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PMID:2163387

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Citation

Oskouian, B and Stewart, GC (1990) Repression and catabolite repression of the lactose operon of Staphylococcus aureus. J. Bacteriol. 172:3804-12

Abstract

The lacR gene encodes the repressor of the lactose operon of S. aureus. The nucleotide sequence of this gene and the promoter-operator region of the operon are reported. The lacR gene encodes a protein with a molecular weight of 28,534. This protein was found to share sequence homology with the DeoR protein, the repressor of the E. coli deoxyribonucleotide operon. Directly and invertedly repeated sequences were found associated with the promoter for the structural genes of the operon. These sequences were examined by site-directed mutagenesis and found to be important in repressor binding and in the binding of a catabolite repressor. Evidence is presented in support of a model for catabolite repression of the operon which involves a negative-acting transcriptional regulator which binds to the promoter region of the operon and prevents transcription.

Links

PubMed PMC213359

Keywords

Amino Acid Sequence; Bacillus subtilis/genetics; Base Sequence; Chromosome Deletion; Cloning, Molecular; Conjugation, Genetic; DNA Transposable Elements; Escherichia coli/genetics; Genes, Bacterial; Genetic Techniques; Genotype; Lactose/metabolism; Molecular Sequence Data; Mutation; Oligonucleotide Probes; Operon; Repressor Proteins/genetics; Restriction Mapping; Sequence Homology, Nucleic Acid; Staphylococcus aureus/genetics; Transcription Factors/genetics

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

STAA8:LACR

GO:0006355: regulation of transcription, DNA-dependent

ECO:0000315:

P

Fig. 6 shows that the introduction of the frameshift mutation causes premature termination of the lacR protein product resulting in a loss of repressor activity.

complete
CACAO 3804

STAA8:LACR

involved_in

GO:0006355: regulation of transcription, DNA-templated

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete


See also

References

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