YEAST:PIF1

Species (Taxon ID)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292)
Gene Name(s)	PIF1 (ECO:0000255 with HAMAP-Rule:MF_03176) (synonyms: TST1)
Protein Name(s)	ATP-dependent DNA helicase PIF1 (ECO:0000255 with HAMAP-Rule:MF_03176) DNA repair and recombination helicase PIF1 (ECO:0000255 with HAMAP-Rule:MF_03176) Petite integration frequency protein 1 Telomere stability protein 1
External Links
UniProt	P07271
EMBL	X05342 Z46729 Z38114 BK006946
PIR	A29457
RefSeq	NP_013650.1
ProteinModelPortal	P07271
BioGrid	35105
DIP	DIP-4448N
IntAct	P07271
MINT	MINT-496209
iPTMnet	P07271
PRIDE	P07271
EnsemblFungi	YML061C
GeneID	854941
KEGG	sce:YML061C
EuPathDB	FungiDB:YML061C
SGD	S000004526
GeneTree	ENSGT00530000063561
InParanoid	P07271
KO	K15255
OMA	WENIGAL
OrthoDB	EOG092C600K
BioCyc	YEAST:G3O-32656-MONOMER
BRENDA	3.6.4.12
PRO	PR:P07271
Proteomes	UP000002311
GO	GO:0005743 GO:0031966 GO:0005739 GO:0043596 GO:0005730 GO:0005634 GO:0005657 GO:0005524 GO:0043141 GO:0003678 GO:0051880 GO:0003697 GO:0010521 GO:0051276 GO:0006310 GO:0000733 GO:0000727 GO:0044806 GO:0000002 GO:0032211 GO:0071932 GO:0000723 GO:0000722
HAMAP	MF_03176
InterPro	IPR003840 IPR010285 IPR027417
PANTHER	PTHR23274
Pfam	PF05970
SUPFAM	SSF52540

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
part_of	GO:0005739	mitochondrion	PMID:16823961^[1]	ECO:0007005	high throughput direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0005739	mitochondrion	PMID:14576278^[2]	ECO:0007005	high throughput direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0005657	replication fork	PMID:26411679^[3]	ECO:0000314	direct assay evidence used in manual assertion		C	exists_during:(GO:0043111)	Seeded From UniProt	complete
involved_in	GO:0071932	replication fork reversal	PMID:26411679^[3]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000001073	P		Seeded From UniProt	complete
part_of	GO:0043596	nuclear replication fork	PMID:26981397^[4]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
involved_in	GO:0000733	DNA strand renaturation	PMID:25393406^[5]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0003697	single-stranded DNA binding	PMID:23596008^[6]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0051880	G-quadruplex DNA binding	PMID:23657261^[7]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0051276	chromosome organization	PMID:8287473^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0044806	G-quadruplex DNA unwinding	PMID:23657261^[7]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0032211	negative regulation of telomere maintenance via telomerase	PMID:16121131^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0032211	negative regulation of telomere maintenance via telomerase	PMID:16121131^[9]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0031966	mitochondrial membrane	PMID:20655619^[10]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
enables	GO:0010521	telomerase inhibitor activity	PMID:16121131^[9]	ECO:0000315	mutant phenotype evidence used in manual assertion		F		Seeded From UniProt	complete
enables	GO:0010521	telomerase inhibitor activity	PMID:16121131^[9]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	PMID:1849081^[11]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0005739	mitochondrion	PMID:1849081^[11]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	PMID:8253734^[12]	ECO:0000305	curator inference used in manual assertion	GO:0003678	C		Seeded From UniProt	complete
enables	GO:0003678	DNA helicase activity	PMID:8253734^[12]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0000727	double-strand break repair via break-induced replication	PMID:24025772^[13]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:10926538^[14]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000722	telomere maintenance via recombination	PMID:23390378^[15]	ECO:0000316	genetic interaction evidence used in manual assertion	UniProtKB:P25454	P		Seeded From UniProt	complete
involved_in	GO:0000722	telomere maintenance via recombination	PMID:23390378^[15]	ECO:0000316	genetic interaction evidence used in manual assertion	UniProtKB:P12753	P		Seeded From UniProt	complete
involved_in	GO:0000722	telomere maintenance via recombination	PMID:23390378^[15]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000006657	P		Seeded From UniProt	complete
involved_in	GO:0000002	mitochondrial genome maintenance	PMID:15907372^[16]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0043141	ATP-dependent 5'-3' DNA helicase activity	PMID:21873635^[17]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN001149889 PomBase:SPBC887.14c UniProtKB:Q9H611	F		Seeded From UniProt	complete
involved_in	GO:0032508	DNA duplex unwinding	PMID:21873635^[17]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN001149889 PomBase:SPBC887.14c	P		Seeded From UniProt	complete
involved_in	GO:0006260	DNA replication	PMID:21873635^[17]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN001149889 SGD:S000001073	P		Seeded From UniProt	complete
part_of	GO:0005657	replication fork	PMID:21873635^[17]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN001149889 SGD:S000001073 SGD:S000004526	C		Seeded From UniProt	complete
involved_in	GO:0051974	negative regulation of telomerase activity	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0010521	P		Seeded From UniProt	complete
involved_in	GO:0051974	negative regulation of telomerase activity	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0010521	P		Seeded From UniProt	complete
involved_in	GO:0051974	negative regulation of telomerase activity	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0010521	P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR010285	P		Seeded From UniProt	complete
enables	GO:0003678	DNA helicase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR010285	F		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR010285	P		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	C		Seeded From UniProt	complete
enables	GO:0043141	ATP-dependent 5'-3' DNA helicase activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	F		Seeded From UniProt	complete
involved_in	GO:0000002	mitochondrial genome maintenance	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	P		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	F		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	P		Seeded From UniProt	complete
involved_in	GO:0032211	negative regulation of telomere maintenance via telomerase	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	P		Seeded From UniProt	complete
enables	GO:0051880	G-quadruplex DNA binding	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	F		Seeded From UniProt	complete
part_of	GO:0005739	mitochondrion	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	C		Seeded From UniProt	complete
enables	GO:0010521	telomerase inhibitor activity	GO_REF:0000104	ECO:0000256	match to sequence model evidence used in automatic assertion	UniRule:UR000112749	F		Seeded From UniProt	complete
enables	GO:0004386	helicase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0347	F		Seeded From UniProt	complete
part_of	GO:0016020	membrane	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0539	C		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0233	P		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0234	P		Seeded From UniProt	complete
involved_in	GO:0006974	cellular response to DNA damage stimulus	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0227	P		Seeded From UniProt	complete
part_of	GO:0005743	mitochondrial inner membrane	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0999 UniProtKB-SubCell:SL-0168	C		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
part_of	GO:0005739	mitochondrion	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0496	C		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
part_of	GO:0005730	nucleolus	GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-SubCell:SL-0188	C		Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ Reinders, J et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J. Proteome Res. 5 1543-54 PubMed GONUTS page
↑ Sickmann, A et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. U.S.A. 100 13207-12 PubMed GONUTS page
↑ ^3.0 ^3.1 Rossi, SE et al. (2015) Rad53-Mediated Regulation of Rrm3 and Pif1 DNA Helicases Contributes to Prevention of Aberrant Fork Transitions under Replication Stress. Cell Rep 13 80-92 PubMed GONUTS page
↑ Rossi, SE et al. (2016) Genome-wide localization of Rrm3 and Pif1 DNA helicases at stalled active and inactive DNA replication forks of Saccharomyces cerevisiae. Genom Data 7 162-5 PubMed GONUTS page
↑ Ramanagoudr-Bhojappa, R et al. (2014) Yeast Pif1 accelerates annealing of complementary DNA strands. Biochemistry 53 7659-69 PubMed GONUTS page
↑ Ramanagoudr-Bhojappa, R et al. (2013) Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA. J. Biol. Chem. 288 16185-95 PubMed GONUTS page
↑ ^7.0 ^7.1 Paeschke, K et al. (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 497 458-62 PubMed GONUTS page
↑ Schulz, VP & Zakian, VA (1994) The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation. Cell 76 145-55 PubMed GONUTS page
↑ ^9.0 ^9.1 ^9.2 ^9.3 Boulé, JB et al. (2005) The yeast Pif1p helicase removes telomerase from telomeric DNA. Nature 438 57-61 PubMed GONUTS page
↑ Cheng, X & Ivessa, AS (2010) Association of the yeast DNA helicase Pif1p with mitochondrial membranes and mitochondrial DNA. Eur. J. Cell Biol. 89 742-7 PubMed GONUTS page
↑ ^11.0 ^11.1 Lahaye, A et al. (1991) PIF1: a DNA helicase in yeast mitochondria. EMBO J. 10 997-1007 PubMed GONUTS page
↑ ^12.0 ^12.1 Lahaye, A et al. (1993) PIF1 DNA helicase from Saccharomyces cerevisiae. Biochemical characterization of the enzyme. J. Biol. Chem. 268 26155-61 PubMed GONUTS page
↑ Saini, N et al. (2013) Migrating bubble during break-induced replication drives conservative DNA synthesis. Nature 502 389-92 PubMed GONUTS page
↑ Zhou, J et al. (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289 771-4 PubMed GONUTS page
↑ ^15.0 ^15.1 ^15.2 Hu, Y et al. (2013) Telomerase-null survivor screening identifies novel telomere recombination regulators. PLoS Genet. 9 e1003208 PubMed GONUTS page
↑ O'Rourke, TW et al. (2005) Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability. Gene 354 86-92 PubMed GONUTS page
↑ ^17.0 ^17.1 ^17.2 ^17.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:16823961-1] Reinders, J et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J. Proteome Res. 5 1543-54 PubMed GONUTS page

[PMID:14576278-2] Sickmann, A et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc. Natl. Acad. Sci. U.S.A. 100 13207-12 PubMed GONUTS page

[PMID:26411679-3] 3.0 ^3.1 Rossi, SE et al. (2015) Rad53-Mediated Regulation of Rrm3 and Pif1 DNA Helicases Contributes to Prevention of Aberrant Fork Transitions under Replication Stress. Cell Rep 13 80-92 PubMed GONUTS page

[PMID:26981397-4] Rossi, SE et al. (2016) Genome-wide localization of Rrm3 and Pif1 DNA helicases at stalled active and inactive DNA replication forks of Saccharomyces cerevisiae. Genom Data 7 162-5 PubMed GONUTS page

[PMID:25393406-5] Ramanagoudr-Bhojappa, R et al. (2014) Yeast Pif1 accelerates annealing of complementary DNA strands. Biochemistry 53 7659-69 PubMed GONUTS page

[PMID:23596008-6] Ramanagoudr-Bhojappa, R et al. (2013) Yeast Pif1 helicase exhibits a one-base-pair stepping mechanism for unwinding duplex DNA. J. Biol. Chem. 288 16185-95 PubMed GONUTS page

[PMID:23657261-7] 7.0 ^7.1 Paeschke, K et al. (2013) Pif1 family helicases suppress genome instability at G-quadruplex motifs. Nature 497 458-62 PubMed GONUTS page

[PMID:8287473-8] Schulz, VP & Zakian, VA (1994) The saccharomyces PIF1 DNA helicase inhibits telomere elongation and de novo telomere formation. Cell 76 145-55 PubMed GONUTS page

[PMID:16121131-9] 9.0 ^9.1 ^9.2 ^9.3 Boulé, JB et al. (2005) The yeast Pif1p helicase removes telomerase from telomeric DNA. Nature 438 57-61 PubMed GONUTS page

[PMID:20655619-10] Cheng, X & Ivessa, AS (2010) Association of the yeast DNA helicase Pif1p with mitochondrial membranes and mitochondrial DNA. Eur. J. Cell Biol. 89 742-7 PubMed GONUTS page

[PMID:1849081-11] 11.0 ^11.1 Lahaye, A et al. (1991) PIF1: a DNA helicase in yeast mitochondria. EMBO J. 10 997-1007 PubMed GONUTS page

[PMID:8253734-12] 12.0 ^12.1 Lahaye, A et al. (1993) PIF1 DNA helicase from Saccharomyces cerevisiae. Biochemical characterization of the enzyme. J. Biol. Chem. 268 26155-61 PubMed GONUTS page

[PMID:24025772-13] Saini, N et al. (2013) Migrating bubble during break-induced replication drives conservative DNA synthesis. Nature 502 389-92 PubMed GONUTS page

[PMID:10926538-14] Zhou, J et al. (2000) Pif1p helicase, a catalytic inhibitor of telomerase in yeast. Science 289 771-4 PubMed GONUTS page

[PMID:23390378-15] 15.0 ^15.1 ^15.2 Hu, Y et al. (2013) Telomerase-null survivor screening identifies novel telomere recombination regulators. PLoS Genet. 9 e1003208 PubMed GONUTS page

[PMID:15907372-16] O'Rourke, TW et al. (2005) Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability. Gene 354 86-92 PubMed GONUTS page

[PMID:21873635-17] 17.0 ^17.1 ^17.2 ^17.3 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

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YEAST:PIF1

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