PMID:9150138

Citation	Kennedy, BK, Gotta, M, Sinclair, DA, Mills, K, McNabb, DS, Murthy, M, Pak, SM, Laroche, T, Gasser, SM and Guarente, L (1997) Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell 89:381-91
Abstract	A prior genetic study indicated that activity of Sir silencing proteins at a hypothetical AGE locus is essential for long life span. In this model, the SIR4-42 mutation would direct the Sir protein complex to the AGE locus, giving rise to a long life span. We show by indirect immunofluorescence that Sir3p and Sir4p are redirected to the nucleolus in the SIR4-42 mutant. Furthermore, this relocalization is dependent on both UTH4 a novel yeast gene that extends life span, and its homologue YGL023. Strikingly, the Sir complex is relocalized from telomeres to the nucleolus in old wild-type cells. We propose that the rDNA is the AGE locus and that nucleolar function is compromised in old yeast cells in a way that may be mitigated by targeting of Sir proteins to the nucleolus.
Links	PubMed
Keywords	Cell Aging/physiology; Cell Cycle Proteins; Cell Nucleolus/chemistry; Cell Nucleolus/metabolism; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Regulation, Fungal/physiology; Genes, Fungal/physiology; Molecular Sequence Data; Mutagenesis/physiology; RNA-Binding Proteins; Repressor Proteins; Saccharomyces cerevisiae/chemistry; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae/ultrastructure; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Silent Information Regulator Proteins, Saccharomyces cerevisiae; Telomere/chemistry; Telomere/metabolism; Trans-Activators/metabolism
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