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YEAST:WHI3
Contents
| Species (Taxon ID) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292) | |
| Gene Name(s) | WHI3 | |
| Protein Name(s) | Protein WHI3 | |
| External Links | ||
| UniProt | P34761 | |
| EMBL | U01095 X78898 Z71473 BK006947 | |
| PIR | S50734 | |
| RefSeq | NP_014202.1 | |
| ProteinModelPortal | P34761 | |
| SMR | P34761 | |
| BioGrid | 35636 | |
| IntAct | P34761 | |
| MINT | MINT-4500279 | |
| STRING | 4932.YNL197C | |
| MaxQB | P34761 | |
| PaxDb | P34761 | |
| PeptideAtlas | P34761 | |
| EnsemblFungi | [example_ID YNL197C] | |
| GeneID | 855524 | |
| KEGG | sce:YNL197C | |
| CYGD | YNL197c | |
| SGD | S000005141 | |
| eggNOG | NOG255497 | |
| GeneTree | ENSGT00530000067542 | |
| HOGENOM | HOG000074699 | |
| InParanoid | P34761 | |
| OMA | VRGPNSR | |
| OrthoDB | EOG7PP5GG | |
| BioCyc | YEAST:G3O-33207-MONOMER | |
| NextBio | 979559 | |
| Proteomes | UP000002311 | |
| Genevestigator | P34761 | |
| GO | GO:0005737 GO:0003729 GO:0000166 GO:0007049 GO:0051220 GO:0001403 GO:0061157 GO:0007124 GO:0008361 GO:0061013 | |
| Gene3D | 3.30.70.330 | |
| InterPro | IPR012677 IPR000504 | |
| Pfam | PF00076 | |
| SMART | SM00360 | |
| PROSITE | PS50102 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0071554 |
cell wall organization or biogenesis |
ECO:0000315 |
P |
Figure 4 demonstrates that in cell walls that are deficient of WHI3 protein, significant deterioration occurs. In Figure 4, WHI3 deficient cells, as well as other mutants and controls, were treated with cell wall inhibitors resulting in horrible degradation in those cells without WHI3. The degradation in cells missing WHI3 can affect development and even survival of the cell. Cells with the protein were relatively unscathed by the cell wall inhibitors. |
complete | |||||
| GO:0045901 |
positive regulation of translational elongation |
ECO:0000315 |
P |
Fig. 1B, 2 - protein:mRNA ratio decreases in whi3delta mutant compared to wildtype even though RNA level remains constant - suggests Whi3 positively regulates translation Fig. 3 - wildtype and whi3delta mutant supplemented with inhibitors of translational elongation, growth of mutant more adversely affected than growth of wildtype - suggests Whi3 affects translation elongation specifically |
complete | |||||
| GO:0045793 |
positive regulation of cell size |
ECO:0000315 |
P |
Fig 1A - cell size decreases in whi3delta mutant |
complete | |||||
| GO:0010811 |
positive regulation of cell-substrate adhesion |
ECO:0000315 |
P |
Fig 1b: wildtype and mutant (didn't express Whi3) yeast both grew on plate. Mutant yeast washed off the plate, wildtype yeast stuck. |
complete | |||||
| GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000315 |
P |
Fig 1a: expression of Whi3 resulted in higher FLO11 RNA levels |
complete | |||||
| GO:0045727 |
positive regulation of translation |
ECO:0000315 |
P |
Fig 3a: Levels of Yak1-HA protein and YAK1-HA RNA were measured in wildtype and mutant (doesn't express Whi3) yeast - RNA levels remained constant in wildtype and mutant but protein levels decreased in mutant |
complete | |||||
| GO:0032106 |
positive regulation of response to extracellular stimulus |
ECO:0000315 |
P |
Fig 4a: Glucose starvation in wildtype led to increased levels of FLO11 RNA. Mutants without whi3 did not have increased levels of FLO11. |
complete | |||||
|
involved_in |
GO:0007089 |
traversing start control point of mitotic cell cycle |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007089 |
traversing start control point of mitotic cell cycle |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
SGD:S000000038 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0008361 |
regulation of cell size |
ECO:0007001 |
high throughput mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0010494 |
cytoplasmic stress granule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010811 |
positive regulation of cell-substrate adhesion |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032106 |
positive regulation of response to extracellular stimulus |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045727 |
positive regulation of translation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071554 |
cell wall organization or biogenesis |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045793 |
positive regulation of cell size |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045901 |
positive regulation of translational elongation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0061157 |
mRNA destabilization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
has_direct_input:(SGD:S000000038) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0061013 |
regulation of mRNA catabolic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051220 |
cytoplasmic sequestering of protein |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0010494 |
cytoplasmic stress granule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
exists_during:(GO:0034605) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0010494 |
cytoplasmic stress granule |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
exists_during:(GO:0034605) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0007124 |
pseudohyphal growth |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007124 |
pseudohyphal growth |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003729 |
mRNA binding |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
has_direct_input:(SGD:S000000038) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003729 |
mRNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
has_direct_input:(SGD:S000000038) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003729 |
mRNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
has_direct_input:(SGD:S000000038) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0001403 |
invasive growth in response to glucose limitation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0000932 |
P-body |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
exists_during:(GO:0042149) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0035614 |
snRNA stem-loop binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0003449 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0030619 |
U1 snRNA binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0003449 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005685 |
U1 snRNP |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000052389 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000398 |
mRNA splicing, via spliceosome |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000827569 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003676 |
nucleic acid binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007049 |
cell cycle |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Colomina, N et al. (2008) Whi3, a developmental regulator of budding yeast, binds a large set of mRNAs functionally related to the endoplasmic reticulum. J. Biol. Chem. 283 28670-9 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 Schladebeck, S & Mösch, HU (2013) The RNA-binding protein Whi3 is a key regulator of developmental signaling and ploidy in Saccharomyces cerevisiae. Genetics 195 73-86 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 Malcher, M et al. (2011) The Yak1 protein kinase lies at the center of a regulatory cascade affecting adhesive growth and stress resistance in Saccharomyces cerevisiae. Genetics 187 717-30 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 4.3 4.4 Garí, E et al. (2001) Whi3 binds the mRNA of the G1 cyclin CLN3 to modulate cell fate in budding yeast. Genes Dev. 15 2803-8 PubMed GONUTS page
- ↑ Jorgensen, P et al. (2002) Systematic identification of pathways that couple cell growth and division in yeast. Science 297 395-400 PubMed GONUTS page
- ↑ Jain, S et al. (2016) ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 164 487-98 PubMed GONUTS page
- ↑ 7.0 7.1 7.2 7.3 Cai, Y & Futcher, B (2013) Effects of the yeast RNA-binding protein Whi3 on the half-life and abundance of CLN3 mRNA and other targets. PLoS ONE 8 e84630 PubMed GONUTS page
- ↑ 8.0 8.1 Holmes, KJ et al. (2013) Whi3, an S. cerevisiae RNA-binding protein, is a component of stress granules that regulates levels of its target mRNAs. PLoS ONE 8 e84060 PubMed GONUTS page
- ↑ 9.0 9.1 9.2 Wang, H et al. (2004) Recruitment of Cdc28 by Whi3 restricts nuclear accumulation of the G1 cyclin-Cdk complex to late G1. EMBO J. 23 180-90 PubMed GONUTS page
- ↑ Mösch, HU & Fink, GR (1997) Dissection of filamentous growth by transposon mutagenesis in Saccharomyces cerevisiae. Genetics 145 671-84 PubMed GONUTS page
- ↑ 11.0 11.1 11.2 11.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
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- GO:0000932 ! P-body
- GO:0045901 ! positive regulation of translational elongation
- GO:0045893 ! positive regulation of DNA-templated transcription
- GO:0045793 ! positive regulation of cell size
- GO:0010811 ! positive regulation of cell-substrate adhesion
- GO:0045727 ! positive regulation of translation
- GO:0007124 ! pseudohyphal growth
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