PMID:2535470

Citation	Peleman, J, Boerjan, W, Engler, G, Seurinck, J, Botterman, J, Alliotte, T, Van Montagu, M and Inzé, D (1989) Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase. Plant Cell 1:81-93
Abstract	S-Adenosylmethionine serves as a methyl group donor in numerous transmethylation reactions and plays a role in the biosynthesis of polyamines and ethylene. We have cloned and sequenced an S-adenosylmethionine synthetase gene (sam-1) of Arabidopsis thaliana. The deduced polypeptide sequence of the enzyme has extensive homology with the corresponding enzymes of Escherichia coli and yeast. Genomic hybridization indicates the presence of two adenosylmethionine synthetase genes per haploid Arabidopsis genome. RNA gel blot analysis shows that adenosylmethionine synthetase mRNA levels are high in stems and roots, correlating well with the higher enzyme activity in stems, compared with leaves. Histochemical analysis of transgenic Arabidopsis plants transformed with a chimeric beta-glucuronidase gene, under the control of 748-base pair 5' sequences of the sam-1 gene, demonstrates that the gene is expressed primarily in vascular tissues. In addition, high expression was observed in sclerenchyma and in the root cortex. A hypothesis for the strong cellular preference in the expression of the sam-1 gene is presented.
Links	PubMed PMC159739 Online version:10.1105/tpc.1.1.81
Keywords	Amino Acid Sequence; Base Sequence; Blotting, Northern; Cloning, Molecular; DNA; Methionine Adenosyltransferase/genetics; Methionine Adenosyltransferase/metabolism; Molecular Sequence Data; Plant Cells; Plants/enzymology; Plants/genetics; Plants, Genetically Modified; Restriction Mapping; Sequence Alignment
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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