GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
CACAO Fall 2015
|Status||Page||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|HUMAN:BAG3||2015-10-14 09:53:51 CDT||GO:0005737 cytoplasm (C)||PMID:25149536||ECO:0000314 direct assay evidence used in manual assertion|
Immunohistochemical analysis of BAG3 expression in Figure 1 A, E and F.
|ECOLI:EX1||2015-11-02 10:46:00 CST||GO:0000175 3'-5'-exoribonuclease activity (F)||PMID:4927675||ECO:0000315 mutant phenotype evidence used in manual assertion|
As shown in Table 3, the wildtype strain of the gene sbcB shows exonuclease activity. When the anitserum, that inhibits exonuclease activity, was added the exonuclease activity decreased proving the exonuclease activity of the crude lysates. To verify the sbcB gene activity, the gene was mutated, sbcB15 and when mutated the exonuclease activity decreased to show that the sbcB gene is responsible for exonuclease activity.
|SHIFL:Q83M61||2015-11-17 12:08:56 CST||GO:0004519 endonuclease activity (F)||PMID:9133662||ECO:0000314 direct assay evidence used in manual assertion|
Both SbcC and SbcD polypeptides are required for the ATP-dependent double-strand exonuclease activity of SbcCD (Fig.3b) only the SbcD polypeptide is required for the ATP-independent single-strand endonuclease activity of SbcCD
|BUCAI:END1||2015-11-17 14:40:00 CST||GO:0004530 deoxyribonuclease I activity (F)||PMID:366||ECO:0000315 mutant phenotype evidence used in manual assertion|
Table 2 shows strain 577, which carries the end-14 mutation, is deficient in the major endonuclease
|ECOLX:TRAJ2||2015-11-17 16:32:35 CST||GO:0000746 conjugation (P)||PMID:24723101||ECO:0000314 direct assay evidence used in manual assertion|
The expression of traJ was activated at low concentration of bile salts and decreased with increasing bile salt concentration. The expression patterns of traJ were corresponded with the conjugative transfer frequencies between S. typhimurium and e. coli.
|ECOLI:DPO3A||2015-11-18 09:28:08 CST||GO:0003887 DNA-directed DNA polymerase activity (F)||PMID:16164549||ECO:0000315 mutant phenotype evidence used in manual assertion|
in Table 3 are the mutant DNA strains (dnaE486 and dnaE511) against the wildtype to show that the increasing mutations are caused by defects in the DNA polymerase III alpha subunit that are encoded by dnaE. The mutations of the dnaE prove to be responsible/involved in DNA polymerase activity.
|ECOLI:LGT||2015-11-18 11:45:25 CST||GO:0008961 phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity (F)||PMID:7896715||ECO:0000315 mutant phenotype evidence used in manual assertion|
These results (Fig. 1 and Table 1) show that E. coli umpA mutants (strains SK634, SK635, and SK636) were defective in diacylglyceryl modification of prolipoprotein because of defective diacylglyceryl transferase. Defective modification of prolipoprotein in umpA mutants was confirmed by in vitro analysis of prolipoprotein modification activity.
|SHIFL:MXIC||2015-11-18 14:49:15 CST||GO:0050709 negative regulation of protein secretion (P)||PMID:19017268||ECO:0000315 mutant phenotype evidence used in manual assertion|
Annotations challenged by Avf5209
|Status||Author,Group||Page||GO Term (Aspect)||Reference||Evidence||Links||Page history|
0 annotations fixed by Avf5209