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User:Alqaffasaa

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Phage Hunters Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
BPA50:AEPE2017-04-19 20:01:22 CDTGO:0016787 hydrolase activity (F)PMID:8577256ECO:0000247 sequence alignment evidence used in manual assertion

Fig.3 shows high similarity between ply118 and ply500 protein sequence. wither it's a identical residue or a conservative substitution, the two enzymes share the acceptable shared identity

challenge
flaggedPYRHO:RTCB2017-04-17 10:32:32 CDTGO:0008664 manganese ion binding (F)PMID:22949672ECO:0000315 mutant phenotype evidence used in manual assertion

in figure 5(A), activity assay shows how the wild type of RtcB ligates a linear RNA to circular RNA. RtcB assayed and found to ligates the 5'-hydroxyl and 2',3'-cyclic phosphate of a 21-nt RNA. the figure also compare the wild type to three other mutant of RtcB. the three mutants did not form a circular RNA, conforming the wild type function.

challenge
BPA50:AEPE2017-04-19 20:01:22 CDTGO:0016787 hydrolase activity (F)PMID:8577256ECO:0000314 direct assay evidence used in manual assertion

In fig.8, an optical density assay was performed on Listeria monocytogenes WSLC 1001. the assay shows a decrees in the optical density of L.monocytogenes cell wall after being exposed to PL118 (closed circles).

PL118 and PL500 have acceptable sequence similarity to assume they have the same lytic function. the control(open circles) shows no effect on the cell wall without the addition of PL118.

challenge

acceptable:0
unacceptable:0
requires_changes:0
flagged:1

Annotations challenged by Alqaffasaa

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptableCakate1,
Team Purple-A
9CAUD:H2D0G4GO:0016787 - hydrolase activity (F)PMID:22289622ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
acceptableCakate1,
Team Purple-A
9CAUD:R4JMG7GO:0008233 - peptidase activity (F)PMID:27637947ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
unacceptableIg4,
Team Green-A
9VIRU:A0A1B0VMI2GO:0016787 - hydrolase activity (F)PMID:4936722ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3
requireschangesStuitjj,
Team X-wing@Alliciousness
BPPH2:CAPSDGO:0019012 - virion (C)PMID:15837419ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

0 annotations fixed by Alqaffasaa

What do the icons mean in the status column?

assessment icons.jpg