GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:19560364

From GONUTS
Jump to: navigation, search
Citation

Liu, XW, Xia, C, Li, L, Guan, WY, Pettit, N, Zhang, HC, Chen, M and Wang, PG (2009) Characterization and synthetic application of a novel beta1,3-galactosyltransferase from Escherichia coli O55:H7. Bioorg. Med. Chem. 17:4910-5

Abstract

A beta1,3-galactosyltransferase (WbgO) was identified in Escherichia coli O55:H7. Its function was confirmed by radioactive activity assay and structure analysis of the disaccharide synthesized with the recombinant enzyme. WbgO requires a divalent metal ion, either Mn(2+) or Mg(2+), for its activity and is active between pH 6.0-8.0 with a pH optimum of 7.0. N-acetylglucosamine (GlcNAc) and oligosaccharides with GlcNAc at the non-reducing end were shown to be its preferred substrates and it can be used for the synthesis of type 1 glycan chains from these substrates. Together with a recombinant bacterial GlcNAc-transferase, benzyl beta-lacto-N-tetraoside was synthesized with the purified WbgO to demonstrate the synthetic utility of WbgO.

Links

PubMed Online version:10.1016/j.bmc.2009.06.005

Keywords

Acetylglucosamine/metabolism; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Galactosyltransferases/analysis; Galactosyltransferases/genetics; Galactosyltransferases/isolation & purification; Galactosyltransferases/metabolism; Magnesium/metabolism; Manganese/metabolism; Molecular Sequence Data; N-Acetylglucosaminyltransferases/genetics; N-Acetylglucosaminyltransferases/metabolism; Oligosaccharides/chemical synthesis; Oligosaccharides/chemistry; Oligosaccharides/metabolism; Recombinant Proteins/analysis; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Alignment; Substrate Specificity

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOCB:D3QY14

GO:0048531: beta-1,3-galactosyltransferase activity

ECO:0000314:

F

Table 1 shows the radioactivity due to incorporated [6-3H]Gal to the acceptor molecule.

complete
CACAO 4024

ECOCB:D3QY14

enables

GO:0048531: beta-1,3-galactosyltransferase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete


See also

References

See Help:References for how to manage references in GONUTS.