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Category:Team Rainbow Buddy Huddle

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableMOUSE:TERTSolmathew, Team Rainbow Buddy Huddle2012-04-15 11:52:05 CDTGO:0005697 telomerase holoenzyme complex (C)PMID:22496369ECO:0000314 direct assay evidence used in manual assertion

In figure 8, it shows that p73 promoters represses the TERT complex.

challenge
updatedbyinstructorSTRPA:GTF2Cedwards2010, Team Rainbow Buddy Huddle2012-04-10 20:03:47 CDTGO:0016262 protein N-acetylglucosaminyltransferase activity (F)PMID:20971868ECO:0000314 direct assay evidence used in manual assertion

Figured 2C. GTF1 was tandem purified with GTF2 and assayed with proteins using radioactive labeled UDP-GlcNAc

challenge
acceptableYEREN:Q56917Cedwards2010, Team Rainbow Buddy Huddle2012-04-10 19:42:12 CDTGO:0035250 UDP-galactosyltransferase activity (F)PMID:20595390ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1, Table 4. Chemical analysis of YeO3-c-wbcN1 mutant revealed truncated OC structure containing only Sugp and GalNAc residues. WT Chemotype of OC was restored by complementation with WT wbcN.

challenge
acceptableYEREN:Q56916Cedwards2010, Team Rainbow Buddy Huddle2012-04-01 14:17:24 CDTGO:0008376 acetylgalactosaminyltransferase activity (F)PMID:20595390ECO:0000315 mutant phenotype evidence used in manual assertion

Table 4, S2, and S3. Mutant terminates before GalNAc residue on LOS chain.

challenge
acceptableYEREN:Q56915Cedwards2010, Team Rainbow Buddy Huddle2012-04-01 14:09:30 CDTGO:0008919 lipopolysaccharide glucosyltransferase I activity (F)PMID:20595390ECO:0000315 mutant phenotype evidence used in manual assertion

Table 4, S2, S3. MALDI MS of mutant products shows lack of glucose side chain from LOS chain.

challenge
acceptableYEREN:Q56914Cedwards2010, Team Rainbow Buddy Huddle2012-04-01 14:03:15 CDTGO:0047270 lipopolysaccharide glucosyltransferase II activity (F)PMID:20595390ECO:0000315 mutant phenotype evidence used in manual assertion

Table 4, S2, and S3. Mutant lacks terminal glucose residue on LOS chain.

challenge
acceptableMORCA:B0EWZ9Cedwards2010, Team Rainbow Buddy Huddle2012-04-01 13:26:16 CDTGO:0008378 galactosyltransferase activity (F)PMID:15838019ECO:0000315 mutant phenotype evidence used in manual assertion

Figures 3. MALDI-TOF mass spec comparison of products from mutants and revertants shows truncation at galactose addition site, suspected function as a 1,4-beta-galactosyltransferase.

challenge
updatedbyinstructorMORCA:Q5DIA2Cedwards2010, Team Rainbow Buddy Huddle2012-04-01 13:17:06 CDTGO:0046527 glucosyltransferase activity (F)PMID:15838019ECO:0000315 mutant phenotype evidence used in manual assertion

Figures 2 and 3. Fig 2, mutant missing lgt1 loses activity with MAb-4G5 and MAb-3F7. Figure 3 structural analysis and comparison from MALDI-TOF mass-spec on products of mutants and revertants suggests function as a 1,2-alpha-glucosyltransferase.

challenge
updatedbyinstructorMORCA:Q5DIA0Cedwards2010, Team Rainbow Buddy Huddle2012-04-01 13:03:10 CDTGO:0046527 glucosyltransferase activity (F)PMID:15838019ECO:0000316 genetic interaction evidence used in manual assertion

Figure 4. H. ducreyi 35000glu-, a mutant deficient in a specific beta (1,4) glucosyltransferase(lgtF) was rescued by complementation with M. catarrhalis 7169 lgt3. Assay utilizes a screen with MAb 1B2-1B7, which recognizes full length LOS.

challenge
updatedbyinstructorSHIBO:WFEDCedwards2010, Team Rainbow Buddy Huddle2012-03-27 20:23:02 CDTGO:0003831 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity (F)PMID:21057010ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows the relative activities of the purified enzymes on different acceptor substrates. The enzymatic assay uses tritium labeled radioactive UDP-galactose. Linkage confirmed with Beta-1,4 specific galactosidases (jack bean and bovine testicular).

challenge
acceptableECOCB:D3QY14Cedwards2010, Team Rainbow Buddy Huddle2012-03-27 20:05:36 CDTGO:0048531 beta-1,3-galactosyltransferase activity (F)PMID:19560364ECO:0000314 direct assay evidence used in manual assertion

Table 1 shows the radioactivity due to incorporated [6-3H]Gal to the acceptor molecule.

challenge
acceptableSTRPA:GTF1Cedwards2010, Team Rainbow Buddy Huddle2012-03-06 20:34:51 CSTGO:0017122 protein N-acetylglucosaminyltransferase complex (C)PMID:20971868ECO:0000314 direct assay evidence used in manual assertion

Figure 2-C GTF1 achieves maximal activity when complexed with GTF2. Figure 1 shows the results of SDS-PAGE electrophoresis after TAP purification of the protein complex, proteins co-purify together under native conditions.

challenge
updatedbyinstructorSTRPA:GTF2Cedwards2010, Team Rainbow Buddy Huddle2012-03-06 20:32:09 CSTGO:0017122 protein N-acetylglucosaminyltransferase complex (C)PMID:20971868ECO:0000314 direct assay evidence used in manual assertion

Figure 2-C GTF2 is required for maximal activity of the GlcNAc transferase activity. Figure 1 shows the results of SDS-PAGE electrophoresis after TAP purification of the protein complex, proteins co-purify together under native conditions.

challenge
acceptableSTRPA:GTF1Cedwards2010, Team Rainbow Buddy Huddle2012-03-06 20:22:28 CSTGO:0016262 protein N-acetylglucosaminyltransferase activity (F)PMID:20971868ECO:0000314 direct assay evidence used in manual assertion

Figured 2C. GTF1 was tandem purified with GTF2 and assayed with proteins using radioactive labeled UDP-GlcNAc

challenge
acceptableHUMAN:IF5Cedwards2010, Team Rainbow Buddy Huddle2012-02-21 20:30:15 CSTGO:0006446 regulation of translational initiation (P)PMID:22156057ECO:0000314 direct assay evidence used in manual assertion

Fig 2-C,D Show that that over expression of EIF5 protein leads to increased expression of firefly luciferase constructs downstream of a poor-context AUG start codons and other non-AUG start codons.

challenge
acceptableHUMAN:EIF1Cedwards2010, Team Rainbow Buddy Huddle2012-02-21 20:30:09 CSTGO:0006446 regulation of translational initiation (P)PMID:22156057ECO:0000314 direct assay evidence used in manual assertion

Fig 2-C,D Show that that over expression of EIF1 protein leads to decreased expression of firefly luciferase constructs downstream of a poor-context AUG start codons and other non-AUG start codons.

challenge
requireschangesCAEEL:KNL1Solmathew, Team Rainbow Buddy Huddle2012-02-21 19:45:20 CSTGO:0051382 kinetochore assembly (P)PMID:22331849ECO:0000315 mutant phenotype evidence used in manual assertion

Knl-1 is vital for signaling and attachment of microtubules to the KMN network as seen in video 1.

challenge
updatedbyinstructorBACSU:RIDASolmathew, Team Rainbow Buddy Huddle2012-02-12 14:03:46 CSTGO:0046360 2-oxobutyrate biosynthetic process (P)PMID:22094463ECO:0000314 direct assay evidence used in manual assertion

fig 2A

challenge
acceptableNEUCR:AAPCedwards2010, Team Rainbow Buddy Huddle2012-02-12 14:03:37 CSTGO:0009386 translational attenuation (P)PMID:9819438ECO:0000314 direct assay evidence used in manual assertion

Figure 5A. Arginine Attenuator Pepetide gene is placed upstream of luciferase in a mRNA construct created for experiment. Shows decreased luciferase production in 500 uM vs 10 uM assay.

challenge
acceptableBOVIN:GALT1Cedwards2010, Team Rainbow Buddy Huddle2012-02-07 19:49:51 CSTGO:0004653 polypeptide N-acetylgalactosaminyltransferase activity (F)PMID:9359852ECO:0000314 direct assay evidence used in manual assertion

Table 2 is a list of Km values determined from an kinetics experiment, the purified wild-type is the first entry in table, and all mutant proteins are normalized to wild-type activity. The glycoproteins formed were separated later with anion-exchanged chromatography.

challenge
requireschangesBOVIN:A7E3R3Solmathew, Team Rainbow Buddy Huddle2012-02-07 19:48:45 CSTGO:0044454 nuclear chromosome part (C)PMID:22266654ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 demonstrates how the TRF1 function on the chromosome is cell cycle regulated.

challenge

Pages in category "Team Rainbow Buddy Huddle"

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