PMID:18667435

Citation	Colomina, N, Ferrezuelo, F, Wang, H, Aldea, M and Garí, E (2008) Whi3, a developmental regulator of budding yeast, binds a large set of mRNAs functionally related to the endoplasmic reticulum. J. Biol. Chem. 283:28670-9
Abstract	Whi3 is an RNA-binding protein associated with the endoplasmic reticulum (ER) that binds the CLN3 mRNA and plays a key role in the efficient retention of cyclin Cln3 at the ER. In the present work, we have identified new Whi3-associated mRNAs by a genomic approach. A large and significant number of these Whi3 targets encode for membrane and exocytic proteins involved in processes such as transport and cell wall biogenesis. Consistent with the genomic data, we have observed that cell wall integrity is compromised in Whi3-deficient cells and found strong genetic interactions between WHI3 and the cell integrity pathway. Whi3-associated mRNAs are enriched in clusters of the tetranucleotide GCAU, and mutation of the GCAU clusters in the CLN3 mRNA caused a reduction in its association to Whi3, suggesting that these sequences may act as cis-determinants for binding. Our data suggest that Whi3 is involved in the regulation and/or localization of a large subset of mRNAs functionally related to the ER and, since it is important for different molecular processes such as cytoplasmic retention or exocytic traffic of proteins, we propose that Whi3 is a general modulator of protein fate in budding yeast.
Links	PubMed PMC2661415 Online version:10.1074/jbc.M804604200
Keywords	Cytoplasm/metabolism; Endoplasmic Reticulum/metabolism; Exocytosis; Gene Expression Regulation, Fungal; Genome, Fungal; Models, Biological; Multigene Family; Mutation; Oligonucleotide Array Sequence Analysis; Protein Binding; RNA, Messenger/metabolism; RNA-Binding Proteins/metabolism; RNA-Binding Proteins/physiology; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Saccharomycetales/metabolism; Temperature
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
YEAST:WHI3		GO:0071554: cell wall organization or biogenesis	ECO:0000315:		P		Figure 4 demonstrates that in cell walls that are deficient of WHI3 protein, significant deterioration occurs. In Figure 4, WHI3 deficient cells, as well as other mutants and controls, were treated with cell wall inhibitors resulting in horrible degradation in those cells without WHI3. The degradation in cells missing WHI3 can affect development and even survival of the cell. Cells with the protein were relatively unscathed by the cell wall inhibitors.	complete CACAO 9864
YEAST:WHI3	involved_in	GO:0071554: cell wall organization or biogenesis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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