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User:Ckting

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableRHORT:GLND2013-09-28 14:49:44 CDTGO:0090293 nitrogen catabolite regulation of transcription (P)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 shows that the presence of N-terminal region of GlnD allows glnD mutant R.rubrum to have nitrogenase activity via NifA activation.

challenge
acceptableRHORT:GLND2013-09-28 14:55:59 CDTGO:0018177 protein uridylylation (P)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3 shows the impact of glnD mutation on the ability of mutant R.rubrum to recover its nitrogenase activity via GlnB and GlnJ uridylylation after returning to light.

challenge
unacceptableRHORT:GLND2013-09-28 15:30:19 CDTGO:0008773 [protein-PII] uridylyltransferase activity (F)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5 shows that no GlnJ protein was synthesized in glnD mutant R.rubrum.

challenge
updatedbyinstructorRHORT:GLND2013-10-11 11:16:03 CDTGO:0045893 positive regulation of transcription, DNA-templated (P)PMID:15687189ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows that no GlnJ protein was synthesized in glnD mutant R.rubrum

challenge
updatedbyinstructorALIFS:LUXY2013-10-11 12:34:11 CDTGO:0008218 bioluminescence (P)PMID:3480518ECO:0000314 direct assay evidence used in manual assertion

Fig 4 shows the presence of YFP in luciferase-FMN oxidoreductase assay emits light at 484nm and 534nm.

challenge
unacceptable?2013-10-11 12:47:52 CDTGO:0048518 positive regulation of biological process (Fig 4 shows the addition of YFP to luciferase-FMN assay increases the light intensity.)PMID:3480518IDA: Inferred from Direct Assay
challenge
updatedbyinstructorHYPSL:DMOA2013-10-27 16:01:06 CDTGO:0018633 dimethyl sulfide monooxygenase activity (F)PMID:21216999ECO:0000314 direct assay evidence used in manual assertion

Fig 3

challenge
unacceptableCAMJE:CH602013-11-10 15:45:58 CSTGO:0009408 response to heat (P)PMID:10206714ECO:0000314 direct assay evidence used in manual assertion

Fig 6 shows that groESL mRNA expression increased significantly when C.jejuni cells were shifted to 48C.

challenge
unacceptableCAMJE:CH602013-11-10 16:02:33 CSTGO:0019724 B cell mediated immunity (P)PMID:10206714ECO:0000314 direct assay evidence used in manual assertion

Fig 7 shows that rCjCpn60-His (GroEL protein) had an immuno-reactivity against anti-HSP60 monoclonal antibody.

challenge
requireschangesCAMJJ:CDTA2013-11-10 17:44:25 CSTGO:0071850 mitotic cell cycle arrest (P)PMID:12933829ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows that CdtA subunit is involved in G2 arrest in HeLa cells.

challenge
unacceptableHYPSL:DMOA2013-11-10 17:54:19 CSTGO:0018633 dimethyl sulfide monooxygenase activity (F)PMID:21216999ECO:0000314 direct assay evidence used in manual assertion

Fig 3 shows that Dimethylsulfide (DMS) is oxidized by DMS monooxygenase.

challenge

acceptable:2
unacceptable:5
requires_changes:1
flagged:0

Annotations challenged by Ckting

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesRiebefoy,
Team 4 peas in a pod
VIBHB:LUXOGO:0006355 - regulation of DNA-templated transcription (P)PMID:10027982ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2

0 annotations fixed by Ckting

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