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PMID:21848698

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Citation

Ding, HT, Liu, DF, Li, ZL, Du, YQ, Xu, XH and Zhao, YH (2011) Characterization of a thermally stable and organic solvent-adaptative NAD+ -dependent formate dehydrogenase from Bacillus sp. F1. J. Appl. Microbiol. 111:1075-85

Abstract

To characterize a robust NAD(+) -dependent formate dehydrogenase firstly obtained from a nonmethylotroph, Bacillus sp. F1.

Links

PubMed Online version:10.1111/j.1365-2672.2011.05124.x

Keywords

Amino Acid Sequence; Bacillus/enzymology; Bacillus/genetics; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Cloning, Molecular; DNA, Bacterial/genetics; Enzyme Stability; Escherichia coli/metabolism; Formate Dehydrogenases/genetics; Formate Dehydrogenases/isolation & purification; Formate Dehydrogenases/metabolism; Half-Life; Hydrogen-Ion Concentration; Molecular Sequence Data; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Analysis, DNA; Solvents/chemistry; Substrate Specificity; Temperature

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

9BACI:D6CJK0

enables

GO:0008863: formate dehydrogenase (NAD+) activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

9BACI:D6CJK0

GO:0008863: formate dehydrogenase (NAD+) activity

ECO:0000314:

F

Figure 4. figure showing the effects of pH and temperature on the BacFDH protein

complete
CACAO 8860

See also

References

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