PMID:333436

Citation	Snopek, TJ, Wood, WB, Conley, MP, Chen, P and Cozzarelli, NR (1977) Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate. Proc. Natl. Acad. Sci. U.S.A. 74:3355-9
Abstract	RNA ligase and tail fiber attachment activities, normally induced following bacteriophage T4 infection of Escherichia coli, are not induced when gene 63 amber mutants of T4 infect nonpermissive host cells. Both activities are induced when these mutants infect permissive hosts, or when revertants of these mutants infect nonpermissive hosts. When one of these mutants infects a host that carries supF, both activities are more than normally heat labile. RNA ligase, purified to homogeneity, promotes the tail fiber attachment reaction in vitro with a specific activity similar to that of the most highly purified preparations of gene 63 product isolated on the basis of tail fiber attachment activity. We conclude that T4 RNA ligase is gene 63 product. The RNA ligase and tail fiber attachment reactions differ in requirements and in response to some inhibitors, suggesting that the two activities of the gene 63 product may be mechanistically unrelated.
Links	PubMed PMC431559
Keywords	Coliphages/enzymology; DNA-Directed DNA Polymerase/metabolism; Escherichia coli/enzymology; Genes, Viral; Kinetics; Mutation; Polynucleotide Ligases/metabolism; Protein Denaturation; RNA Ligase (ATP)/genetics; RNA Ligase (ATP)/isolation & purification; RNA Ligase (ATP)/metabolism; Viral Proteins/genetics; Viral Proteins/metabolism; Virus Replication
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
LAMBD:TFA		GO:0043815: phosphoribosylglycinamide formyltransferase 2 activity	ECO:0000314:		F		Table 4: Assay B for TFA and the standard assay for RNA cyclization were carried out with 0.3 and 0.003 jig ofRNA ligase, respectively. Both tail fibers and RNA ligase were filtered through Sephadex G-50 before use. Extents of TFA and RNA cyclization were measured after 0, 15, 30, 60, 120, and 180 min. The rates observed in the complete reactions (k value for TFA = 0.008 min-') were set equal to 100.	complete CACAO 5375
LAMBD:TFA		GO:0043815: phosphoribosylglycinamide formyltransferase 2 activity	ECO:0000315:		F		Figure 1: The finding that gen.63 am mutations eliminate both TFA and RNA ligase activities suggests that both may reside in the protein gp63. This suggestion was proven correct in two ways. First, in amM69-infected supF host cells, both TFA and RNA ligase activities were shown to be markedly more heat labile than the corresponding activities in standard-type T4D-infected supF cells (Fig. 1). This result indicates that gp63 is responsible for both activities.	complete CACAO 5374
BPT4:RLIG		GO:0098004: virus tail fiber assembly	ECO:0000315:		P		RNA ligase promotes tail fiber attachment. The product of gene 63 is known to promote attachment of tail fibers to the phage baseplate in the terminal step of T4 phage assembly. See figure 1	complete CACAO 5845
BPT4:RLIG		GO:0003972: RNA ligase (ATP) activity	ECO:0000315:		F		Fig 1	complete CACAO 5571
BPT4:RLIG	enables	GO:0003972: RNA ligase (ATP) activity	ECO:0000315: mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
BPT4:RLIG	involved_in	GO:0098004: virus tail fiber assembly	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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