GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com


Jump to: navigation, search

CACAO Fall 2015

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableHUMAN:BAG32015-10-14 09:53:51 CDTGO:0005737 cytoplasm (C)PMID:25149536ECO:0000314 direct assay evidence used in manual assertion

Immunohistochemical analysis of BAG3 expression in Figure 1 A, E and F.

unacceptableECOLI:EX12015-11-02 10:46:00 CSTGO:0000175 3'-5'-exoribonuclease activity (F)PMID:4927675ECO:0000315 mutant phenotype evidence used in manual assertion

As shown in Table 3, the wildtype strain of the gene sbcB shows exonuclease activity. When the anitserum, that inhibits exonuclease activity, was added the exonuclease activity decreased proving the exonuclease activity of the crude lysates. To verify the sbcB gene activity, the gene was mutated, sbcB15 and when mutated the exonuclease activity decreased to show that the sbcB gene is responsible for exonuclease activity.

unacceptableSHIFL:Q83M612015-11-17 12:08:56 CSTGO:0004519 endonuclease activity (F)PMID:9133662ECO:0000314 direct assay evidence used in manual assertion

Both SbcC and SbcD polypeptides are required for the ATP-dependent double-strand exonuclease activity of SbcCD (Fig.3b) only the SbcD polypeptide is required for the ATP-independent single-strand endonuclease activity of SbcCD

unacceptableBUCAI:END12015-11-17 14:40:00 CSTGO:0004530 deoxyribonuclease I activity (F)PMID:366ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 shows strain 577, which carries the end-14 mutation, is deficient in the major endonuclease

unacceptableECOLX:TRAJ22015-11-17 16:32:35 CSTGO:0000746 conjugation (P)PMID:24723101ECO:0000314 direct assay evidence used in manual assertion

The expression of traJ was activated at low concentration of bile salts and decreased with increasing bile salt concentration. The expression patterns of traJ were corresponded with the conjugative transfer frequencies between S. typhimurium and e. coli.

unacceptableECOLI:DPO3A2015-11-18 09:28:08 CSTGO:0003887 DNA-directed DNA polymerase activity (F)PMID:16164549ECO:0000315 mutant phenotype evidence used in manual assertion

in Table 3 are the mutant DNA strains (dnaE486 and dnaE511) against the wildtype to show that the increasing mutations are caused by defects in the DNA polymerase III alpha subunit that are encoded by dnaE. The mutations of the dnaE prove to be responsible/involved in DNA polymerase activity.

acceptableECOLI:LGT2015-11-18 11:45:25 CSTGO:0008961 phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity (F)PMID:7896715ECO:0000315 mutant phenotype evidence used in manual assertion

These results (Fig. 1 and Table 1) show that E. coli umpA mutants (strains SK634, SK635, and SK636) were defective in diacylglyceryl modification of prolipoprotein because of defective diacylglyceryl transferase. Defective modification of prolipoprotein in umpA mutants was confirmed by in vitro analysis of prolipoprotein modification activity.

acceptableSHIFL:MXIC2015-11-18 14:49:15 CSTGO:0050709 negative regulation of protein secretion (P)PMID:19017268ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1



Annotations challenged by Avf5209

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Avf5209

What do the icons mean in the status column?

assessment icons.jpg