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RHORT:GLND
Contents
| Species (Taxon ID) | Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255). (269796) | |
| Gene Name(s) | glnD (ECO:0000255 with HAMAP-Rule:MF_00277) | |
| Protein Name(s) | Bifunctional uridylyltransferase/uridylyl-removing enzyme (ECO:0000255 with HAMAP-Rule:MF_00277)
UTase/UR (ECO:0000255 with HAMAP-Rule:MF_00277) Bifunctional [protein-PII] modification enzyme (ECO:0000255 with HAMAP-Rule:MF_00277) Bifunctional nitrogen sensor protein (ECO:0000255 with HAMAP-Rule:MF_00277) [Protein-PII] uridylyltransferase (ECO:0000255 with HAMAP-Rule:MF_00277) PII uridylyltransferase (ECO:0000255 with HAMAP-Rule:MF_00277) UTase (ECO:0000255 with HAMAP-Rule:MF_00277) [Protein-PII]-UMP uridylyl-removing enzyme (ECO:0000255 with HAMAP-Rule:MF_00277) UR (ECO:0000255 with HAMAP-Rule:MF_00277) | |
| External Links | ||
| UniProt | Q2RNG2 | |
| EMBL | CP000230 | |
| RefSeq | YP_428620.1 | |
| ProteinModelPortal | Q2RNG2 | |
| STRING | 269796.Rru_A3539 | |
| EnsemblBacteria | ABC24333 | |
| GeneID | 3836994 | |
| KEGG | rru:Rru_A3539 | |
| PATRIC | 23330876 | |
| eggNOG | COG2844 | |
| HOGENOM | HOG000261779 | |
| KO | K00990 | |
| OMA | STIGERV | |
| OrthoDB | EOG6CCH44 | |
| BioCyc | RRUB269796:GCN1-3602-MONOMER | |
| Proteomes | UP000001929 | |
| GO | GO:0008773 GO:0016597 GO:0008081 GO:0009399 GO:0006808 | |
| Gene3D | 1.10.3210.10 | |
| HAMAP | MF_00277 | |
| InterPro | IPR002912 IPR010043 IPR003607 IPR006674 IPR002934 IPR013546 | |
| Pfam | PF01842 PF08335 PF01966 PF01909 | |
| PIRSF | PIRSF006288 | |
| SMART | SM00471 | |
| TIGRFAMs | TIGR01693 | |
| PROSITE | PS51671 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0009399 |
nitrogen fixation |
ECO:0000315 |
P |
Table 1. |
complete | |||||
| GO:0008773 |
(protein-PII) uridylyltransferase activity |
PMID:PMC2876476 |
ECO:0000315 |
F |
Figure 5 shows mutations in the N-terminal domain significantly lowers UTase activity |
complete | ||||
| GO:0008773 |
(protein-PII) uridylyltransferase activity |
ECO:0000269 |
F |
Figure 7. |
complete | |||||
| GO:0008773 |
(protein-PII) uridylyltransferase activity |
ECO:0000269 |
F |
Fig. 1. Shows effect of divalent cations on uridylylation of PII proteins by GlnD using SDS-PAGE and Western Blot. |
complete | |||||
| GO:0090293 |
nitrogen catabolite regulation of transcription |
ECO:0000315 |
P |
Table 2 shows that the presence of N-terminal region of GlnD allows glnD mutant R.rubrum to have nitrogenase activity via NifA activation. |
complete | |||||
| GO:0018177 |
protein uridylylation |
ECO:0000315 |
P |
Table 3 shows the impact of glnD mutation on the ability of mutant R.rubrum to recover its nitrogenase activity via GlnB and GlnJ uridylylation after returning to light. |
complete | |||||
| GO:0008773 |
[protein-PII] uridylyltransferase activity |
ECO:0000315 |
F |
Fig 5 shows that no GlnJ protein was synthesized in glnD mutant R.rubrum. |
complete | |||||
| GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000315 |
P |
Figure 5 shows that no GlnJ protein was synthesized in glnD mutant R.rubrum |
complete | |||||
|
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0018177 |
protein uridylylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0090293 |
nitrogen catabolite regulation of transcription |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006807 |
nitrogen compound metabolic process |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008773 |
[protein-PII] uridylyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008773 |
[protein-PII] uridylyltransferase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006808 |
regulation of nitrogen utilization |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000035352 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0009399 |
nitrogen fixation |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000035352 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008773 |
[protein-PII] uridylyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000035352 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008081 |
phosphoric diester hydrolase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
UniRule:UR000035352 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009399 |
nitrogen fixation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003824 |
catalytic activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008152 |
metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Zhang, Y et al. (2010) Mutagenesis and functional characterization of the four domains of GlnD, a bifunctional nitrogen sensor protein. J. Bacteriol. 192 2711-21 PubMed GONUTS page
- ↑ Jonsson, A & Nordlund, S (2007) In vitro studies of the uridylylation of the three PII protein paralogs from Rhodospirillum rubrum: the transferase activity of R. rubrum GlnD is regulated by alpha-ketoglutarate and divalent cations but not by glutamine. J. Bacteriol. 189 3471-8 PubMed GONUTS page
- ↑ Teixeira, PF et al. (2008) Interaction of the signal transduction protein GlnJ with the cellular targets AmtB1, GlnE and GlnD in Rhodospirillum rubrum: dependence on manganese, 2-oxoglutarate and the ADP/ATP ratio. Microbiology (Reading, Engl.) 154 2336-47 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 4.6 Zhang, Y et al. (2005) GlnD is essential for NifA activation, NtrB/NtrC-regulated gene expression, and posttranslational regulation of nitrogenase activity in the photosynthetic, nitrogen-fixing bacterium Rhodospirillum rubrum. J. Bacteriol. 187 1254-65 PubMed GONUTS page
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