CACAO Spring 2017
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| updatedbyinstructor | ECOLI:TRAD1 | 2017-04-08 19:19:04 CDT | GO:0009291 unidirectional conjugation (P) | PMID:18717787 | ECO:0000315 mutant phenotype evidence used in manual assertion | Table 2:
To understand the importance of the C-terminal tail of TraD for conjugation, we tested the ability of TraD mutants to complement conjugation of a plasmid derived from F, pOX38-D411, in which traD is knocked out (Table 2). Deletion of the C-terminal 141 amino acids of TraD (TraD576*) resulted in a 104-fold decrease in conjugation efficiency, compared with wild-type TraD, consistent with previous results (Sastre et al., 1998). This deletion removes the entire C-terminal tail but leaves intact the ATPase domain. Deletion of just the C-terminal eight amino acids (TraD709*) resulted in a 103-fold decrease in conjugation compared with the wild-type control, indicating that this region is critical for the normal function of the C-terminal tail in initiating contact with TraM.
TRAD1_ECOLI=BAA97972
| challenge |
| updatedbyinstructor | CLOPF:Q1PLH8 | 2017-04-09 17:58:52 CDT | GO:0016020 membrane (C) | PMID:22150951 | ECO:0000314 direct assay evidence used in manual assertion | TcpC was detected in the membrane fraction of the wild-type strain and a complemented tcpC mutant, but was absent in the tcpC mutant carrying the shuttle vector control (Fig. 3).
Clostridium perfringens
| challenge |
acceptable:0
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by Ismailsalad
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Ismailsalad
What do the icons mean in the status column?
