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ECOLI:DPO3A

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Species (Taxon ID) Escherichia coli (strain K12). (83333)
Gene Name(s) dnaE (synonyms: polC)
Protein Name(s) DNA polymerase III subunit alpha
External Links
UniProt P10443
EMBL M19334
U70214
U00096
AP009048
S52931
M96394
D49445
PIR C28390
RefSeq NP_414726.1
YP_488486.1
PDB 2HNH
2HQA
4GX8
4GX9
4JOM
PDBsum 2HNH
2HQA
4GX8
4GX9
4JOM
ProteinModelPortal P10443
SMR P10443
DIP DIP-9458N
IntAct P10443
MINT MINT-1224103
STRING 511145.b0184
BindingDB P10443
ChEMBL CHEMBL4621
PaxDb P10443
PRIDE P10443
EnsemblBacteria AAC73295
BAA77859
GeneID 12933234
944877
KEGG ecj:Y75_p0180
eco:b0184
PATRIC 32115479
EchoBASE EB0234
EcoGene EG10238
eggNOG COG0587
HOGENOM HOG000021784
InParanoid P10443
KO K02337
OMA MKFPGYM
OrthoDB EOG6CZQGR
PhylomeDB P10443
BioCyc EcoCyc:EG10238-MONOMER
ECOL316407:JW0179-MONOMER
MetaCyc:EG10238-MONOMER
EvolutionaryTrace P10443
PRO PR:P10443
Proteomes UP000000318
UP000000625
Genevestigator P10443
GO GO:0005737
GO:0044776
GO:0003677
GO:0003887
GO:0071897
GO:0006260
Gene3D 2.40.50.140
InterPro IPR011708
IPR029460
IPR012340
IPR004365
IPR004013
IPR003141
IPR016195
IPR004805
Pfam PF07733
PF14579
PF02811
PF01336
SMART SM00481
SUPFAM SSF89550
TIGRFAMs TIGR00594

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0006261

DNA-dependent DNA replication

PMID:4610569[1]

ECO:0000314

P

Table 1

complete

NOT|enables

GO:0008408

3'-5' exonuclease activity

PMID:2997151[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

PMID:2997151[2]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0003887

DNA-directed DNA polymerase activity

PMID:2997151[2]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0044776

DNA polymerase III, core complex

PMID:368075[3]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0005829

cytosol

PMID:18304323[4]

ECO:0000314

direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0071897

DNA biosynthetic process

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0003887

P

Seeded From UniProt

complete

involved_in

GO:0071897

DNA biosynthetic process

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0003887

P

Seeded From UniProt

complete

involved_in

GO:0071897

DNA biosynthetic process

GO_REF:0000108

ECO:0000366

evidence based on logical inference from automatic annotation used in automatic assertion

GO:0003887

P

Seeded From UniProt

complete

involved_in

GO:0090305

nucleic acid phosphodiester bond hydrolysis

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0008408

P

Seeded From UniProt

complete

enables

GO:0003676

nucleic acid binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004365

F

Seeded From UniProt

complete

enables

GO:0003824

catalytic activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004013
InterPro:IPR016195

F

Seeded From UniProt

complete

involved_in

GO:0006260

DNA replication

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR004805
InterPro:IPR011708

P

Seeded From UniProt

complete

enables

GO:0003887

DNA-directed DNA polymerase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:2.7.7.7

F

Seeded From UniProt

complete

enables

GO:0003887

DNA-directed DNA polymerase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0239

F

Seeded From UniProt

complete

part_of

GO:0005737

cytoplasm

GO_REF:0000037
GO_REF:0000039

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0963
UniProtKB-SubCell:SL-0086

C

Seeded From UniProt

complete

enables

GO:0016740

transferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

involved_in

GO:0006260

DNA replication

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0235

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

enables

GO:0016779

nucleotidyltransferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0548

F

Seeded From UniProt

complete

GO:0003887

DNA-directed DNA polymerase activity

PMID:16164549[5]

ECO:0000315

F

in Table 3 are the mutant DNA strains (dnaE486 and dnaE511) against the wildtype to show that the increasing mutations are caused by defects in the DNA polymerase III alpha subunit that are encoded by dnaE. The mutations of the dnaE prove to be responsible/involved in DNA polymerase activity.

complete
CACAO 11170

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Wickner, S & Hurwitz, J (1974) Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins. Proc. Natl. Acad. Sci. U.S.A. 71 4120-4 PubMed GONUTS page
  2. 2.0 2.1 2.2 Maki, H & Kornberg, A (1985) The polymerase subunit of DNA polymerase III of Escherichia coli. II. Purification of the alpha subunit, devoid of nuclease activities. J. Biol. Chem. 260 12987-92 PubMed GONUTS page
  3. McHenry, CS & Crow, W (1979) DNA polymerase III of Escherichia coli. Purification and identification of subunits. J. Biol. Chem. 254 1748-53 PubMed GONUTS page
  4. Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed GONUTS page
  5. Banach-Orlowska, M et al. (2005) DNA polymerase II as a fidelity factor in chromosomal DNA synthesis in Escherichia coli. Mol. Microbiol. 58 61-70 PubMed GONUTS page