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Category:Team Grapes of Staph II
|Status||Page||User||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|CLOPF:Q1PLH8||Ismailsalad, Team Grapes of Staph II||2017-04-09 17:58:52 CDT||GO:0016020 membrane (C)||PMID:22150951||ECO:0000314 direct assay evidence used in manual assertion|
TcpC was detected in the membrane fraction of the wild-type strain and a complemented tcpC mutant, but was absent in the tcpC mutant carrying the shuttle vector control (Fig. 3).
|ECOLI:TRAD1||Ismailsalad, Team Grapes of Staph II||2017-04-08 19:19:04 CDT||GO:0009291 unidirectional conjugation (P)||PMID:18717787||ECO:0000315 mutant phenotype evidence used in manual assertion|
To understand the importance of the C-terminal tail of TraD for conjugation, we tested the ability of TraD mutants to complement conjugation of a plasmid derived from F, pOX38-D411, in which traD is knocked out (Table 2). Deletion of the C-terminal 141 amino acids of TraD (TraD576*) resulted in a 104-fold decrease in conjugation efficiency, compared with wild-type TraD, consistent with previous results (Sastre et al., 1998). This deletion removes the entire C-terminal tail but leaves intact the ATPase domain. Deletion of just the C-terminal eight amino acids (TraD709*) resulted in a 103-fold decrease in conjugation compared with the wild-type control, indicating that this region is critical for the normal function of the C-terminal tail in initiating contact with TraM.
|ENTFL:A0A140UHJ9||Annaerickson, Team Grapes of Staph II||2017-04-05 11:49:37 CDT||GO:0000746 conjugation (P)||PMID:27103580||ECO:0000315 mutant phenotype evidence used in manual assertion|
Supplementary Table 3 and section "TraH is an essential component in pIP501-mediated conjugation."
Although the paper didn't use the Enterococcus faecalis strain EnGen0234, the Protein Data Bank accession code mentioned in the paper matches the UniProt ID for the EnGen strain. The GO term is appropriate because a mutant strain with a deletion of the traH gene did not conjugate at a level that could be detected by the assay used. However, when the same strain received a plasmid containing a functional copy of the gene, conjugation proceeded at a normal rate.
|SALTI:Q9L5M5||Shelbywunderlich, Team Grapes of Staph II||2017-04-05 11:44:14 CDT||GO:0016020 membrane (C)||PMID:17259614||ECO:0000314 direct assay evidence used in manual assertion|
Figure 3a: TraJ-AC18 localizes to the cellular membrane
Salmonella typhi R27; AAF69955.1/TraJ
|ECOLX:TRAG4||Shelbywunderlich, Team Grapes of Staph II||2017-04-04 11:17:36 CDT||GO:0016020 membrane (C)||PMID:11735369||ECO:0000314 direct assay evidence used in manual assertion|
Figure 5b. Localization of TraG and TraN. SF, soluble fraction concentrated to match the concentration of the membrane fraction; IM , inner membrane fraction; OM, outer membrane fiction. TraG was detected in both the outer and inner fractions although more was seen in the outer membrane fraction than in the inner membrane fraction.
|ENTFL:Q8L1D1||Shelbywunderlich, Team Grapes of Staph II||2017-03-21 10:41:34 CDT||GO:0009291 unidirectional conjugation (P)||PMID:23913323||ECO:0000315 mutant phenotype evidence used in manual assertion|
4th paragraph of the initial section of the results: "Transfer rates of the traG deletion mutant were below the detection limit of the assay (<3.5 × 10−9 ± 2.4 × 10−9, mean value of three independent assays), whereas transfer rates with the isogenic strain E. faecalis OG1RF(pIP501) were at least 20,000 times higher and in the expected range for pIP501 of 7.6 × 10−5 ± 5.7 × 10−5 transconjugants per recipient cell (mean value of three independent assays)."