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User:Shelbywunderlich

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CACAO Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorENTFL:Q8L1D12017-03-21 10:41:34 CDTGO:0009291 unidirectional conjugation (P)PMID:23913323ECO:0000315 mutant phenotype evidence used in manual assertion

4th paragraph of the initial section of the results: "Transfer rates of the traG deletion mutant were below the detection limit of the assay (<3.5 × 10−9 ± 2.4 × 10−9, mean value of three independent assays), whereas transfer rates with the isogenic strain E. faecalis OG1RF(pIP501) were at least 20,000 times higher and in the expected range for pIP501 of 7.6 × 10−5 ± 5.7 × 10−5 transconjugants per recipient cell (mean value of three independent assays)."

challenge
unacceptableECOLX:TRAG42017-04-04 11:17:36 CDTGO:0016020 membrane (C)PMID:11735369ECO:0000314 direct assay evidence used in manual assertion

Figure 5b. Localization of TraG and TraN. SF, soluble fraction concentrated to match the concentration of the membrane fraction; IM , inner membrane fraction; OM, outer membrane fiction. TraG was detected in both the outer and inner fractions although more was seen in the outer membrane fraction than in the inner membrane fraction.

challenge
updatedbyinstructorSALTI:Q9L5M52017-04-05 11:44:14 CDTGO:0016020 membrane (C)PMID:17259614ECO:0000314 direct assay evidence used in manual assertion

Figure 3a: TraJ-AC18 localizes to the cellular membrane

Salmonella typhi R27; AAF69955.1/TraJ

challenge

acceptable:0
unacceptable:1
requires_changes:0
flagged:0

Annotations challenged by Shelbywunderlich

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