YEAST:SIR4

Species (Taxon ID)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292)
Gene Name(s)	SIR4 (synonyms: ASD1, STE9, UTH2)
Protein Name(s)	Regulatory protein SIR4 Silent information regulator 4
External Links
UniProt	P11978
EMBL	M37249 U13239 Z48612 BK006938
PIR	A29360
RefSeq	NP_010513.1
PDB	1NYH 1PL5 4IAO
PDBsum	1NYH 1PL5 4IAO
ProteinModelPortal	P11978
SMR	P11978
BioGrid	32279
DIP	DIP-33N
IntAct	P11978
MINT	MINT-622754
STRING	4932.YDR227W
MaxQB	P11978
PaxDb	P11978
PeptideAtlas	P11978
EnsemblFungi	[example_ID YDR227W]
GeneID	851813
KEGG	sce:YDR227W
CYGD	YDR227w
SGD	S000002635
eggNOG	NOG38871
InParanoid	P11978
KO	K11123
OMA	TNDICSV
OrthoDB	EOG70GMTK
BioCyc	YEAST:G3O-29806-MONOMER
EvolutionaryTrace	P11978
NextBio	969671
Proteomes	UP000002311
ExpressionAtlas	P11978
Genevestigator	P11978
GO	GO:0005677 GO:0000784 GO:0005724 GO:0003690 GO:0031491 GO:0006342 GO:0001308 GO:0006351

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0007569	cell aging	PMID:10521401^[1]	ECO:0000315			P		Fig.1(A)demonstrates that disruption of sir4 resulted in a 20 % decrease in mean life span.	complete
	GO:0031453	positive regulation of heterochromatin assembly	PMID:26587833^[2]	ECO:0000314			P		Selection of one, two or four low-copy centromeric plasmids containing SIR4 in zygotes increased the amount of Sir4 protein in these cells and significantly increased the speed of establishment in the single cell assay(Figure2B-D and 3)	complete CACAO 11474
	GO:0035389	establishment of chromatin silencing at silent mating-type cassette	PMID:26587833^[2]	ECO:0000315			P		Selection of one, two or four low-copy centromeric plasmids containing SIR4 in zygotes increased the amount of Sir4 protein in these cells and significantly increased the speed of establishment in the single cell assay(Figure 2B-D and 3)	complete CACAO 11728
involved_in	GO:0097695	establishment of protein-containing complex localization to telomere	PMID:29290466^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	has_input:(ComplexPortal:CPX-3298)	Seeded From UniProt	complete
involved_in	GO:0097695	establishment of protein-containing complex localization to telomere	PMID:26218225^[4]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	has_input:(GO:0005697)	Seeded From UniProt	complete
involved_in	GO:0034398	telomere tethering at nuclear periphery	PMID:26399229^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0000784	nuclear chromosome, telomeric region	PMID:27122604^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		C	adjacent_to:(GO:0034399)	Seeded From UniProt	complete
involved_in	GO:0034398	telomere tethering at nuclear periphery	PMID:27122604^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0099114	chromatin silencing at subtelomere	PMID:26587833^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0031453	positive regulation of heterochromatin assembly	PMID:26587833^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0035389	establishment of chromatin silencing at silent mating-type cassette	PMID:26587833^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0032947	protein-containing complex scaffold activity	PMID:12080091^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		F	has_input:(GO:0005677)	Seeded From UniProt	complete
involved_in	GO:0006303	double-strand break repair via nonhomologous end joining	PMID:9501103^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006348	chromatin silencing at telomere	PMID:9501103^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006348	chromatin silencing at telomere	PMID:1913809^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0030466	chromatin silencing at silent mating-type cassette	PMID:3297920^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0003690	double-stranded DNA binding	PMID:22654676^[11]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0003690	double-stranded DNA binding	PMID:22654676^[11]	ECO:0000315	mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0030466	chromatin silencing at silent mating-type cassette	PMID:22654676^[11]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000004897	P		Seeded From UniProt	complete
involved_in	GO:0006348	chromatin silencing at telomere	PMID:22654676^[11]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0031491	nucleosome binding	PMID:19217406^[12]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0030466	chromatin silencing at silent mating-type cassette	PMID:22654676^[11]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0005724	nuclear telomeric heterochromatin	PMID:9000052^[13]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0005677	chromatin silencing complex	PMID:9122169^[14]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
enables	GO:0003690	double-stranded DNA binding	PMID:19217406^[12]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0001308	negative regulation of chromatin silencing involved in replicative cell aging	PMID:9150138^[15]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0000784	nuclear chromosome, telomeric region	PMID:9710643^[16]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0099115	chromosome, subtelomeric region	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0099114	C		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0539 UniProtKB-SubCell:SL-0191	C		Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ Kaeberlein, M et al. (1999) The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev. 13 2570-80 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 Larin, ML et al. (2015) Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin. PLoS Genet. 11 e1005425 PubMed GONUTS page
↑ Chen, H et al. (2018) Structural Insights into Yeast Telomerase Recruitment to Telomeres. Cell 172 331-343.e13 PubMed GONUTS page
↑ Hass, EP & Zappulla, DC (2015) The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae. Elife 4 PubMed GONUTS page
↑ Guidi, M et al. (2015) Spatial reorganization of telomeres in long-lived quiescent cells. Genome Biol. 16 206 PubMed GONUTS page
↑ ^6.0 ^6.1 Laporte, D et al. (2016) Quiescent Saccharomyces cerevisiae forms telomere hyperclusters at the nuclear membrane vicinity through a multifaceted mechanism involving Esc1, the Sir complex, and chromatin condensation. Mol. Biol. Cell 27 1875-84 PubMed GONUTS page
↑ Luo, K et al. (2002) Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast. Genes Dev. 16 1528-39 PubMed GONUTS page
↑ ^8.0 ^8.1 Boulton, SJ & Jackson, SP (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17 1819-28 PubMed GONUTS page
↑ Aparicio, OM et al. (1991) Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66 1279-87 PubMed GONUTS page
↑ Rine, J & Herskowitz, I (1987) Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae. Genetics 116 9-22 PubMed GONUTS page
↑ ^11.0 ^11.1 ^11.2 ^11.3 ^11.4 Kueng, S et al. (2012) Regulating repression: roles for the sir4 N-terminus in linker DNA protection and stabilization of epigenetic states. PLoS Genet. 8 e1002727 PubMed GONUTS page
↑ ^12.0 ^12.1 Martino, F et al. (2009) Reconstitution of yeast silent chromatin: multiple contact sites and O-AADPR binding load SIR complexes onto nucleosomes in vitro. Mol. Cell 33 323-34 PubMed GONUTS page
↑ Strahl-Bolsinger, S et al. (1997) SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. Genes Dev. 11 83-93 PubMed GONUTS page
↑ Moazed, D et al. (1997) Silent information regulator protein complexes in Saccharomyces cerevisiae: a SIR2/SIR4 complex and evidence for a regulatory domain in SIR4 that inhibits its interaction with SIR3. Proc. Natl. Acad. Sci. U.S.A. 94 2186-91 PubMed GONUTS page
↑ Kennedy, BK et al. (1997) Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell 89 381-91 PubMed GONUTS page
↑ Bourns, BD et al. (1998) Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo. Mol. Cell. Biol. 18 5600-8 PubMed GONUTS page

[PMID:10521401-1] Kaeberlein, M et al. (1999) The SIR2/3/4 complex and SIR2 alone promote longevity in Saccharomyces cerevisiae by two different mechanisms. Genes Dev. 13 2570-80 PubMed GONUTS page

[PMID:26587833-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 Larin, ML et al. (2015) Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin. PLoS Genet. 11 e1005425 PubMed GONUTS page

[PMID:29290466-3] Chen, H et al. (2018) Structural Insights into Yeast Telomerase Recruitment to Telomeres. Cell 172 331-343.e13 PubMed GONUTS page

[PMID:26218225-4] Hass, EP & Zappulla, DC (2015) The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae. Elife 4 PubMed GONUTS page

[PMID:26399229-5] Guidi, M et al. (2015) Spatial reorganization of telomeres in long-lived quiescent cells. Genome Biol. 16 206 PubMed GONUTS page

[PMID:27122604-6] 6.0 ^6.1 Laporte, D et al. (2016) Quiescent Saccharomyces cerevisiae forms telomere hyperclusters at the nuclear membrane vicinity through a multifaceted mechanism involving Esc1, the Sir complex, and chromatin condensation. Mol. Biol. Cell 27 1875-84 PubMed GONUTS page

[PMID:12080091-7] Luo, K et al. (2002) Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast. Genes Dev. 16 1528-39 PubMed GONUTS page

[PMID:9501103-8] 8.0 ^8.1 Boulton, SJ & Jackson, SP (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17 1819-28 PubMed GONUTS page

[PMID:1913809-9] Aparicio, OM et al. (1991) Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66 1279-87 PubMed GONUTS page

[PMID:3297920-10] Rine, J & Herskowitz, I (1987) Four genes responsible for a position effect on expression from HML and HMR in Saccharomyces cerevisiae. Genetics 116 9-22 PubMed GONUTS page

[PMID:22654676-11] 11.0 ^11.1 ^11.2 ^11.3 ^11.4 Kueng, S et al. (2012) Regulating repression: roles for the sir4 N-terminus in linker DNA protection and stabilization of epigenetic states. PLoS Genet. 8 e1002727 PubMed GONUTS page

[PMID:19217406-12] 12.0 ^12.1 Martino, F et al. (2009) Reconstitution of yeast silent chromatin: multiple contact sites and O-AADPR binding load SIR complexes onto nucleosomes in vitro. Mol. Cell 33 323-34 PubMed GONUTS page

[PMID:9000052-13] Strahl-Bolsinger, S et al. (1997) SIR2 and SIR4 interactions differ in core and extended telomeric heterochromatin in yeast. Genes Dev. 11 83-93 PubMed GONUTS page

[PMID:9122169-14] Moazed, D et al. (1997) Silent information regulator protein complexes in Saccharomyces cerevisiae: a SIR2/SIR4 complex and evidence for a regulatory domain in SIR4 that inhibits its interaction with SIR3. Proc. Natl. Acad. Sci. U.S.A. 94 2186-91 PubMed GONUTS page

[PMID:9150138-15] Kennedy, BK et al. (1997) Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell 89 381-91 PubMed GONUTS page

[PMID:9710643-16] Bourns, BD et al. (1998) Sir proteins, Rif proteins, and Cdc13p bind Saccharomyces telomeres in vivo. Mol. Cell. Biol. 18 5600-8 PubMed GONUTS page

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YEAST:SIR4

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