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ECOLI:LIPA
Contents
| Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
| Gene Name(s) | lipA (synonyms: lip) | |
| Protein Name(s) | Lipoyl synthase
Lip-syn LS Lipoate synthase Lipoic acid synthase Sulfur insertion protein LipA | |
| External Links | ||
| UniProt | P60716 | |
| EMBL | M82805 L07636 U82598 U00096 AP009048 | |
| PIR | B64797 | |
| RefSeq | NP_415161.1 YP_488919.1 | |
| ProteinModelPortal | P60716 | |
| SMR | P60716 | |
| DIP | DIP-48008N | |
| IntAct | P60716 | |
| MINT | MINT-1310776 | |
| STRING | 511145.b0628 | |
| PaxDb | P60716 | |
| PRIDE | P60716 | |
| EnsemblBacteria | AAC73729 BAA35271 | |
| GeneID | 12931644 945227 | |
| KEGG | ecj:Y75_p0618 eco:b0628 | |
| PATRIC | 32116437 | |
| EchoBASE | EB1283 | |
| EcoGene | EG11306 | |
| eggNOG | COG0320 | |
| HOGENOM | HOG000235997 | |
| InParanoid | P60716 | |
| KO | K03644 | |
| OMA | EEYVTPE | |
| OrthoDB | EOG6038ZS | |
| PhylomeDB | P60716 | |
| BioCyc | EcoCyc:EG11306-MONOMER ECOL316407:JW0623-MONOMER MetaCyc:EG11306-MONOMER | |
| UniPathway | UPA00538 | |
| PRO | PR:P60716 | |
| Proteomes | UP000000318 UP000000625 | |
| Genevestigator | P60716 | |
| GO | GO:0005737 GO:0051539 GO:0003826 GO:0016992 GO:0046872 GO:0009107 GO:0055114 GO:0009249 | |
| Gene3D | 3.20.20.70 | |
| HAMAP | MF_00206 | |
| InterPro | IPR013785 IPR006638 IPR003698 IPR007197 | |
| PANTHER | PTHR10949 | |
| Pfam | PF04055 | |
| PIRSF | PIRSF005963 | |
| SMART | SM00729 | |
| TIGRFAMs | TIGR00510 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0016992 |
lipoate synthase activity |
ECO:0000314 |
F |
Figure 6 shows expression of lipA in a lipA+ strain compared to a mutant strain that is missing the lipA gene. |
complete | |||||
|
NOT |
GO:0003826 |
alpha-ketoacid dehydrogenase activity |
ECO:0000315 |
F |
Seeded From UniProt The article referenced reports the effects of inactivating lipA and lipB on the alpha-ketoacid dehydrogenase activities of the PDH and KDGH complexes. ECOLI:LIPA should not be annotated with this activity. |
complete | ||||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
EcoGene:EG11306 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
NOT |
GO:0003826 |
alpha-ketoacid dehydrogenase activity |
ECO:0000315 |
F |
Table 2 in the article referenced reports the effects of inactivating lipA and lipB on the alpha-ketoacid dehydrogenase activities of the PDH and KDGH complexes. ECOLI:LIPA should not be annotated with this activity. Dsiegele (talk) 17:20, 11 November 2017 (CST) |
complete | ||||
|
enables |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016992 |
lipoate synthase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009249 |
protein lipoylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009107 |
lipoate biosynthetic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003824 |
catalytic activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009107 |
lipoate biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016992 |
lipoate synthase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051536 |
iron-sulfur cluster binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016992 |
lipoate synthase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000080080 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0009107 |
lipoate biosynthetic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000080080 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016992 |
lipoate synthase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000080080 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016783 |
sulfurtransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000080080 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0009249 |
protein lipoylation |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000080080 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000080080 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051539 |
4 iron, 4 sulfur cluster binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0051536 |
iron-sulfur cluster binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 Reed, KE & Cronan, JE Jr (1993) Lipoic acid metabolism in Escherichia coli: sequencing and functional characterization of the lipA and lipB genes. J. Bacteriol. 175 1325-36 PubMed GONUTS page
- ↑ Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 Cicchillo, RM et al. (2004) Lipoyl synthase requires two equivalents of S-adenosyl-L-methionine to synthesize one equivalent of lipoic acid. Biochemistry 43 6378-86 PubMed GONUTS page
- ↑ Ollagnier-De Choudens, S et al. (2000) Iron-sulfur center of biotin synthase and lipoate synthase. Biochemistry 39 4165-73 PubMed GONUTS page
- ↑ Cicchillo, RM et al. (2004) Escherichia coli lipoyl synthase binds two distinct [4Fe-4S] clusters per polypeptide. Biochemistry 43 11770-81 PubMed GONUTS page
- ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
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