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PMID:3157805

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Citation

Nakagawa, H, Arisaka, F and Ishii, S (1985) Isolation and characterization of the bacteriophage T4 tail-associated lysozyme. J. Virol. 54:460-6

Abstract

Direct evidence has been obtained that the tail-associated lysozyme of bacteriophage T4 (tail-lysozyme) is gp5, which is a protein component of the hub of the baseplate. Tails were treated with 3 M guanidine hydrochloride containing 1% Triton X-100, and the tail-lysozyme was separated from other tail components by preparative isoelectric focusing electrophoresis as a peak with a pI of 8.4. The molecular weight as determined from sodium dodecyl sulfate electrophoresis was 42,000. The tail-lysozyme was unambiguously identified as gp5 when the position of the lysozyme was compared with that of gp5 of tube-baseplates from 5ts1/23amH11/eL1ainfected Escherichia coli cells by two-dimensional gel electrophoresis. The tail-lysozyme has N-acetylmuramidase activity and the same substrate specificity as gene e lysozyme; the optimum pH is around 5.8, about 1 pH unit lower than for the e lysozyme. We assume that the tail-lysozyme plays an essential role in locally digesting the peptidoglycan layer to let the tube penetrate into the periplasmic space. The tail-lysozyme is presumably also responsible for "lysis from without."

Links

PubMed PMC254817

Keywords

Hydrogen-Ion Concentration; Molecular Weight; Muramidase/isolation & purification; Muramidase/physiology; Mutation; Substrate Specificity; T-Phages/enzymology; Viral Proteins/analysis; Viral Tail Proteins

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPT4:NEEDL

enables

GO:0003796: lysozyme activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BPT4:NEEDL

involved_in

GO:0044409: entry into host

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BPT4:NEEDL

GO:0098025: virus tail, baseplate

ECO:0000314:

C

The tail-lysozyme was unambiguously identified as gp5 when the position of the lysozyme was compared with that of gp5 of tube-baseplates from 5ts1/23amH11/eL1ainfected Escherichia coli cells by two-dimensional gel electrophoresis. see figure 4

complete
CACAO 5848

BPT4:NEEDL

GO:0033922: peptidoglycan beta-N-acetylmuramidase activity

ECO:0000314:

F

Table 1 shows the results of an assay in which purified gp5 digested peptidoglycan. The loss of muramic acid and the appearance of muramicitol is consistent with an acetylmuramidase.

complete
CACAO 5594

BPT4:BP5

involved_in

GO:0085027: entry into host via enzymatic degradation of host anatomical structure

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

BPT4:BP5

part_of

GO:0098025: virus tail, baseplate

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPT4:BP5

enables

GO:0033922: peptidoglycan beta-N-acetylmuramidase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

BPT4:BP5

enables

GO:0003796: lysozyme activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

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