User:Cavana55

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	ECOLI:DMLA	2012-04-10 12:11:15 CDT	GO:0046553 D-malate dehydrogenase (decarboxylating) activity (F)	PMID:17088549	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 (C) yeaU (dmlA) showed increase expression levels during growth on D-malate compared to growth on L-lactate providing evidence for its involvement in D-malate catabolism.	challenge
acceptable	ARATH:METK1	2012-04-10 13:10:24 CDT	GO:0004478 methionine adenosyltransferase activity (F)	PMID:2535470	ECO:0000314 direct assay evidence used in manual assertion	Table 1 shows the results of AdoMet Sythetase Assays where the stem extract contained 10 times more AdoMet Sythetase activity than in leaf extract. Along with figure 2(a) which showed increased expression of sam-1 gene in stem extracts compared to leaf extracts.	challenge
acceptable	SOLTU:G9JKC3	2012-04-14 14:44:36 CDT	GO:0035251 UDP-glucosyltransferase activity (F)	PMID:9076990	ECO:0000314 direct assay evidence used in manual assertion	Figure 8 shows an increase in solanidine UDP-glucosyltransferase activity in response to wounding of potato tuber tissue.	challenge
requireschanges	ECOLI:LPLA	2012-04-15 17:04:44 CDT	GO:0016979 lipoate-protein ligase activity (F)	PMID:8206909	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows a defect in the accumulation of 36s-lipoyl proteins when lacking the lplA gene demonstrating lipoate-protein ligase activity.	challenge
requireschanges	ECOLI:LIPA	2012-04-15 17:44:07 CDT	GO:0016992 lipoate synthase activity (F)	PMID:8444795	ECO:0000314 direct assay evidence used in manual assertion	Figure 6 shows expression of lipA in a lipA+ strain compared to a mutant strain that is missing the lipA gene.	challenge

acceptable:2
unacceptable:0
requires_changes:3
flagged:0

Annotations challenged by Cavana55

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Cavana55

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	ECOLI:PROA	2012-03-29 15:53:19 CDT	GO:0047443 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity (F)	PMID:11826967	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 (B) shows a strain (containing the gene proA) grown in the presence of IPTG showing the same expression level as a purified strain of 4-hydroxy-4-methyl-2-oxoglutarate aldolase from P. ochraceae.	challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Cavana55

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Cavana55