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Category:Team Hot Cold

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableHYPSL:DMOACkting, Team Hot Cold2013-11-10 17:54:19 CSTGO:0018633 dimethyl sulfide monooxygenase activity (F)PMID:21216999ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows that Dimethylsulfide (DMS) is oxidized by DMS monooxygenase.

challenge
requireschangesCAMJJ:CDTACkting, Team Hot Cold2013-11-10 17:44:25 CSTGO:0071850 mitotic cell cycle arrest (P)PMID:12933829ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that CdtA subunit is involved in G2 arrest in HeLa cells.

challenge
unacceptableCAMJE:CH60Ckting, Team Hot Cold2013-11-10 16:02:33 CSTGO:0019724 B cell mediated immunity (P)PMID:10206714ECO:0000314 direct assay evidence used in manual assertion

Fig 7 shows that rCjCpn60-His (GroEL protein) had an immuno-reactivity against anti-HSP60 monoclonal antibody.

challenge
unacceptableCAMJE:CH60Ckting, Team Hot Cold2013-11-10 15:45:58 CSTGO:0009408 response to heat (P)PMID:10206714ECO:0000314 direct assay evidence used in manual assertion

Fig 6 shows that groESL mRNA expression increased significantly when C.jejuni cells were shifted to 48C.

challenge
updatedbyinstructorHYPSL:DMOACkting, Team Hot Cold2013-10-27 16:01:06 CDTGO:0018633 dimethyl sulfide monooxygenase activity (F)PMID:21216999ECO:0000314 direct assay evidence used in manual assertion

Fig 3

challenge
acceptableSULAC:Q4J9M3Dewinter, Team Hot Cold2013-10-27 09:32:59 CDTGO:0032450 maltose alpha-glucosidase activity (F)PMID:23396915ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows specific activity of MalA with various substrates

challenge
acceptable9BACI:D6CJK0Tgrittner, Team Hot Cold2013-10-13 11:48:26 CDTGO:0008863 formate dehydrogenase (NAD+) activity (F)PMID:21848698ECO:0000314 direct assay evidence used in manual assertion

Figure 4. figure showing the effects of pH and temperature on the BacFDH protein

challenge
unacceptable?Ckting, Team Hot Cold2013-10-11 12:47:52 CDTGO:0048518 positive regulation of biological process (Fig 4 shows the addition of YFP to luciferase-FMN assay increases the light intensity.)PMID:3480518IDA: Inferred from Direct Assay
challenge
updatedbyinstructorALIFS:LUXYCkting, Team Hot Cold2013-10-11 12:34:11 CDTGO:0008218 bioluminescence (P)PMID:3480518ECO:0000314 direct assay evidence used in manual assertion

Fig 4 shows the presence of YFP in luciferase-FMN oxidoreductase assay emits light at 484nm and 534nm.

challenge
updatedbyinstructorRHORT:GLNDCkting, Team Hot Cold2013-10-11 11:16:03 CDTGO:0045893 positive regulation of transcription, DNA-templated (P)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that no GlnJ protein was synthesized in glnD mutant R.rubrum

challenge
updatedbyinstructorLEGPH:Q5ZSD5Dewinter, Team Hot Cold2013-10-10 17:28:48 CDTGO:0052866 phosphatidylinositol phosphate phosphatase activity (F)PMID:23843460ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1

challenge
unacceptableRHILE:Q52856Tgrittner, Team Hot Cold2013-09-29 10:58:57 CDTGO:0010468 regulation of gene expression (P)PMID:23708137ECO:0000314 direct assay evidence used in manual assertion

Table 2 Shows that expression of pssA is effected by the presence of RosR phosphate and carbon by beta-galactosidase activity.

challenge
unacceptableRHORT:GLNDCkting, Team Hot Cold2013-09-28 15:30:19 CDTGO:0008773 [protein-PII] uridylyltransferase activity (F)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5 shows that no GlnJ protein was synthesized in glnD mutant R.rubrum.

challenge
acceptableRHORT:GLNDCkting, Team Hot Cold2013-09-28 14:55:59 CDTGO:0018177 protein uridylylation (P)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows the impact of glnD mutation on the ability of mutant R.rubrum to recover its nitrogenase activity via GlnB and GlnJ uridylylation after returning to light.

challenge
acceptableRHORT:GLNDCkting, Team Hot Cold2013-09-28 14:49:44 CDTGO:0090293 nitrogen catabolite regulation of transcription (P)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 shows that the presence of N-terminal region of GlnD allows glnD mutant R.rubrum to have nitrogenase activity via NifA activation.

challenge
unacceptableVIBF1:Q5E5B9Dewinter, Team Hot Cold2013-09-24 12:16:04 CDTGO:0004177 aminopeptidase activity (F)PMID:22636772ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3 shows the pepN mutant strains had delayed colonization of the squid light organ, which was corrected when pepN was expressed on a plasmid.

challenge
acceptablePSEHT:Q3IJB1Dewinter, Team Hot Cold2013-09-15 17:50:08 CDTGO:0044777 single-stranded DNA-binding protein complex (C)PMID:23953921ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows the PhaSSB protein/ssDNA complexes at various concentrations and lengths of ssDNA, indicating that a protein-substrate interaction is occurring.

challenge
updatedbyinstructorTHEKO:Q5JGC2Dewinter, Team Hot Cold2013-09-15 13:37:38 CDTGO:0008677 2-dehydropantoate 2-reductase activity (F)PMID:23941541ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows the kinetic activity of the isolated enzyme. The substrates it acts upon correlate with the proposed function of this enzyme.

challenge

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Pages in category "Team Hot Cold"

The following 4 pages are in this category, out of 4 total.


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