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GO:0004146dihydrofolate reductase activityPMID:26147643ISS: Inferred from Sequence Similarity UniProtKB:P0ABQ4 F
This annotation made on page: 9CAUD:A0A0K2D000
By: E63 (group Team Blue A) on 2016-04-04 09:42:34 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeVumh,
Team Kendrick Jafars
2016-04-04 10:24:07 CDT

To use an ISS Transfer annotation, you must use GO_REF:0000100 as a reference.

1
ChallengeE63,
Team Blue A
2016-03-30 16:52:09 CDT

You do not have to use IGC in this situation. IGC is used when you cannot rely solely on homology. In this case, I can rely on homology because the e value that I obtained in BLAST is low enough that there is strong homology.

1
ChallengeChensi1,
Team Red A
2016-03-30 11:24:10 CDT

The accession# of homolog gene which used for transfer annotation should put in with/from.

1
ChallengeShireen2,
Team Red A
2016-03-30 09:53:28 CDT

since its a transfer annotation you should use the evidence code IGC.

0
Public
Assessment
Scaruso2016-05-09 13:54:35 CDT

This should be a transfer annotation using the SEA-PHAGES GO Ref (GO_REF 0000100) and ISS or IGC as the evidence code and the protein of interest's Uniprot ID(s) in the with/from field. Then you should have all of the information in this annotation put in the notes, including the PMID and your justification.

Unacceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Private
Assessment
Suzialeksander2016-05-04 14:11:12 CDTYou need to be an instructor to view these notes.Requires Changes
Publication
Notes
Public
Assessment
DanielRenfro2016-04-04 21:42:34 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004146 dihydrofolate reductase activity PMID:26147643 ISS: Inferred from Sequence Similarity UniProtKB:P0ABQ4 F Transfer annotation from gene page ECOLI:DYR.

With an E value of 2e-35, there is strong support for sequence similarity (accession #: 5CC9_A).||complete
CACAO 11421

on 9CAUD:A0A0K2D000
Flagged