YEAST:KU70

Species (Taxon ID)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292)
Gene Name(s)	YKU70 (synonyms: HDF1, NES24)
Protein Name(s)	ATP-dependent DNA helicase II subunit 1 ATP-dependent DNA helicase II subunit Ku70 High affinity DNA-binding factor subunit 1
External Links
UniProt	P32807
EMBL	X70379 D15052 AM296304 AM296302 AM296301 AM296299 AM296298 AM296297 AM296296 AM296294 AM296292 AM296295 AM296303 AM296293 Z49704 AY692863 BK006946
PIR	S54591
RefSeq	NP_014011.1
ProteinModelPortal	P32807
SMR	P32807
BioGrid	35464
DIP	DIP-2483N
IntAct	P32807
MINT	MINT-619653
iPTMnet	P32807
MaxQB	P32807
PeptideAtlas	P32807
PRIDE	P32807
EnsemblFungi	[example_ID YMR284W]
GeneID	855328
KEGG	sce:YMR284W
EuPathDB	FungiDB:YMR284W
SGD	S000004897
GeneTree	ENSGT00390000001422
InParanoid	P32807
KO	K10884
OMA	KPPYLKL
OrthoDB	EOG747PSQ
BioCyc	YEAST:G3O-32954-MONOMER
Reactome	R-SCE-5693571
NextBio	979046
PRO	PR:P32807
Proteomes	UP000002311
GO	GO:0043564 GO:0000790 GO:0005635 GO:0005724 GO:0005524 GO:0004003 GO:0003684 GO:0003723 GO:0042162 GO:0071480 GO:0071481 GO:0006333 GO:0006342 GO:0030466 GO:0032508 GO:0000727 GO:0000724 GO:0006303 GO:0097552 GO:0000723
Gene3D	1.10.1600.10 2.40.290.10 3.40.50.410
InterPro	IPR006165 IPR006164 IPR005160 IPR005161 IPR016194 IPR002035
PANTHER	PTHR12604:SF2
Pfam	PF02735 PF03730 PF03731
PIRSF	PIRSF003033
SMART	SM00559
SUPFAM	SSF100939 SSF53300

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
involved_in	GO:0097695	establishment of protein-containing complex localization to telomere	PMID:15531893^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	has_input:(GO:0005697) happens_during:(GO:0000080)	Seeded From UniProt	complete
involved_in	GO:0099114	chromatin silencing at subtelomere	PMID:26587833^[2]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:9501103^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:8604297^[4]	ECO:0000316	genetic interaction evidence used in manual assertion	UniProtKB:P38110	P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:8604297^[4]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:10818099^[5]	ECO:0000316	genetic interaction evidence used in manual assertion	UniProtKB:Q04437	P		Seeded From UniProt	complete
involved_in	GO:0006303	double-strand break repair via nonhomologous end joining	PMID:9501103^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0097552	mitochondrial double-strand break repair via homologous recombination	PMID:22214610^[6]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0043564	Ku70:Ku80 complex	PMID:8754818^[7]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
involved_in	GO:0030466	chromatin silencing at silent mating-type cassette	PMID:18791224^[8]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000001809	P		Seeded From UniProt	complete
involved_in	GO:0030466	chromatin silencing at silent mating-type cassette	PMID:18791224^[8]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006348	chromatin silencing at telomere	PMID:9501103^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006333	chromatin assembly or disassembly	PMID:11553718^[9]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0006303	double-strand break repair via nonhomologous end joining	PMID:10908335^[10]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
part_of	GO:0005635	nuclear envelope	PMID:10638763^[11]	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
enables	GO:0070034	telomerase RNA binding	PMID:12975323^[12]	ECO:0000314	direct assay evidence used in manual assertion		F	has_direct_input:(SGD:S000006657)	Seeded From UniProt	complete
involved_in	GO:0000727	double-strand break repair via break-induced replication	PMID:17321803^[13]	ECO:0000316	genetic interaction evidence used in manual assertion	SGD:S000000897 SGD:S000004837	P		Seeded From UniProt	complete
involved_in	GO:0000727	double-strand break repair via break-induced replication	PMID:17321803^[13]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000724	double-strand break repair via homologous recombination	PMID:11016833^[14]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:10818099^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0071481	cellular response to X-ray	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000288287 RGD:2643 RGD:3976	P		Seeded From UniProt	complete
involved_in	GO:0071480	cellular response to gamma radiation	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0041627 PANTHER:PTN000288287 UniProtKB:P12956 UniProtKB:P13010	P		Seeded From UniProt	complete
part_of	GO:0043564	Ku70:Ku80 complex	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0011774 PANTHER:PTN000288287 SGD:S000004712 SGD:S000004897 UniProtKB:P12956 UniProtKB:P13010 dictyBase:DDB_G0286069 dictyBase:DDB_G0286303	C		Seeded From UniProt	complete
enables	GO:0042162	telomeric DNA binding	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000288287 PomBase:SPBC543.03c PomBase:SPCC126.02c SGD:S000004712 UniProtKB:P13010	F		Seeded From UniProt	complete
involved_in	GO:0006303	double-strand break repair via nonhomologous end joining	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0011774 FB:FBgn0041627 MGI:MGI:95606 PANTHER:PTN000288287 PomBase:SPBC543.03c PomBase:SPCC126.02c SGD:S000004712 SGD:S000004897 TAIR:locus:2023757 TAIR:locus:2035495 UniProtKB:P12956 UniProtKB:P13010 UniProtKB:Q4WI96 dictyBase:DDB_G0286303	P		Seeded From UniProt	complete
contributes_to	GO:0003690	double-stranded DNA binding	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	MGI:MGI:95606 PANTHER:PTN000288287 RGD:2643 RGD:3976 TAIR:locus:2023757 TAIR:locus:2035495	F		Seeded From UniProt	complete
part_of	GO:0000784	nuclear chromosome, telomeric region	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	PANTHER:PTN000288287 PomBase:SPBC543.03c PomBase:SPCC126.02c SGD:S000004712 UniProtKB:P13010	C		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	PMID:21873635^[15]	ECO:0000318	biological aspect of ancestor evidence used in manual assertion	FB:FBgn0011774 FB:FBgn0041627 PANTHER:PTN000288287 PomBase:SPBC543.03c PomBase:SPCC126.02c SGD:S000004712 SGD:S000004897 TAIR:locus:2035495 UniProtKB:A0A1D8PF81 UniProtKB:Q7F1M0	P		Seeded From UniProt	complete
involved_in	GO:0032508	DNA duplex unwinding	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0004003	P		Seeded From UniProt	complete
part_of	GO:0099115	chromosome, subtelomeric region	GO_REF:0000108	ECO:0000364	evidence based on logical inference from manual annotation used in automatic assertion	GO:0099114	C		Seeded From UniProt	complete
involved_in	GO:0000723	telomere maintenance	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006165	P		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005160 InterPro:IPR006164 InterPro:IPR006165	F		Seeded From UniProt	complete
enables	GO:0003684	damaged DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006165	F		Seeded From UniProt	complete
enables	GO:0004003	ATP-dependent DNA helicase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005160	F		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006165	C		Seeded From UniProt	complete
involved_in	GO:0006303	double-strand break repair via nonhomologous end joining	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005160 InterPro:IPR006164 InterPro:IPR006165	P		Seeded From UniProt	complete
enables	GO:0042162	telomeric DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006165	F		Seeded From UniProt	complete
part_of	GO:0043564	Ku70:Ku80 complex	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR006165	C		Seeded From UniProt	complete
part_of	GO:0005724	nuclear telomeric heterochromatin	PMID:12080091^[16]	ECO:0000304	author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete
enables	GO:0003684	damaged DNA binding	PMID:10367891^[17]	ECO:0000304	author statement supported by traceable reference used in manual assertion		F		Seeded From UniProt	complete
part_of	GO:0000790	nuclear chromatin	PMID:10367891^[17]	ECO:0000304	author statement supported by traceable reference used in manual assertion		C		Seeded From UniProt	complete
involved_in	GO:0006974	cellular response to DNA damage stimulus	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0227	P		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
involved_in	GO:0006310	DNA recombination	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0233	P		Seeded From UniProt	complete
part_of	GO:0005694	chromosome	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0158	C		Seeded From UniProt	complete
involved_in	GO:0006281	DNA repair	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0234	P		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
enables	GO:0004386	helicase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0347	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0539 UniProtKB-SubCell:SL-0191	C		Seeded From UniProt	complete
part_of	GO:0000781	chromosome, telomeric region	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0779 UniProtKB-SubCell:SL-0276	C		Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ Fisher, TS et al. (2004) Cell cycle-dependent regulation of yeast telomerase by Ku. Nat. Struct. Mol. Biol. 11 1198-205 PubMed GONUTS page
↑ Larin, ML et al. (2015) Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin. PLoS Genet. 11 e1005425 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 Boulton, SJ & Jackson, SP (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17 1819-28 PubMed GONUTS page
↑ ^4.0 ^4.1 Porter, SE et al. (1996) The DNA-binding protein Hdf1p (a putative Ku homologue) is required for maintaining normal telomere length in Saccharomyces cerevisiae. Nucleic Acids Res. 24 582-5 PubMed GONUTS page
↑ ^5.0 ^5.1 Driller, L et al. (2000) A short C-terminal domain of Yku70p is essential for telomere maintenance. J. Biol. Chem. 275 24921-7 PubMed GONUTS page
↑ Kalifa, L et al. (2012) Mitochondrial genome maintenance: roles for nuclear nonhomologous end-joining proteins in Saccharomyces cerevisiae. Genetics 190 951-64 PubMed GONUTS page
↑ Milne, GT et al. (1996) Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol. Cell. Biol. 16 4189-98 PubMed GONUTS page
↑ ^8.0 ^8.1 Vandre, CL et al. (2008) The DNA end-binding protein Ku regulates silencing at the internal HML and HMR loci in Saccharomyces cerevisiae. Genetics 180 1407-18 PubMed GONUTS page
↑ Thrower, DA & Bloom, K (2001) Dicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeast. Mol. Biol. Cell 12 2800-12 PubMed GONUTS page
↑ Hegde, V & Klein, H (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res. 28 2779-83 PubMed GONUTS page
↑ Galy, V et al. (2000) Nuclear pore complexes in the organization of silent telomeric chromatin. Nature 403 108-12 PubMed GONUTS page
↑ Stellwagen, AE et al. (2003) Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends. Genes Dev. 17 2384-95 PubMed GONUTS page
↑ ^13.0 ^13.1 Krishna, S et al. (2007) Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replication. DNA Repair (Amst.) 6 797-808 PubMed GONUTS page
↑ Cervelli, T & Galli, A (2000) Effects of HDF1 (Ku70) and HDF2 (Ku80) on spontaneous and DNA damage-induced intrachromosomal recombination in Saccharomyces cerevisiae. Mol. Gen. Genet. 264 56-63 PubMed GONUTS page
↑ ^15.0 ^15.1 ^15.2 ^15.3 ^15.4 ^15.5 ^15.6 ^15.7 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
↑ Luo, K et al. (2002) Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast. Genes Dev. 16 1528-39 PubMed GONUTS page
↑ ^17.0 ^17.1 Martin, SG et al. (1999) Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast. Cell 97 621-33 PubMed GONUTS page

[PMID:15531893-1] Fisher, TS et al. (2004) Cell cycle-dependent regulation of yeast telomerase by Ku. Nat. Struct. Mol. Biol. 11 1198-205 PubMed GONUTS page

[PMID:26587833-2] Larin, ML et al. (2015) Competition between Heterochromatic Loci Allows the Abundance of the Silencing Protein, Sir4, to Regulate de novo Assembly of Heterochromatin. PLoS Genet. 11 e1005425 PubMed GONUTS page

[PMID:9501103-3] 3.0 ^3.1 ^3.2 Boulton, SJ & Jackson, SP (1998) Components of the Ku-dependent non-homologous end-joining pathway are involved in telomeric length maintenance and telomeric silencing. EMBO J. 17 1819-28 PubMed GONUTS page

[PMID:8604297-4] 4.0 ^4.1 Porter, SE et al. (1996) The DNA-binding protein Hdf1p (a putative Ku homologue) is required for maintaining normal telomere length in Saccharomyces cerevisiae. Nucleic Acids Res. 24 582-5 PubMed GONUTS page

[PMID:10818099-5] 5.0 ^5.1 Driller, L et al. (2000) A short C-terminal domain of Yku70p is essential for telomere maintenance. J. Biol. Chem. 275 24921-7 PubMed GONUTS page

[PMID:22214610-6] Kalifa, L et al. (2012) Mitochondrial genome maintenance: roles for nuclear nonhomologous end-joining proteins in Saccharomyces cerevisiae. Genetics 190 951-64 PubMed GONUTS page

[PMID:8754818-7] Milne, GT et al. (1996) Mutations in two Ku homologs define a DNA end-joining repair pathway in Saccharomyces cerevisiae. Mol. Cell. Biol. 16 4189-98 PubMed GONUTS page

[PMID:18791224-8] 8.0 ^8.1 Vandre, CL et al. (2008) The DNA end-binding protein Ku regulates silencing at the internal HML and HMR loci in Saccharomyces cerevisiae. Genetics 180 1407-18 PubMed GONUTS page

[PMID:11553718-9] Thrower, DA & Bloom, K (2001) Dicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeast. Mol. Biol. Cell 12 2800-12 PubMed GONUTS page

[PMID:10908335-10] Hegde, V & Klein, H (2000) Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast. Nucleic Acids Res. 28 2779-83 PubMed GONUTS page

[PMID:10638763-11] Galy, V et al. (2000) Nuclear pore complexes in the organization of silent telomeric chromatin. Nature 403 108-12 PubMed GONUTS page

[PMID:12975323-12] Stellwagen, AE et al. (2003) Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends. Genes Dev. 17 2384-95 PubMed GONUTS page

[PMID:17321803-13] 13.0 ^13.1 Krishna, S et al. (2007) Mre11 and Ku regulation of double-strand break repair by gene conversion and break-induced replication. DNA Repair (Amst.) 6 797-808 PubMed GONUTS page

[PMID:11016833-14] Cervelli, T & Galli, A (2000) Effects of HDF1 (Ku70) and HDF2 (Ku80) on spontaneous and DNA damage-induced intrachromosomal recombination in Saccharomyces cerevisiae. Mol. Gen. Genet. 264 56-63 PubMed GONUTS page

[PMID:21873635-15] 15.0 ^15.1 ^15.2 ^15.3 ^15.4 ^15.5 ^15.6 ^15.7 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page

[PMID:12080091-16] Luo, K et al. (2002) Rap1-Sir4 binding independent of other Sir, yKu, or histone interactions initiates the assembly of telomeric heterochromatin in yeast. Genes Dev. 16 1528-39 PubMed GONUTS page

[PMID:10367891-17] 17.0 ^17.1 Martin, SG et al. (1999) Relocalization of telomeric Ku and SIR proteins in response to DNA strand breaks in yeast. Cell 97 621-33 PubMed GONUTS page

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YEAST:KU70

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