ECOLI:TRAD1

Species (Taxon ID)	Escherichia coli (strain K12). (83333)
Gene Name(s)	traD
Protein Name(s)	Coupling protein TraD DNA transport protein TraD
External Links
UniProt	P09130
EMBL	M29254 U01159 AJ011707 AP001918 AF550679 X06915 X57428 X57431 M54796
PIR	JS0293
RefSeq	NP_061481.1 NP_862947.1 WP_000009350.1 YP_009060132.1 YP_009062862.1 YP_009068320.1 YP_009070586.1
PDB	3D8A
PDBsum	3D8A
ProteinModelPortal	P09130
SMR	P09130
DIP	DIP-27652N
PRIDE	P09130
GeneID	1263585 1446510 20466888 20468912 20491450 20492654
PATRIC	fig|83333.107.peg.610
EvolutionaryTrace	P09130
PRO	PR:P09130
GO	GO:0016021 GO:0005886 GO:0005524 GO:0000746
InterPro	IPR027417 IPR014128 IPR019476 IPR022585
Pfam	PF12615 PF10412
SUPFAM	SSF52540
TIGRFAMs	TIGR02759

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
involved_in	GO:0000746	conjugation	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR014128	P		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C		Seeded From UniProt	complete
part_of	GO:0005886	plasma membrane	GO_REF:0000037 GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1003 UniProtKB-KW:KW-0997 UniProtKB-SubCell:SL-0037	C		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
involved_in	GO:0000746	conjugation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0184	P		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
	GO:0009291	unidirectional conjugation	PMID:18717787	ECO:0000315			P		Table 2: To understand the importance of the C-terminal tail of TraD for conjugation, we tested the ability of TraD mutants to complement conjugation of a plasmid derived from F, pOX38-D411, in which traD is knocked out (Table 2). Deletion of the C-terminal 141 amino acids of TraD (TraD576*) resulted in a 104-fold decrease in conjugation efficiency, compared with wild-type TraD, consistent with previous results (Sastre et al., 1998). This deletion removes the entire C-terminal tail but leaves intact the ATPase domain. Deletion of just the C-terminal eight amino acids (TraD709*) resulted in a 103-fold decrease in conjugation compared with the wild-type control, indicating that this region is critical for the normal function of the C-terminal tail in initiating contact with TraM. TRAD1_ECOLI=BAA97972	complete CACAO 12510
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References

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