User:Cedwards2010

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BOVIN:GALT1	2012-02-07 19:49:51 CST	GO:0004653 polypeptide N-acetylgalactosaminyltransferase activity (F)	PMID:9359852	ECO:0000314 direct assay evidence used in manual assertion	Table 2 is a list of Km values determined from an kinetics experiment, the purified wild-type is the first entry in table, and all mutant proteins are normalized to wild-type activity. The glycoproteins formed were separated later with anion-exchanged chromatography.	challenge
acceptable	NEUCR:AAP	2012-02-12 14:03:37 CST	GO:0009386 translational attenuation (P)	PMID:9819438	ECO:0000314 direct assay evidence used in manual assertion	Figure 5A. Arginine Attenuator Pepetide gene is placed upstream of luciferase in a mRNA construct created for experiment. Shows decreased luciferase production in 500 uM vs 10 uM assay.	challenge
acceptable	HUMAN:EIF1	2012-02-21 20:30:09 CST	GO:0006446 regulation of translational initiation (P)	PMID:22156057	ECO:0000314 direct assay evidence used in manual assertion	Fig 2-C,D Show that that over expression of EIF1 protein leads to decreased expression of firefly luciferase constructs downstream of a poor-context AUG start codons and other non-AUG start codons.	challenge
acceptable	HUMAN:IF5	2012-02-21 20:30:15 CST	GO:0006446 regulation of translational initiation (P)	PMID:22156057	ECO:0000314 direct assay evidence used in manual assertion	Fig 2-C,D Show that that over expression of EIF5 protein leads to increased expression of firefly luciferase constructs downstream of a poor-context AUG start codons and other non-AUG start codons.	challenge
acceptable	STRPA:GTF1	2012-03-06 20:22:28 CST	GO:0016262 protein N-acetylglucosaminyltransferase activity (F)	PMID:20971868	ECO:0000314 direct assay evidence used in manual assertion	Figured 2C. GTF1 was tandem purified with GTF2 and assayed with proteins using radioactive labeled UDP-GlcNAc	challenge
updatedbyinstructor	STRPA:GTF2	2012-03-06 20:32:09 CST	GO:0017122 protein N-acetylglucosaminyltransferase complex (C)	PMID:20971868	ECO:0000314 direct assay evidence used in manual assertion	Figure 2-C GTF2 is required for maximal activity of the GlcNAc transferase activity. Figure 1 shows the results of SDS-PAGE electrophoresis after TAP purification of the protein complex, proteins co-purify together under native conditions.	challenge
acceptable	STRPA:GTF1	2012-03-06 20:34:51 CST	GO:0017122 protein N-acetylglucosaminyltransferase complex (C)	PMID:20971868	ECO:0000314 direct assay evidence used in manual assertion	Figure 2-C GTF1 achieves maximal activity when complexed with GTF2. Figure 1 shows the results of SDS-PAGE electrophoresis after TAP purification of the protein complex, proteins co-purify together under native conditions.	challenge
acceptable	ECOCB:D3QY14	2012-03-27 20:05:36 CDT	GO:0048531 beta-1,3-galactosyltransferase activity (F)	PMID:19560364	ECO:0000314 direct assay evidence used in manual assertion	Table 1 shows the radioactivity due to incorporated [6-3H]Gal to the acceptor molecule.	challenge
updatedbyinstructor	SHIBO:WFED	2012-03-27 20:23:02 CDT	GO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity (F)	PMID:21057010	ECO:0000314 direct assay evidence used in manual assertion	Table 2 shows the relative activities of the purified enzymes on different acceptor substrates. The enzymatic assay uses tritium labeled radioactive UDP-galactose. Linkage confirmed with Beta-1,4 specific galactosidases (jack bean and bovine testicular).	challenge
updatedbyinstructor	MORCA:Q5DIA0	2012-04-01 13:03:10 CDT	GO:0046527 glucosyltransferase activity (F)	PMID:15838019	ECO:0000316 genetic interaction evidence used in manual assertion	Figure 4. H. ducreyi 35000glu-, a mutant deficient in a specific beta (1,4) glucosyltransferase(lgtF) was rescued by complementation with M. catarrhalis 7169 lgt3. Assay utilizes a screen with MAb 1B2-1B7, which recognizes full length LOS.	challenge
updatedbyinstructor	MORCA:Q5DIA2	2012-04-01 13:17:06 CDT	GO:0046527 glucosyltransferase activity (F)	PMID:15838019	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 2 and 3. Fig 2, mutant missing lgt1 loses activity with MAb-4G5 and MAb-3F7. Figure 3 structural analysis and comparison from MALDI-TOF mass-spec on products of mutants and revertants suggests function as a 1,2-alpha-glucosyltransferase.	challenge
acceptable	MORCA:B0EWZ9	2012-04-01 13:26:16 CDT	GO:0008378 galactosyltransferase activity (F)	PMID:15838019	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 3. MALDI-TOF mass spec comparison of products from mutants and revertants shows truncation at galactose addition site, suspected function as a 1,4-beta-galactosyltransferase.	challenge
acceptable	YEREN:Q56914	2012-04-01 14:03:15 CDT	GO:0047270 lipopolysaccharide glucosyltransferase II activity (F)	PMID:20595390	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 4, S2, and S3. Mutant lacks terminal glucose residue on LOS chain.	challenge
acceptable	YEREN:Q56915	2012-04-01 14:09:30 CDT	GO:0008919 lipopolysaccharide glucosyltransferase I activity (F)	PMID:20595390	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 4, S2, S3. MALDI MS of mutant products shows lack of glucose side chain from LOS chain.	challenge
acceptable	YEREN:Q56916	2012-04-01 14:17:24 CDT	GO:0008376 acetylgalactosaminyltransferase activity (F)	PMID:20595390	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 4, S2, and S3. Mutant terminates before GalNAc residue on LOS chain.	challenge
acceptable	YEREN:Q56917	2012-04-10 19:42:12 CDT	GO:0035250 UDP-galactosyltransferase activity (F)	PMID:20595390	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 1, Table 4. Chemical analysis of YeO3-c-wbcN1 mutant revealed truncated OC structure containing only Sugp and GalNAc residues. WT Chemotype of OC was restored by complementation with WT wbcN.	challenge
updatedbyinstructor	STRPA:GTF2	2012-04-10 20:03:47 CDT	GO:0016262 protein N-acetylglucosaminyltransferase activity (F)	PMID:20971868	ECO:0000314 direct assay evidence used in manual assertion	Figured 2C. GTF1 was tandem purified with GTF2 and assayed with proteins using radioactive labeled UDP-GlcNAc	challenge

acceptable:12
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Cedwards2010

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Taustin, Team Big Mama 50000	MYCSD:F5Z3Z0	GO:0016740 - transferase activity (F)	PMID:22119138	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Cedwards2010