3.20.20.80
InterPro	IPR015902 IPR013780 IPR006047 IPR004185 IPR006589 IPR013781 IPR017853 IPR013783
PANTHER	PTHR10357
Pfam	PF00128 PF02903
SMART	SM00642
SUPFAM	SSF51445

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	PMID:PMC209598	ECO:0000314			F		Figures 1 & 2 demonstrates results from paper chromatograms that shows amount of sugars present over time when treated with neopullulanase. The increasing amounts of maltose and glucose that the enzyme degrades maltotriose, maltotriitol, isopanose, maltotetraose, 6^3-O-alpha-glucosyl-maltotriose, and 6^2-O-alpha-maltosyl-maltose.	complete CACAO 4049
	GO:0005975	carbohydrate metabolic process	PMID:PMC123897	ECO:0000314			P		Figure 1 shows results from a TLC plate that demonstrates that neopullulanase degrades amylose and small amounts of amylopectin into smaller subunits, mainly maltose and glucose. The TLC plate shows relative degradation of amylose and amylopectin, and it demonstrates that neopullulanase degrades amylose into glucose and maltose much more than it degrades amylopectin into glucose and maltose. Figure 2 is gel permeation chromatography that demonstrates that neopullulanase degrades amylose more than amylopectin over time. The degradation of starch by neopullulanase is evidence that it is involved in carbohydrate metabolism.	complete CACAO 4056
enables	GO:0003824	catalytic activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006047	F		Seeded From UniProt	complete
enables	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004185	F		Seeded From UniProt	complete
involved_in	GO:0005975	carbohydrate metabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004185 InterPro:IPR006047	P		Seeded From UniProt	complete
enables	GO:0031216	neopullulanase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.2.1.135	F		Seeded From UniProt	complete
enables	GO:0016798	hydrolase activity, acting on glycosyl bonds	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0326	F		Seeded From UniProt	complete
enables	GO:0046872	metal ion binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0479	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
involved_in	GO:0008152	metabolic process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0326	P		Seeded From UniProt	complete
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References

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