GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
ECOLI:DPO3A
Contents
Species (Taxon ID) | Escherichia coli (strain K12). (83333) | |
Gene Name(s) | dnaE (synonyms: polC) | |
Protein Name(s) | DNA polymerase III subunit alpha | |
External Links | ||
UniProt | P10443 | |
EMBL | M19334 U70214 U00096 AP009048 S52931 M96394 D49445 | |
PIR | C28390 | |
RefSeq | NP_414726.1 YP_488486.1 | |
PDB | 2HNH 2HQA 4GX8 4GX9 4JOM | |
PDBsum | 2HNH 2HQA 4GX8 4GX9 4JOM | |
ProteinModelPortal | P10443 | |
SMR | P10443 | |
DIP | DIP-9458N | |
IntAct | P10443 | |
MINT | MINT-1224103 | |
STRING | 511145.b0184 | |
BindingDB | P10443 | |
ChEMBL | CHEMBL4621 | |
PaxDb | P10443 | |
PRIDE | P10443 | |
EnsemblBacteria | AAC73295 BAA77859 | |
GeneID | 12933234 944877 | |
KEGG | ecj:Y75_p0180 eco:b0184 | |
PATRIC | 32115479 | |
EchoBASE | EB0234 | |
EcoGene | EG10238 | |
eggNOG | COG0587 | |
HOGENOM | HOG000021784 | |
InParanoid | P10443 | |
KO | K02337 | |
OMA | MKFPGYM | |
OrthoDB | EOG6CZQGR | |
PhylomeDB | P10443 | |
BioCyc | EcoCyc:EG10238-MONOMER ECOL316407:JW0179-MONOMER MetaCyc:EG10238-MONOMER | |
EvolutionaryTrace | P10443 | |
PRO | PR:P10443 | |
Proteomes | UP000000318 UP000000625 | |
Genevestigator | P10443 | |
GO | GO:0005737 GO:0044776 GO:0003677 GO:0003887 GO:0071897 GO:0006260 | |
Gene3D | 2.40.50.140 | |
InterPro | IPR011708 IPR029460 IPR012340 IPR004365 IPR004013 IPR003141 IPR016195 IPR004805 | |
Pfam | PF07733 PF14579 PF02811 PF01336 | |
SMART | SM00481 | |
SUPFAM | SSF89550 | |
TIGRFAMs | TIGR00594 |
Annotations
Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|---|---|
GO:0006261 |
DNA-dependent DNA replication |
ECO:0000314 |
P |
Table 1 |
complete | |||||
NOT|enables |
GO:0008408 |
3'-5' exonuclease activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0044776 |
DNA polymerase III, core complex |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
part_of |
GO:0005829 |
cytosol |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0071897 |
DNA biosynthetic process |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003887 |
P |
Seeded From UniProt |
complete | ||
involved_in |
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0008408 |
P |
Seeded From UniProt |
complete | ||
enables |
GO:0003676 |
nucleic acid binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003824 |
catalytic activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0006260 |
DNA replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0006260 |
DNA replication |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
GO:0003887 |
DNA-directed DNA polymerase activity |
ECO:0000315 |
F |
in Table 3 are the mutant DNA strains (dnaE486 and dnaE511) against the wildtype to show that the increasing mutations are caused by defects in the DNA polymerase III alpha subunit that are encoded by dnaE. The mutations of the dnaE prove to be responsible/involved in DNA polymerase activity. |
complete | |||||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Wickner, S & Hurwitz, J (1974) Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins. Proc. Natl. Acad. Sci. U.S.A. 71 4120-4 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Maki, H & Kornberg, A (1985) The polymerase subunit of DNA polymerase III of Escherichia coli. II. Purification of the alpha subunit, devoid of nuclease activities. J. Biol. Chem. 260 12987-92 PubMed GONUTS page
- ↑ McHenry, CS & Crow, W (1979) DNA polymerase III of Escherichia coli. Purification and identification of subunits. J. Biol. Chem. 254 1748-53 PubMed GONUTS page
- ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
- ↑ Banach-Orlowska, M et al. (2005) DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli. Mol. Microbiol. 58 61-70 PubMed GONUTS page