User:Avf5209

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	HUMAN:BAG3	2015-10-14 09:53:51 CDT	GO:0005737 cytoplasm (C)	PMID:25149536	ECO:0000314 direct assay evidence used in manual assertion	Immunohistochemical analysis of BAG3 expression in Figure 1 A, E and F.	challenge
unacceptable	ECOLI:EX1	2015-11-02 10:46:00 CST	GO:0000175 3'-5'-exoribonuclease activity (F)	PMID:4927675	ECO:0000315 mutant phenotype evidence used in manual assertion	As shown in Table 3, the wildtype strain of the gene sbcB shows exonuclease activity. When the anitserum, that inhibits exonuclease activity, was added the exonuclease activity decreased proving the exonuclease activity of the crude lysates. To verify the sbcB gene activity, the gene was mutated, sbcB15 and when mutated the exonuclease activity decreased to show that the sbcB gene is responsible for exonuclease activity.	challenge
unacceptable	SHIFL:Q83M61	2015-11-17 12:08:56 CST	GO:0004519 endonuclease activity (F)	PMID:9133662	ECO:0000314 direct assay evidence used in manual assertion	Both SbcC and SbcD polypeptides are required for the ATP-dependent double-strand exonuclease activity of SbcCD (Fig.3b) only the SbcD polypeptide is required for the ATP-independent single-strand endonuclease activity of SbcCD	challenge
unacceptable	BUCAI:END1	2015-11-17 14:40:00 CST	GO:0004530 deoxyribonuclease I activity (F)	PMID:366	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 2 shows strain 577, which carries the end-14 mutation, is deficient in the major endonuclease	challenge
unacceptable	ECOLX:TRAJ2	2015-11-17 16:32:35 CST	GO:0000746 conjugation (P)	PMID:24723101	ECO:0000314 direct assay evidence used in manual assertion	The expression of traJ was activated at low concentration of bile salts and decreased with increasing bile salt concentration. The expression patterns of traJ were corresponded with the conjugative transfer frequencies between S. typhimurium and e. coli.	challenge
unacceptable	ECOLI:DPO3A	2015-11-18 09:28:08 CST	GO:0003887 DNA-directed DNA polymerase activity (F)	PMID:16164549	ECO:0000315 mutant phenotype evidence used in manual assertion	in Table 3 are the mutant DNA strains (dnaE486 and dnaE511) against the wildtype to show that the increasing mutations are caused by defects in the DNA polymerase III alpha subunit that are encoded by dnaE. The mutations of the dnaE prove to be responsible/involved in DNA polymerase activity.	challenge
acceptable	ECOLI:LGT	2015-11-18 11:45:25 CST	GO:0008961 phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity (F)	PMID:7896715	ECO:0000315 mutant phenotype evidence used in manual assertion	These results (Fig. 1 and Table 1) show that E. coli umpA mutants (strains SK634, SK635, and SK636) were defective in diacylglyceryl modification of prolipoprotein because of defective diacylglyceryl transferase. Defective modification of prolipoprotein in umpA mutants was confirmed by in vitro analysis of prolipoprotein modification activity.	challenge
acceptable	SHIFL:MXIC	2015-11-18 14:49:15 CST	GO:0050709 negative regulation of protein secretion (P)	PMID:19017268	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1	challenge

acceptable:2
unacceptable:6
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flagged:0

Annotations challenged by Avf5209

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