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PMID:4610569

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Citation

Wickner, S and Hurwitz, J (1974) Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins. Proc. Natl. Acad. Sci. U.S.A. 71:4120-4

Abstract

The E. coli proteins that catalyze the conversion of varphiX174 single-stranded DNA to duplex DNA have now been purified extensively. The reaction depends on dnaB, dnaC(D), dnaE, and dnaG gene products, DNA elongation factors I and II, E. coli DNA binding protein, and two additional E. coli proteins, replication factors X and Y. DNA synthesis by these proteins requires varphiX174 viral DNA, dNTPs, Mg(+2), and ATP. The product synthesized is full-length linear varphiX174 DNA. The reaction has been resolved into two steps. The first step involves the interaction of ATP and varphiX174 DNA with dnaB and dnaC(D) gene products, E. coli DNA binding protein, and replication factors X and Y in the absence of dNTPs. Subsequent dNMP incorporation requires the addition of DNA polymerase III, DNA elongation factors I and II, dnaG gene product, and dNTPs.

Links

PubMed PMC434340

Keywords

Adenosine Triphosphate/metabolism; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Coliphages; DNA Nucleotidyltransferases/metabolism; DNA Replication; DNA Viruses; DNA, Single-Stranded/metabolism; DNA, Viral/biosynthesis; DNA, Viral/metabolism; Deoxyribonucleotides/metabolism; Escherichia coli/metabolism; Protein Binding

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:PRIA

GO:0006261: DNA-dependent DNA replication

ECO:0000314:

P

Table 1

complete

ECOLI:DNAB

GO:0006261: DNA-dependent DNA replication

ECO:0000314:

P

Table 1

complete

ECOLI:DNAC

GO:0006261: DNA-dependent DNA replication

ECO:0000314:

P

Table 1

complete

ECOLI:DPO3X

GO:0006261: DNA-dependent DNA replication

ECO:0000314:

P

Table 1

complete

ECOLI:PRIC

GO:0006261: DNA-dependent DNA replication

ECO:0000314:

P

Table 1

complete

ECOLI:DPO3A

GO:0006261: DNA-dependent DNA replication

ECO:0000314:

P

Table 1

complete


See also

References

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