User:Al271843

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	STRTR:E7AXS8	2012-03-27 12:29:04 CDT	GO:0004398 histidine decarboxylase activity (F)	PMID:22429258	ECO:0000270 expression pattern evidence used in manual assertion	Figure 2A shows that at the pH values 7.0, 6.2, and 5.5 the OD600 rapidly increased between 8 and 24 h of incubation reaching at 48 h the maximum values, corresponding to cell concentrations between 9.2 and 8.8 log CFU/mL. Also, in Figure in 3A, histamine rapidly accumulated at pH 7.0, 6.2, and 5.5 and a maximum concentration of about 350 mg/L was reached after 24 h. In both cases the production of histamine increased concomitantly with the cell number with a relatively small further rise during the stationary phase. In all cultures the maximum histamine levels were reached at the end of active growth. Histamine was detectable (10 to 55 mg/L) even when growth was strongly inhibited.The HdcA decarboxylase of this strain is very active since the exponential phase under different conditions characterizing the 1st step of the cheesemaking process. In addition, the enzyme is even more active outside the cells and its action is not significantly limited by the conditions found during the cheese ripening process. In this framework, the death and lysis of the starter cultures can be a factor which strengthens the risk of histamine accumulation during cheese ripening when temperature, pH, and NaCl concentration allow an intense activity of this HdcA enzyme.	challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Al271843

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Te211568, Team Robert Koch	LACCA:PTLCB	GO:0016310 - phosphorylation (P)	PMID:2125053	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 4

0 annotations fixed by Al271843

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	STAA8:LACR	2012-02-22 15:39:06 CST	GO:0006355 regulation of transcription, DNA-dependent (P)	PMID:2163387	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 6 shows that the introduction of the frameshift mutation causes premature termination of the lacR protein product resulting in a loss of repressor activity.	challenge
requireschanges	LACAC:Q5FI04	2012-03-09 10:03:46 CST	GO:0050082 maltose phosphorylase activity (F)	PMID:20713411	ECO:0000270 expression pattern evidence used in manual assertion	Fig. 2 shows a short loop 3, corresponding to LaMP His413–Glu415 in the (α/α)6-barrel catalytic domain of GH65 disaccharide phosphorylase, is identified to be a key determinant for specificity both in phosphorolysis and in regiospecific reverse phosphorolysis. Lactobacillus acidophilus NCFM maltose phosphorylase (LaMP) of the (α/α)6-barrel glycoside hydrolase family 65 (GH65) catalyses both phosphorolysis of maltose and formation of maltose by reverse phosphorolysis with β-glucose 1-phosphate and glucose as donor and acceptor, respectively.	challenge

acceptable:1
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Al271843

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Te211568, Team Robert Koch	LACCA:PTLCB	GO:0016310 - phosphorylation (P)	PMID:2125053	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 4

0 annotations fixed by Al271843