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PMID:4927675
| Citation |
Kushner, SR, Nagaishi, H, Templin, A and Clark, AJ (1971) Genetic recombination in Escherichia coli: the role of exonuclease I. Proc. Natl. Acad. Sci. U.S.A. 68:824-7 |
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| Abstract |
The indirect suppression of recB(-) and recB(-)recC(-) mutations by the sbcB(-) allele is caused by the loss of a nuclease active on denatured DNA. Results from enzyme purifications and studies with a specific antiserum demonstrate that the activity present in sbcB(+) strains, and lost in sbcB(-) strains, is exonuclease I. It is likely that sbcB is the structural gene for exonuclease I. The loss of exonuclease I activity restores the recombination proficiency of Escherichia coli cells that has been lost by mutations in the recB and/or recC genes. This indicates that in the absence of the recB-recC-determined enzyme, exonuclease I prevents recombination. Hypothetical pathways illustrating this conclusion are presented. |
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| Keywords |
Alleles; DNA; Deoxyribonucleases/isolation & purification; Deoxyribonucleases/physiology; Escherichia coli/enzymology; Genes; Immune Sera/pharmacology; Mutation; Recombination, Genetic; Transduction, Genetic |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000175: 3'-5'-exoribonuclease activity |
ECO:0000315: |
F |
As shown in Table 3, the wildtype strain of the gene sbcB shows exonuclease activity. When the anitserum, that inhibits exonuclease activity, was added the exonuclease activity decreased proving the exonuclease activity of the crude lysates. To verify the sbcB gene activity, the gene was mutated, sbcB15 and when mutated the exonuclease activity decreased to show that the sbcB gene is responsible for exonuclease activity. |
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Notes
See also
References
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