User:Danielle.ocallaghan

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	HUMAN:IGF1R	2012-10-18 09:45:35 CDT	GO:0001006 RNA polymerase III type 3 promoter sequence-specific DNA binding (F)	PMID:23071652	ECO:0000314 direct assay evidence used in manual assertion	Figure 2. The standard curves of IGF signaling antibody array. This graph shows the binding sites of the DNA	challenge
requireschanges	LAMBD:TFA	2012-10-18 12:09:03 CDT	GO:0043815 phosphoribosylglycinamide formyltransferase 2 activity (F)	PMID:333436	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1: The finding that gen.63 am mutations eliminate both TFA and RNA ligase activities suggests that both may reside in the protein gp63. This suggestion was proven correct in two ways. First, in amM69-infected supF host cells, both TFA and RNA ligase activities were shown to be markedly more heat labile than the corresponding activities in standard-type T4D-infected supF cells (Fig. 1). This result indicates that gp63 is responsible for both activities.	challenge
requireschanges	LAMBD:TFA	2012-10-18 12:16:19 CDT	GO:0043815 phosphoribosylglycinamide formyltransferase 2 activity (F)	PMID:333436	ECO:0000314 direct assay evidence used in manual assertion	Table 4: Assay B for TFA and the standard assay for RNA cyclization were carried out with 0.3 and 0.003 jig ofRNA ligase, respectively. Both tail fibers and RNA ligase were filtered through Sephadex G-50 before use. Extents of TFA and RNA cyclization were measured after 0, 15, 30, 60, 120, and 180 min. The rates observed in the complete reactions (k value for TFA = 0.008 min-') were set equal to 100.	challenge
updatedbyinstructor	BPT4:RLIG	2012-10-31 20:54:56 CDT	GO:0003972 RNA ligase (ATP) activity (F)	PMID:333436	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1	challenge
acceptable	BPT4:RLIG	2012-11-15 08:00:25 CST	GO:0098004 virus tail fiber assembly (P)	PMID:333436	ECO:0000315 mutant phenotype evidence used in manual assertion	RNA ligase promotes tail fiber attachment. The product of gene 63 is known to promote attachment of tail fibers to the phage baseplate in the terminal step of T4 phage assembly. See figure 1	challenge
updatedbyinstructor	BPT4:NEEDL	2012-11-15 08:12:37 CST	GO:0098025 virus tail, baseplate (C)	PMID:3157805	ECO:0000314 direct assay evidence used in manual assertion	The tail-lysozyme was unambiguously identified as gp5 when the position of the lysozyme was compared with that of gp5 of tube-baseplates from 5ts1/23amH11/eL1ainfected Escherichia coli cells by two-dimensional gel electrophoresis. see figure 4	challenge

acceptable:1
unacceptable:1
requires_changes:2
flagged:0

Annotations challenged by Danielle.ocallaghan

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Danielle.ocallaghan