User:Ballchel

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	RHOPT:B3QHB6	2013-04-11 18:11:52 CDT	GO:0009279 cell outer membrane (C)	PMID:21873238	ECO:0000315 mutant phenotype evidence used in manual assertion	Encodes for essential proteins involved in intracellular hopanoid transport from the cytoplasmic to the outer membrane. Figure 4 shows the content of lipid extract from membrane fractions from R. palustris compared to the lipid extract from membranes of the mutant strain (Rpal_4254) where the gene was deleted. The WT contained 12.5 +/- 1 ug x mg total lipid extract (TLE); the mutant strain (Rpal_4254) no longer contained hopanoids in the outer membrane. Both WT and Rpal_4254 showed hopanoid content in the whole cell.	challenge
requireschanges	PSEAE:PQSD	2013-04-11 21:10:43 CDT	GO:0004315 3-oxoacyl-[acyl-carrier-protein] synthase activity (F)	PMID:18728009	ECO:0000314 direct assay evidence used in manual assertion	Figure 2 shows DHQ formation by PqsD in Vitro. Mutant strains showed PqsD was a part of DHQ synthesis in vivo and was further tested in vitro. Anthraniloyl-CoA was produced in situ by PqsA and reacted with malonyl-CoA (a product of PqsD) to form DHQ as supported by TLC and LC-MS. These tests confirm DHQ formation when compared against the DHQ standard. Figure 7 shows the proposed mechanism for DHQ synthesis with the products of the Pqs gene.	challenge
acceptable	ECOLX:E2D2R3	2013-04-14 12:57:09 CDT	GO:0050580 2,5-didehydrogluconate reductase activity (F)	PMID:19429550	ECO:0000316 genetic interaction evidence used in manual assertion	Figure 5: when expressed from plasmids, dkgA was found to decrease furfural tolerance. Figure 6: Deletions of dkgA caused an increase in furfural tolerance and a decrease in furfural reductase activity in vivo similar to the results shown in the EMFR9 strain of E. coli. Since deletion of dkgA alone did not lower the in vivo reductase activity or increase furfural tolerance, another gene, yqhD, is presumed to be the more important activity for growth inhibition by low concentrations of furfural. The lowest furfural reductase activity, however, was found after deletion of both genes as seen in Figure 3.	challenge

acceptable:1
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Ballchel

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Stanto81, Team Bringin back Fanny Packs	HUMAN:INSL3	GO:0010634 - positive regulation of epithelial cell migration (P)	PMID:23539510	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2 fixed by Stanto81

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