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Cacao
| GO:0045892 | negative regulation of transcription, DNA-templated | PMID:19703315 | ECO:0001819 IDA: Inferred from Direct Assay | Biological Process | Figure 5 shows the primer extension analysis of the sycO-ypkA-yopJ operon with and without CRP. A wild type with CRP and a strain without CRP were used. The WT shows no primer extension product while the stain without CRP shows product. The presence of the primer extension product in deleted CRP indicates mRNA presence. The lack of primer extension product in the WT indicates no mRNA presence. Because mRNA presence is high only in the deleted CRP strain, CRP is repressing transcription of the sycO-ypkA-yopJ operon. | complete | ||
| This annotation made on page: YERPE:A0A384L3J4 By: MGuldan (group Team Green A 2019) on 2019-04-10 13:52:10 CDT. | ||||||||
You must be logged in to challenge this annotation.
| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Challenge | KAtanasoff, Team Blue A 2019 | 2019-04-15 09:38:28 CDT | Your ECO term should be the child term "primer extension assay evidence used in manual assertion" (ECO:00055657). Also, I think the proper experimental evidence code should be IMP (Inferred from Mutant Phenotype) since the authors introduced a deletion mutation to knock out the crp gene and are comparing wild-type to Δcrp in the figure you cite. | 1 | ||||||||||||||||||||||
| Public Assessment | Ivanerill | 2019-04-20 04:46:37 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-04-18 16:30:43 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||||||
| Public Assessment | Ivanerill | 2019-04-18 10:22:44 CDT | Notes: "the stain" --> please revise | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-04-17 10:17:11 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged |
